天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (12): 1500-1503.doi: 10.11958/20160854

• 临床研究 • 上一篇    下一篇

定量检测SLIT2 基因甲基化对高级别宫颈癌前病变的诊断价值

袁立芹1 ， 胡元晶2△   

  1. 1 天津医科大学 （邮编 300070）； 2 天津市中心妇产科医院
  • 收稿日期:2016-08-19 修回日期:2016-09-26 出版日期:2016-12-15 发布日期:2017-01-26
  • 通讯作者: 胡元晶 △通讯作者 E-mail: tdjhyj@hotmail.com E-mail:846956565@qq.com
  • 作者简介:袁立芹 （1987）， 女， 硕士在读， 主要从事妇科肿瘤方向研究
  • 基金资助:
    天津市卫生局科研课题资助项目 （13KG134）

Diagnostic value of quantitative detection of SLIT2 methylation for cervical high grade precancerous lesion

YUAN Liqin1 , HU Yuanjing2△   

  1. 1 Tianjin Medical University, Tianjin 300070, China; 2 Department of Gynecology and Obstetrics, Tianjin Central Hospital
  • Received:2016-08-19 Revised:2016-09-26 Published:2016-12-15 Online:2017-01-26
  • Contact: HU Yuanjing △Corresponding Author E-mail: tdjhyj@hotmail.com E-mail:846956565@qq.com

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摘要： 摘要:目的 探讨神经导向因子 2 （SLIT2） 基因甲基化定量检测对于高级别宫颈癌前病变的临床诊断价值。方法 收集 178 例高危型人乳头瘤病毒 （HPV） 感染患者的宫颈脱落细胞， 以组织病理学诊断作为金标准， 分为正常宫颈组 （n=45）、 低级别病变组 （n=50） 和高级别病变组 （n=83）。采用焦磷酸测序法定量检测各样本中 SLIT2 基因的甲基化水平。采用受试者工作特征 （ROC） 曲线确定 SLIT2 基因的甲基化水平对高级别宫颈癌前病变的诊断阈值。结果 正常宫颈组 SLIT2 基因的甲基化水平为 （4.53±1.37） %， 低级别病变组为 （5.81±2.26） %， 高级别病变组为 （11.80± 8.47） %， 组间差异有统计学意义 （F=27.61， P<0.001）； 高级别病变组高于正常宫颈组和低级别病变组 （均 P<0.001），正常宫颈组和低级别病变组之间差异无统计学意义 （P=0.297）。SLIT2 基因甲基化定量检测诊断高级别宫颈癌前病变的灵敏度为 80.7%， 特异度为 83.2%， 最佳界值为 6.41%。结论 采用焦磷酸测序法定量检测宫颈脱落细胞中 SLIT2 基因甲基化水平能够有效诊断高级别宫颈癌前病变。

关键词: 宫颈肿瘤, 癌前状态, DNA 甲基化, &alpha, 乳头状瘤病毒属, 宫颈癌前病变, 神经导向因子2

Abstract: Abstract： Objective To explore the clinical diagnostic value of quantitative detection of the slit homologue 2 (SLIT2） methylation for cervical high grade precancerous lesions. Methods According to histopathologic diagnostic results, 178 patients infected with high-risk HPV were divided into normal cervix group (n=45), low-grade lesion group (n=50) and high- grade lesion group (n=83). The cervical exfoliated cells were collected in three groups. The methylation levels of SLIT2 were measured by pyrosequencing in three groups. The diagnostic threshold of SLIT2 in high grade precancerous lesions was estimated by receiver operating characteristic (ROC) curve. Results The percentages of SLIT2 methylation were (4.53± 1.37)%, (5.81±2.26)% and (11.80±8.47)% in normal cervix group, low-grade lesion group and high-grade lesion group, respectively. And the differences between three groups were statistically significant (F=27.61, P<0.001). The percentage of SLIT2 methylation was significantly higher in high-grade lesion group than that of normal cervix group and low-grade lesion group (P<0.001). There was no significant difference in the percentage of SLIT2 methylation between normal cervix group and low-grade lesion group (P=0.297). The area under the ROC curve was 0.895 and optimal cut-off value was 6.41%. The sensitivity and specificity were 80.7% and 83.2%, respectively for the detection by SLIT2 methylation. Conclusion The quantitative detection of SLIT2 gene methylation level in cervical exfoliated cells by pyrosequencing can effectively diagnose cervical high grade precancerous lesions.

Key words: uterine cervical neoplasms, precancerous conditions, DNA methylation, alphapapillomavirus, cevical precancerous lesions, slit homologue 2