天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (11): 1330-1333.doi: 10.11958/20160610

• 实验研究 • 上一篇    下一篇

下调 HER2 降低自噬活性抑制人肺癌细胞增殖 转移作用的体外研究

冯媛媛 1， 伍宏山 2， 张志宏 2， 龙新华 2， 周扬 2， 童未来 2， 刘志礼 2， 刘家明 2△   

  1. 1 南昌大学基础医学部 （邮编 330006）； 2 南昌大学第一附属医院骨科
  • 收稿日期:2016-07-05 修回日期:2016-08-30 出版日期:2016-11-15 发布日期:2016-11-15
  • 通讯作者: △通讯作者 E-mail: liujiamingdr@hotmail.com E-mail:liujiamingdr@hotmail.com
  • 作者简介:冯媛媛 （1994）， 女， 硕士研究生， 主要从事肿瘤的分子机制方面研究
  • 基金资助:
    江西省自然科学基金项目 （2014ZBAB205012， 20151BAB215026）

The inhibition of autophagy suppresses proliferation and metastasis of lung cancer cells via down-regulation of HER2

FENG Yuanyuan1, WU Hongshan2, ZHANG Zhihong2, LONG Xinhua2, ZHOU Yang2, TONG Weilai2, LIU Zhili2, LIU Jiaming2△   

  1. 1 Department of Basic Medicine, Nanchang University, Nanchang 330006, China; 2 Department of Orthopdeics, the First Affiliated Hospital of Nanchang University
  • Received:2016-07-05 Revised:2016-08-30 Published:2016-11-15 Online:2016-11-15
  • Contact: △Corresponding Author E-mail: liujiamingdr@hotmail.com E-mail:liujiamingdr@hotmail.com

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摘要： 目的 探讨下调 HER2 表达是否抑制细胞自噬活性， 从而影响人肺癌细胞的增殖、 迁徙和侵袭。方法 采用浓度 5 μmol/L 自噬抑制剂 3-MA、 10 μmol/L HER2 抑制剂 Neratinib 分别作用人肺癌细胞 A549。Western blot 检测肺癌细胞 A549 中 HER2、 Beclin-1 及 LC3B （Ⅱ/Ⅰ） 蛋白表达水平； Wound healing 和 Transwell invasion 实验检测 细胞迁徙、 侵袭能力； 四甲基偶氮唑盐 （MTT） 检测细胞的增殖能力。结果 Neratinib 和自噬抑制剂 （3-MA） 作用 24 h 后， 细胞 HER2、 Beclin-1 及 LC3B（Ⅱ/Ⅰ）蛋白表达水平显著低于阴性对照组； Neratinib、 自噬抑制剂（3-MA）处理组 的细胞增殖、 迁徙和侵袭能力显著低于阴性对照细胞。结论 下调 HER2 能降低人肺癌细胞 A549 的自噬活性并抑 制其增殖、 迁徙和侵袭

关键词: 肺肿瘤, 自噬, 细胞增殖, 受体, erbB-2, 细胞侵袭, 细胞迁徙

Abstract: Objective To investigate whether the down-regulation of HER2 can inhibit the autophagy of cells and influence the proliferation and metastasis of lung cancer cells. Methods The 5 μmol/L of autophagy inhibitor (3-MA) and 10 μmol/L of HER2 inhibitor (Neratinib) were used to treat human lung cancer A549 cells. Western blot assay was used to detect the protein expressions of HER2, Beclin-1 and LC3B (Ⅱ/Ⅰ) in A549 cells. Wound healing and Transwell invasion methods were used to detect the invasion and migration ability of A549 cells. MTT assay was performed to evaluate the cell proliferation. Results Western blot analysis showed that the protein expressions of HER2, Beclin-1 and LC3B (Ⅱ/Ⅰ) were significantly declined in A549 cells treated with Neratinib and 3-MA for 24 h compared to cells in control group. The cell proliferation, migration and invasion ability were significantly decreased in A549 cells treated with Neratinib and 3-MA thanthoseofcontrolcells.Conclusion TheinhibitionofHER2proteincansuppresstheautophagyandproliferationofA549cells.

Key words: lung neoplasms, autophagy, cell proliferation, receptor, erbB-2, cell invasion, cell migration