关键词: 神经元, 铁, 转染, Ndfip1, 神经保护

Abstract: Abstract: Objective To investigate the protective mechanism of Ndfip1 overexpression on iron overload injury in nerve cells (SH-SY5Y cells). Methods The SH-SY5Y cells were used as the cell model, and the Ndfip1 plasmid was successfully constructed. SH-SY5Y cells were transfected with Ndfip1 plasmid or empty plasmid, respectively, and the experimental group and the control group were established. The MTT assay was used to detect changes of survival rates of SH-SY5Y cells under different concentrations of FeSO4, and the iron overload concentration was determined. SH-SY5Y cells were transfected with Ndfip1 plasmid to determine the transfection efficiency. Ndfip1 plasmid and empty plasmid were transfected into SH-SY5Y cells, respectively. The survival rates of the two groups were detected by MTT method. The fluorescence intensity was observed by fluorescence microscopy using Calcein AM method to determine the intracellular iron ion contents in two groups of cells. The fluorescence intensity was detected by fluorescence spectrophotometer to determine the changes of iron uptake in two groups of cells. Results As the concentration of FeSO4 increased, the survival rates of SH-SY5Y cells gradually decreased. The 200 μmol/L FeSO4 can cause a significant decrease in survival rate of SH-SY5Y cells. The transfection of SH-SY5Y cells with Ndfip1 plasmid significantly increased the expression of Ndfip1. The overexpression of Ndfip1 can increase the survival rate of iron overload injury, reduce intracellular iron content, and reduce the uptake of iron ions in SH-SY5Y cells. Conclusion Iron overload causes nerve cells to damage, resulting in the decrease in survival rate. Ndfip1 can reduce iron ions into nerve cells, reduce iron accumulation in nerve cells, reduce iron damage to nerve cells, improve survival rate, and play its protective role.

Key words: neurons, iron, transfection, Ndfip1, neuroprotection