天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (4): 417-420.doi: 10.11958/20181809

• 临床研究 • 上一篇    下一篇

NOD2、TNF-α、MMP-9及Caspase-3在胎膜早破 发病中的作用及意义

宋春红 1，马倩 2，甄娟 1，许红 1，刘洋 1，毕学杰 1△   

  1. 1石家庄市第四医院病理科（邮编 050011）；2深州市医院妇产科
  • 收稿日期:2018-11-20 修回日期:2019-02-22 出版日期:2019-04-15 发布日期:2019-05-27
  • 通讯作者: 宋春红 E-mail:trirainbow@126.com
  • 作者简介:宋春红（1979），女，硕士，副主任医师，主要从事妇产科病理研究
  • 基金资助:
    河北省“三三三人才工程”资助项目;河北省医学科学研究重点课题计划

The role and significance of NOD2, TNF-α, MMP-9 and Caspase-3 in the pathogenesis of premature rupture of the membranes

SONG Chun-hong1, MA Qian2, ZHEN Juan1, XU Hong1, LIU Yang1, BI Xue-jie1△   

  1. 1 Department of Pathology, the Fourth Hospital of Shijiazhuang, Shijiazhuang 050011, China; 2 Department of Gynaecology and Obstetrics, Shenzhou Hospital
  • Received:2018-11-20 Revised:2019-02-22 Published:2019-04-15 Online:2019-05-27

摘要： 目的 探讨胎膜早破患者胎膜组织核苷酸结合寡聚化结构域受体 2（NOD2）与基质金属蛋白酶-9（MMP- 9）、活化型 Caspase-3蛋白及羊水中肿瘤坏死因子-α（TNF-α）水平表达的相关性，为进一步了解胎膜早破的病因学 及治疗途径提供新思路。方法 选择足月胎膜早破、未足月胎膜早破、正常妊娠晚期妇女各 30例，应用免疫组织化 学染色法及蛋白印迹技术检测各组胎膜组织 NOD2、MMP-9及活化型 Caspase-3的蛋白表达部位及水平，ELISA法检 测羊水中 TNF-α水平，并分析四者表达的相关性。结果 胎膜早破组胎膜组织 NOD2、MMP-9、活化型 Caspase-3表 达及羊水 TNF-α水平高于正常妊娠晚期组（P＜0.05），未足月胎膜早破组 NOD2表达及羊水 TNF-α水平高于足月胎 膜早破组（P＜0.05），MMP-9及活化型 Caspase-3在足月胎膜早破组胎膜组织中的表达与未足月胎膜早破组相比差 异无统计学意义。胎膜组织中 NOD2表达水平与羊水 TNF-α水平、胎膜组织中 MMP-9及活化型 Caspase-3的表达呈 正相关（均 P＜0.01）。结论 NOD2、TNF-α、MMP-9、活化型 Caspase-3表达水平升高与胎膜早破的发生相关；四者 在胎膜早破发病机制中存在关联。

关键词: 胎膜早破, Nod2信号接头蛋白质, 肿瘤坏死因子 α, 基质金属蛋白酶 9, 半胱氨酸天冬氨酸蛋白酶 3

Abstract: Objective To investigate the relationship between the expressions of nucleotide-binding oligomerization domain-containing 2 (NOD2), matrix metalloproteinase -9 (MMP-9) and activated Caspase-3 in the fetal membranes and TNF-α level in amniotic fluid from women with premature rupture of the membranes (PROM)． Methods Thirty pregnant women with term premature rupture of the membranes (tPROM), 30 cases of preterm premature rupture of the membranes (pPROM) and 30 cases of healthy term pregnancy (control group) were enrolled in the experiment. Immunohistochemistry was used to detect the location of NOD2, MMP-9 and activated Caspase-3 in the fetal membranes. Western blot assay was performed to analyze the expression levels of the three proteins. ELISA was used to detect the level of TNF-α in amniotic fluid. The relationship between the levels of the four proteins was also analyzed. Results The expression levels of NOD2, MMP-9 and activated Caspase-3 in fetal membranes and the level of TNF-α in amniotic fluid were significantly higher in PROM groups than those in control group (P＜0.05). The levels of NOD2 and TNF-α were significantly higher in pPROM group than those in tPROM group (P＜0.05). There were no significant difference in the levels of MMP-9 and activated Caspase-3 between tPROM group and pPROM group. Furthermore, the level of NOD2 was positively correlated with the levels of TNF-α, MMP-9 and activated Caspase-3 (P＜0.01). Conclusion The elevated expression levels of NOD2, TNF- α, MMP-9 and activated Caspase-3 play an important role in PROM. NOD2, TNF-α, MMP-9 and activated Caspase-3 may play associative roles in the process of PROM.

Key words: fetal membranes, premature rupture, Nod2 signaling adaptor protein, tumor necrosis factor-alpha, matrix metalloproteinase 9, Caspase 3