天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (11): 1155-1160.doi: 10.11958/20180712

• 实验研究 • 上一篇    下一篇

清胰汤和姜黄素调整肠道微生态对重症急性胰腺炎的治疗机制

胡炜1 ， 刘洪斌2， 3△， 王曼雪1 ， 张桂贤2 ， 李东华3 ， 张一3   

  1. 1天津医科大学研究生院 （邮编300070）； 2天津市医药科学研究所； 3天津市南开医院
  • 收稿日期:2018-05-04 修回日期:2018-08-30 出版日期:2018-11-15 发布日期:2018-11-20
  • 通讯作者: 胡炜 E-mail:1273980198@qq.com
  • 基金资助:
    清胰汤调控 MTD-FPR/TLR9-器官损伤信号通路防治重症急性胰 腺炎早期 MODS 作用机制

The mechanism of QingYiTang or Turmeric for adjusting the intestinal microecology after severe acute pancreatitis

HU Wei 1 , LIU Hong-bin2， 3△, WANG Man-xue1 , ZHANG Gui-xian2 , LI Dong-hua3 , ZHANG Yi 3   

  1. 1 Tianjin Medical University, Tianjin 300070, China; 2 Tianjin Institute of Medical & Pharmaceutical Sciences; 3 Tianjin Nankai Hospital
  • Received:2018-05-04 Revised:2018-08-30 Published:2018-11-15 Online:2018-11-20
  • Supported by:
    Effects of Qing-Yi-Tang on the pathway of MTD-FPR/TLR9 in preventing MODS of early severe acute pancreatitis

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摘要： 摘要： 目的 探讨清胰汤和姜黄素调整肠道微生态治疗重症急性胰腺炎 （SAP） 的机制。方法 40只SPF级健康 Wistar大鼠， 按照随机数字法分为4组， 对照组、 模型组、 清胰汤组、 姜黄素组， 每组10只。通过注射牛磺胆酸钠 （Na- Fc）， 诱发大鼠形成SAP模型， 建模前清胰汤组、 姜黄素组分别用相应中药汤剂灌胃1周。采用苏木素-伊红 （HE） 染色法观察结肠和胰腺组织病理变化， 收集大鼠盲肠段粪便， 16S rDNA测序技术分析4组大鼠肠道微生物菌群的变化。结果 病理结果显示， 模型组肠组织表现为肠壁水肿， 肠黏膜上皮缺损、 脱落等， 上皮下间质空隙变宽， 大量的中性粒细胞 （PMN） 等炎性细胞浸润等； 胰腺组织表现为胰腺腺泡水肿、 坏死， 叶间隔增宽， 大量的PMN等炎性细胞浸润等， 而中药清胰汤和姜黄素组可减轻模型组中的胰腺损伤和肠黏膜受损程度 （P＜0.05）。肠道菌群微生态结果显示清胰汤或 （和） 姜黄素组可有效降低SAP大鼠厚壁菌门 （包括梭菌纲、 芽孢杆菌纲）、 毛螺菌属数量菌群含量， 升高拟杆菌门 （包括拟杆菌纲、 拟杆菌属） 和乳酸杆菌属含量。结论 中药清胰汤和姜黄素可增加SAP大鼠的肠道菌群的多样性和丰富性， 调控微生物生态平衡， 增加益生菌群的含量， 降低有害菌群的定植能力， 从而达到对肠道的保护作用。

关键词: 胰腺炎, 急性坏死性, 清胰汤, 姜黄素, 肠道菌群, 16S rDNA测序

Abstract: Abstract: Objective To explore the treatment mechanism of severe acute pancreatitis (SAP) by adjusting the intestinal microecology of QingYiTang or Turmeric. Methods According to the random number method， forty healthy Wistar rats with SPF grade were divided into four groups, with 10 rats in each group, respectively named the control group, the model group, the QingYiTang group, and the Turmeric group. Na-Fc was injected to induce SAP model rats, before the modeling, QingYiTang and Turmeric group were respectively given corresponding traditional Chinese medicine decoctionin by tragastric administration for 1 week. The pathological changes of colon and pancreas tissues were observed by hematoxylin-eosin (HE) staining. Feces from caecal segments of rats were collected, and changes of intestinal microbial flora were analyzed by 16S rDNA sequencing technique in four groups. Results (1) According to the pathological results, the intestinal tissue of model group showed edema of intestinal wall, epithelial defect and exfoliation of intestinal mucosa, widening of interstitial space under epithelium, a large number of inflammatory cells, such as PMN infiltration. Pancreatic tissues showed pancreatic acinar edema, necrosis, enlarged septa, a large number of PMN and other inflammatory cell infiltration. The QingYiTang and Turmeric groups showed reduced pancreatic injury and intestinal mucosa damage (P＜ 0.05). (2)The results of the microecology of intestinal flora showed that the richness of firmicutes (including clostridium and bacillus), and spirulina were effectively reduced in SAP rats, otherwise the richness of bacteroides (including bacteroides and bacteroides) and lactobacillus were increased. Conclusion QingYiTang and Turmeric can increase intestinal flora diversity and richness in SAP rats, regulate the microbial ecological balance, increase the amount of probiotic group, reduce harmful bacteria colonize ability, so as to achieve the protection of intestinal function.

Key words: pancreatitis,acute necrotizing, qingyitang, turmeric, intestinal flora, 16SrDNA sequencing