天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (7): 704-708.doi: 10.11958/20170045

• 临床研究 • 上一篇    下一篇

HIF-1α 和 Hp 在蒙、 汉两族胃癌中的表达及意义

冷雪芹，李燕，贾慧琼 ，胡香珍 ，段晓燕 ，鲁志刚   

  1. 1 内蒙古医科大学附属医院消化内科（邮编 010051）； 2 乌海市人民医院消化内科； 3 内蒙古医科大学附属医院高压氧科， 4 胸外科
  • 收稿日期:2017-01-10 修回日期:2017-05-17 出版日期:2017-07-15 发布日期:2017-08-08
  • 通讯作者: 冷雪芹 E-mail:lxq63@aliyun.com
  • 基金资助:
    内蒙古自治区自然科学基金 （2014MS08109）

The expression and the clinical guiding significance of HIF-1α and Hp in Mongolian and Han two nationalities

LENG Xue-Qin,LI Yan,JIA Hui-Qiong,HU Xiang-Zhen,DUAN Xiao-Yan,LU Zhi-Gang   

  1. 1 Department of Gastroenterology, the Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia Huhhot 010051, China; 2 Department of Gastroenterology, People’ s Hospital of Wuhai; 3 Department of Hyperbaric Oxygen, 4 Department of Thoracic Surgery,the Affiliated Hospital of Inner Mongolia Medical University
  • Received:2017-01-10 Revised:2017-05-17 Published:2017-07-15 Online:2017-08-08

PDF (PC)

3815

摘要： 目的 分析缺氧诱导因子 （HIF） -1α 和幽门螺旋杆菌 （Hp） 在蒙古族、 汉族胃癌组织中的表达， 探讨两种因子在蒙、 汉两族胃癌发生中的相互作用。方法 采用改良的 Warthin-Starry（W-S）银染色法检测 27 例蒙古族和 30例汉族胃癌患者 Hp 感染情况， 采用免疫组化（S-P）法、 逆转录聚合酶链反应（RT-PCR）法分别检测 HIF-1α 在癌组织、 癌旁组织和正常组织中蛋白和 mRNA 的表达。分析 HIF-1α 蛋白和 Hp 在蒙古族、 汉族胃癌组织中表达的关系。结果 蒙古族、 汉族胃癌患者 Hp 感染率分别为 81.5% （22/27）、 66.7% （20/30）， 差异无统计学意义 （χ2=1.608， P＞0.05）； HIF-1α 蛋白在蒙古族、 汉族胃癌患者癌组织中的阳性表达率分别为 62.96%（17/27）、 70.00%（21/30）， mRNA相对表达量分别为 0.891 4±0.075 0、 0.907 4±0.065 5， 两民族间差异无统计学意义； HIF-1α 蛋白和 mRNA 在癌旁和正常组织中均无阳性表达。蒙古族、 汉族人群胃癌组织中 HIF-1α 蛋白的阳性表达与 Hp 感染均呈正相关 （rs分别为0.424、 0.617， P＜0.05）。结论 HIF-1α 和 Hp 均是胃癌的致病因子， Hp 和 HIF-1ɑ 可能共同促进胃癌的发生。HIF-1α 和 Hp 在蒙古族和汉族胃癌患者中的致病机制可能相同。

关键词: 胃肿瘤, 缺氧诱导因子 1, α 亚基, 螺杆菌, 幽门, 免疫组织化学, 逆转录聚合酶链反应, 汉族, 蒙古族

Abstract: Objective To detect the expressions of two protein factors hypoxia- inducible factor (HIF)- 1α and Helicobacter pylori (Hp) in gastric cancer in Mongolian and Han two nationalities respectively, and to explore the relationship of HIF-1α and Hp in the development of gastric cancer. Methods A modified Warthin-Starry (W-S) silver staining method was used to detect Hp infection in 27 Mongolian patients with gastric cancer and 30 Han patients with gastric cancer. Immunohistochemical (S-P) method and reverse transcription polymerase chain reaction (RT-PCR) method were used to detect protein and mRNA expressions of HIF- 1α in cancer tissue, paracancerous tissue and normal tissue samples. The relationship of HIF-1α protein and Hp expression was analyzed between gastric carcinoma tissue samples of Mongolian patient group and Han patient group. Results Hp infection rates were 81.5% (22/27) and 66.7% (20/30) in Mongolian and Han patients with gastric cancer. The difference in infection rate was not statistically significant between two groups of patients (χ2=1.608, P > 0.05). The positive expression levels of HIF-1α protein were 62.96% (17/27) and 70.00% (21/30) in gastric cancer tissue samples of Mongolian and Han groups. The relative expression levels of HIF-1α mRNA were 0.891 4±0.075 0 and 0.907 4±0.065 5. There was no significant difference in the HIF-1α mRNA expression between the two nationalities. No positive expressions of HIF-1α protein and mRNA were found in paracancerous tissue and normal tissue samples. There was a positive correlation in the positive expression of HIF- 1α protein and Hp infection in gastric cancer tissue samples of Mongolian and Han patient groups (rs =0.424 and 0.617, P < 0.05). Conclusion HIF-1α and Hp are pathogenic factors of gastric cancer. Hp and HIF-1α may promote the occurrence of gastric cancer together. The pathogenic mechanism of HIF-1α and Hp in gastric cancer may be the same for Mongolian and Han patients.

Key words: stomach neoplasms, hypoxia-inducible factor 1, alpha subunit, helicobacter pylori, immunohistochemistry, reverse transcriptase polymerase chain reaction, Han nationality, Mongolian nationality

中图分类号: