天津医药 ›› 2015, Vol. 43 ›› Issue (7): 735-738.doi: 10.11958/j.issn.0253-9896.2015.07.009

• 实验研究 • 上一篇    下一篇

糖肾方对 2 型糖尿病大鼠肝脏氧化应激水平及病理改变的影响

李忻,顾立刚,赵婷婷,杨鑫,张韫,张浩军,严美花,董晞,赵海玲,文玉敏,李平   

  1. 1 北京中医药大学 (邮编 100029); 2 中日友好医院
  • 收稿日期:2015-02-13 修回日期:2015-03-20 出版日期:2015-07-15 发布日期:2015-07-15
  • 通讯作者: 李平 E-mail: lp8675@163.com E-mail:13552698576@163.com
  • 作者简介:李忻(1980), 女, 助理研究员, 博士在读, 主要从事中西医结合基础研究
  • 基金资助:
    国家自然科学基金资助项目(81173422); 国家国际科技合作专项课题 (2011DFA31860

Effects of Tangshen Formula on liver oxidative stress and pathologic changes in typeⅡdiabetic rats

LI Xin, GU Ligang, ZHAO Tingting, YANG Xin, ZHANG Yun, ZHANG Haojun, YAN Meihua, DONG Xi, ZHAO Hailing, WEN YuminLI Ping   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029, China; 2 China-Japan Friendship Hospital
  • Received:2015-02-13 Revised:2015-03-20 Published:2015-07-15 Online:2015-07-15
  • Contact: LI Ping E-mail: lp8675@163.com E-mail:13552698576@163.com

摘要: 目的 观察糖肾方对 2 型糖尿病大鼠肝脏氧化应激水平及病理改变的影响, 并探讨其可能的机制。 采用高脂饲料喂养和小剂量链脲佐菌素(STZ)腹腔内注射方法, 建立 2 型糖尿病合并非酒精性脂肪肝动物模型。将造模成功的大鼠随机分为正常组、 模型组、 糖肾方组和二甲双胍组, 比较各组大鼠血清丙氨酸转氨酶(ALT)、 天冬氨酸转氨酶(AST)、 三酰甘油 (TG)、 总胆固醇 (CHO) 和低密度脂蛋白胆固醇 (LDL-C) 水平, 肝组织超氧化物歧化酶(SOD)、 过氧化氢酶(CAT)、 维生素 E VE)、 一氧化氮 (NO) 和一氧化氮合酶 (NOS) 水平, 并在光镜下观察各组大鼠肝组织病理形态学改变。结果 与模型组相比, 二甲双胍组和糖肾方组大鼠表现状态较好, 毛色较光泽, 粪便成形; 二甲双胍组和糖肾方组大鼠的肝脏质量和肝脏质量指数较模型组明显下降, 2 组大鼠血清 ALTASTTGCHOLDL-C 平显著降低。与模型组相比, 糖肾方组大鼠肝组织匀浆中 SODCATVE 水平明显升高, NONOS 水平减低; 二甲双胍组大鼠肝组织匀浆中 SODCAT 水平明显升高, NONOS 水平减低。HE 染色显示二甲双胍组和糖肾方组较模型组大鼠肝细胞脂肪变性明显减少, 肝细胞排列基本规则。结论 糖肾方可改善 2 型糖尿病大鼠肝功能, 降低血脂水平, 减轻肝脏脂质沉积, 其作用机制可能与其清除脂质过氧化物, 改善肝组织氧化应激水平有关。
 

关键词: 糖肾方, 糖尿病, 2 型, 肝损伤, 氧化性应激

Abstract: Objective To investigate the effects of Tangshen Formula on liver oxidative stress in diabetic rats, and their mechanisms thereofMethods Rat model of type 2 diabetes mellitus and nonalcoholic fatty liver disease was established by intraperitoneally injecting small dose of chain urea with cephalosporins (STZ) and feeding high fat fodderThe model rats were randomly divided into control group, model group, Tangshen Formula group and metformin groupThe serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), cholesterol (CHO), low density lipoprotein-cholesterol(LDL-C), and superoxide dismutase (SOD), catalase(CAT), vitamin E(VE), nitric oxide (NO) and nitric oxide synthase (NOS) in liver were compared between four groups. Changes of pathological morphology were observed under light microscopy. Results There were significantly decrease in serum levels of ALT, AST, TG, CHO, LDL-C in Tangshen Formula group and metformin group compared with those of model group. There were significantly higher levels of SOD, CAT and VE, and lower levels of NO and NOS of liver homogenate in Tangshen Formula group than those of model group. There were higher levels of SOD and CAT, and lower levels of NO and NOS in liver homogenate in metformin group than those of model group. HE staining showed that liver fatty degeneration was significantly reduced in metformin group and Tangshen Formula group compared with that of model group. Conclusion The fatty liver in type 2 diabetic rats is significantly improved by Tangshen Formula treatment, which is probably associated with the regulation of the response level to oxidative stress in liver

Key words: Tangshen Formula, Type 2 diabetes, Liver injury, Oxidative stress