天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (4): 271-274.doi: 10.11958/20193097

• 实验研究 • 上一篇    下一篇

消结安对大鼠子宫肌瘤超微结构影响的实验研究 #br#

叶娟 1，郝迪 2，王梓 2，李旭 2，孙国珍 1，贾淑杰 2△
  

  1. 1天津市中心妇产科医院（邮编 300100）；2天津市医药科学研究所
  • 收稿日期:2019-10-17 修回日期:2020-01-20 出版日期:2020-04-15 发布日期:2020-06-23
  • 通讯作者: 贾淑杰 E-mail:haod2005@126.com
  • 作者简介:叶娟（1981），女，硕士，主管药师，主要从事医院药事管理方面研究
  • 基金资助:
    天津市卫生和计划生育委员会中医中西医结合科研课题重点项目（2017065）

Experimental study on the effect of Xiaojie-An on ultrast ructure of uterine fibroids in rats

YE Juan1, HAO Di2, WANG Zi2, LI Xu2, SUN Guo-zhen1, JIA Shu-jie2△ #br#   

  1. 1 Tianjin Central Hospital of Gynecology Obstetrics, Tianjin 300100, China; 2 Tianjin Institute of
    Medical and Pharmaceutical Science
  • Received:2019-10-17 Revised:2020-01-20 Published:2020-04-15 Online:2020-06-23
  • Contact: JIA Shu-jie E-mail:haod2005@126.com

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摘要： 目的 探讨消结安对子宫肌瘤细胞超微结构变化的影响及其治疗子宫肌瘤的作用机制。方法 肌内注
射苯甲酸雌二醇注射液每周 3次，连续 10周后，每周加注黄体酮注射液 2次，连续 5周，制备子宫肌瘤大鼠模型，模型
制备成功大鼠按体质量随机分为模型组，消结安高、中、低剂量组，阳性药组，正常组，每组 8只。消结安灌胃给药 4
周，观察消结安对病变大鼠子宫大体形态、血清雌激素、孕激素的变化，测定子宫颈以上横径，左侧分角子宫根部起
的子宫直径，电镜观察子宫细胞超微结构的改变。结果 建模后，模型组子宫系数、分角直径和分角根部直径均大
于正常组（P＜0.05）。阳性药组、消结安高、中、低剂量组子宫系数、分角直径、分角根部直径均小于模型组（P＜
0.05），消结安各剂量组间差异无统计学意义。透射电镜下观察显示，消结安能有效地改善子宫肌瘤大鼠子宫的超微
结构病变，抑制平滑肌细胞的异常增殖。病理检查显示，对照组子宫内膜呈分泌期变化，间质可见不同程度的嗜酸
性白细胞浸润，肌层未见异常。模型组子宫内膜呈分泌期变化，子宫平滑肌层增厚，肌细胞数量增多肥大，排列欠规
整，少量中性粒细胞浸润。高、中、低剂量组和阳性药组与模型组比较均有一定改善作用，子宫平滑肌层变薄，结构
尚清楚，肌细胞基本恢复常态；但肌层见局部增厚，肌细胞数量增多肥大，排列欠规整。以高剂量组和阳性对照组为
佳。结论 消结安可使子宫肌瘤大鼠子宫体积缩小，改善子宫肌瘤细胞的超微结构，使其接近正常细胞结构，进而
达到治疗作用。

关键词: 平滑肌瘤, 子宫肿瘤, 显微镜检查, 电子, 扫描透射, 性腺甾类激素, 消结安

Abstract: Objective To investigate the effect of Xiaojie-An on ultrastructural changes of uterine flesh tumour cells
and reveal the possible mechanism for the treatment of uterine fibroids. Methods The rat model of hysteromyoma was
established by intramuscular injection of estradiol benzoate three times a week for five weeks. The model rats were randomly
divided into 5 groups according to their body weights including model group, Xiaojie-An groups (high, medium and low
doses), positive drug group and control group. After treatment for 4 weeks, the changes of uterine morphology, serum estrogen
and progesterone were observed, and the diameter of uterus above cervix, cervical transverse diameter of uterus from the root
of left angular uterus were measured. The ultrastructure changes of uterine cells were observed under electron microscope.
Results The uterine coefficient, ovarian coefficient, angular diameter and root diameter of angular fraction were all bigger
in the model group than those in the control group (P＜0.05). The uterine coefficient, horn diameter and root diameter were
all smaller in the positive drug group than those of Xiaojie-An groups (high, medium and low doses, P＜0.05). There were no
significant difference in above indexes between (high, medium and low dose Xiaojie-An groups. Results of transmission
electron microscopy showed that the treatment with Xiaojie-An could effectively improve the ultrastructural changes of
uterine fibroids and inhibit the abnormal proliferation of smooth muscle cells. Pathological examination showed that the
secretory changes in endometrium of the control group, with varying degrees of eosinophilic leukocyte infiltration in the
interstitium and no abnormalities in the muscular layer. In the model group, the endometrium showed secretory changes, the
smooth muscle layer of the uterus was thickened, the number of muscle cells increased, hypertrophy, irregular arrangement and small amount of neutrophils infiltration. High dose group and positive drug control group showed the better improvement.
Conclusion Xiaojie-An can reduce the volume of uterus, improve the ultrastructure of myoma cells, and then achieve the
therapeutic effect.

Key words: leiomyoma, uterine neoplasms, microscopy, electron, scanning transmission, gonadal steroid hormones,
Xiaojie-An

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