天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (6): 580-583.doi: 10.11958/20170457

• 实验研究 • 上一篇    下一篇

3,3′-二吲哚甲烷对小鼠造血系统辐射损伤的保护作用

董佳丽，路璐，樊赛军△   

  1. 中国医学科学院北京协和医学院放射医学研究所，天津市放射医学与分子核医学重点实验室（邮编 300192）
  • 收稿日期:2017-04-12 修回日期:2017-04-27 出版日期:2017-06-15 发布日期:2017-07-05
  • 通讯作者: △通讯作者 E-mail: fansaijun@irm-cams.ac.cn E-mail:0lulu0@163.com
  • 作者简介:董佳丽（1992），女，硕士在读，主要从事辐射损伤防护药物的机制研究
  • 基金资助:
    国 家 自 然 科 学 基 金 面 上 项 目（81071906，81172127，81572969）；天 津 市 应 用 基 础 与 前 沿 技 术 研 究 计 划 青 年 项 目 （15JCQNJC46000）；“协和青年基金资助”和“中央高校基本科研业务费专项资金资助”（3332013044）；中国医学科学院、北京协和医学院“中央 级公益性科研院所基本科研业务费”（2016RC310016）

Protective effects of 3,3′-diindolylmethane on radiation damage of hematopoietic system in mice

DONG Jia-li, LU Lu, FAN Sai-jun△   

  1. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China
  • Received:2017-04-12 Revised:2017-04-27 Published:2017-06-15 Online:2017-07-05
  • Contact: △Corresponding Author E-mail: fansaijun@irm-cams.ac.cn E-mail:0lulu0@163.com

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摘要： 目的 探讨 3,3′-二吲哚甲烷（3,3′-diindolylmethane, DIM）对 2 Gy 辐射损伤小鼠造血系统的保护作用。 方法 30 只雄性 C57BL/6 小鼠随机区组法分为对照组、2 Gy 照射组和 2 Gy 照射+DIM 组，每组 10 只。对照组小鼠 接受假照射（0 Gy），其余 2 组小鼠接受 2 Gy 137Cs γ-射线一次性全身照射。2 Gy 照射+DIM 组小鼠照射前 30 min 腹 腔注射 DIM（75 mg/kg），其余 2 组小鼠腹腔注射对照溶液。照后 7 d 和 15 d 每组取 5 只小鼠，取外周血进行血象测 定，取骨髓细胞测定骨髓有核细胞（BMNCs）数目，活性氧检测探针（DCFH-DA）检测细胞内活性氧（ROS）水平、骨髓 粒-单核巨噬细胞集落（CFU-GM）形成数目检测克隆形成能力。结果 与对照组比较，2 Gy 照射组外周血白细胞、 血小板数量、BMNCs 数目和克隆形成能力显著下降，细胞内 ROS 水平显著提高（P<0.05）。与 2 Gy 照射组比较，2 Gy 照射+DIM 组外周血白细胞、血小板数量、BMNCs 数目和克隆形成能力显著提高，细胞内 ROS 水平显著下降（P< 0.05）。结论 DIM 对小鼠 2 Gy 照射后造血系统损伤有一定的保护作用，其机制可能与降低骨髓细胞内 ROS 水平 有关。

关键词: 辐射损伤, 造血系统, 活性氧, 骨髓细胞, 小鼠, 近交 C57BL, 3,3′-二吲哚甲烷

Abstract: Objective To investigate the protective effect of 3,3′-diindolylmethane (DIM) on radiation-induced injury in mouse hematopoietic system. Methods Thirty C57BL/6 mice were randomly divided into control group, 2 Gy irradiation group and 2 Gy irradiation + DIM group (n=10 for each group). Mice of control group received sham irradiation, and the other two groups accepted 2 Gy 137Cs γ-ray total body irradiation. Mice in 2 Gy irradiation +DIM group were intraperitoneally injected 75 mg/kg DIM 30 min before irradiation. Mice of other two groups were treated with reference solution. After 7 d and 15 d of 2 Gy irradiation, the peripheral blood samples were collected to count the number of bone marrow nuclear cells (BMNCs). The level of reactive oxygen species(ROS) was measured by DCFH- DA. The levels of colony forming unitsgranulocyte-macrophage (CFU-GM) were also detected. Results The numbers of white blood cell (WBC), platelet count (PLT), BMNCs and CFU-GM were significantly decreased and the ROS level of bone marrow cells increased significantly in the irradiated group than those of control group (P＜0.05). Compared to 2 Gy irradiation group, the numbers of WBC, PLT, BMNCs and CFU-GM were significantly increased in 2 Gy irradiation +DIM group, and the level of ROS was decreased significantly (P＜0.05). Conclusion DIM has a protective effect on hematopoietic cells following radiation-induced injury, which may be related with the decreased ROS level.

Key words: radiation injuries, hematopoietic system, reactive oxygen species, bone marrow cells, mice, inbred C57BL, 3,3′-diindolylmethane