天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (7): 739-741.doi: 10.11958/j.issn.0253-9896.2015.07.010

• 实验研究 • 上一篇    下一篇

中央内侧丘脑核中 NMDA 受体参与丙泊酚致意识消失的作用

张益，罗竺欣，王袁，段自坤，李佳   

  1. 1贵州省遵义医学院附属医院麻醉科 （邮编 563000）； 2遵义医学院麻醉与器官保护基础研究重点实验室
  • 收稿日期:2015-01-29 修回日期:2015-03-18 出版日期:2015-07-15 发布日期:2015-07-15
  • 通讯作者: 张益 E-mail:cherishher1998@126.com
  • 作者简介:张益 （1983）， 男， 主治医师， 博士在读， 主要从事全身麻醉机制研究
  • 基金资助:
    贵州省教育厅项目 （黔科合 J字 LKZ[2011]06号）

NMDA receptors in central medial thalamus participate in propofol-induced unconsciousness

ZHANG Yi, LUO Zhuxin, WANG Yuan, DUAN Zikun, LI Jia   

  1. 1 Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Guizhou 563000, China; 2 Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical University
  • Received:2015-01-29 Revised:2015-03-18 Published:2015-07-15 Online:2015-07-15
  • Contact: Zhang Yi E-mail:cherishher1998@126.com

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摘要： 目的 探讨中央内侧丘脑核（CMT）中 N-甲基-D-天门冬氨酸（NMDA）受体在全身麻醉意识消失中的作用。方法 60 只大鼠微注射模型随机均分为 4 组： NMDA10 mmol/L 组、 NMDA20 mmol/L 组、 NMDA40 mmol/L 组及对照组 （C 组）， 丙泊酚麻醉后分别于前 3 组 CMT 内微量泵注 10、 20 和 40 mmol/L 的 NMDA 以及等体积生理盐水 （C组）。观察微注射后自主体动反应的发生率以及翻正反射恢复时间， 并通过组织学对微注射位点进行定位。结果 当注射位点在 CMT 中时， 与 C 组相比， 各浓度 NMDA 组大鼠麻醉后翻正反射恢复时间均明显缩短； 其中 NMDA20 mmol/L 与 40 mmol/L 组恢复时间短于 NMDA10 mmol/L 组， 且 NMDA20 mmol/L 与 40 mmol/L 组大鼠麻醉中自主体动的发生率显著高于 C 组 （均 P < 0.05）。当注射部位在 CMT 外时， 各浓度 NMDA 组翻正反射恢复时间均明显长于注射部位在 CMT 内者（P < 0.05）， 而各组间比较自主体动发生率与翻正反射恢复时间均无明显差异（P＞0.05）。结论 CMT 中的NMDA 受体参与了丙泊酚所导致的全身麻醉效应。
 

关键词: 麻醉药,全身, 丘脑中央核, 受体, N-甲基-D-天冬氨酸, 二异丙酚, 中央内侧丘脑, NMDA 受体, 意识消失, 脑区微注射

Abstract: Objective To investigate the role of NMDA receptors in central medial thalamus (CMT) in the unconsciousness induced by general anesthesia. Methods A total of 60 rat models for microinfusion were assigned into 4 groups (n=15 for each group). After induction with propofol, 10 mmol/L (NMDA10 group), 20 mmol/L (NMDA 20 group) and 40 mmol/L (NMDA40 group) of NMDA and normal saline (group C) with equal volume were microinfused into CMT. The incidence of purposeful movement and recovery time of righting reflex were observed in each group respectively. Infusion sites were localized by histological method. Results When the microinfusion site localized within CMT, comparing with group C, the recovery time of righting reflex reduced notably in three NMDA groups (P < 0.05). The recovery time was significantly shorter in NMDA20 group and NMDA40 group than that of NMDA10 group. The incidence of purposeful movement during propofol anesthesia was higher in NMDA20 group and NMDA40 group than that of group C (P < 0.05). When the microinfusion site localized out of CMT, the recovery time of righting reflex was remarkably longer than that within CMT in three NMDA groups (P < 0.05), and there was no significant difference in the incidence of purposeful movement and recovery time between four group (P＞0.05). Conclusion Microinfusion of NMDA agonist into CMT reverses propofol anesthesia, indicating that NMDA receptor in CMT may contribute to the propofol-induced unconsciousness.

Key words: anesthetics, general, midline thalamic nuclei, receptors, N-methyl-D-aspartate, propofol, central thalamus, NMDA receptor, loss of consciousness, intracranial microinjection