天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (7): 759-763.doi: 10.11958/j.issn.0253-9896.2015.07.016

• 临床研究 • 上一篇    下一篇

RhoA 和 Snail 在涎腺腺样囊性癌中的表达及意义

胡瑞利，安峰，林媛媛，马赛，郭博伟   

  1. 1 河北北方学院（邮编 075000）；2河北北方学院附属第一医院
  • 收稿日期:2014-11-03 修回日期:2015-02-06 出版日期:2015-07-15 发布日期:2015-07-15
  • 通讯作者: 通讯作者 安峰 E-mail： kq126@126.com E-mail:344395129@qq.com
  • 作者简介: 胡瑞利（1986）， 女， 硕士在读,主要从事口腔颌面头颈部肿瘤研究

Significance of RhoA and Snail expression in salivary adenoid cystic carcinoma

HU Ruili, AN Feng,  LIN Yuanyuan, MA Sai, GUO Bowei   

  1. 1 Hebei North University, Zhangjiakou 075000, China；2 The First Affiliated Hospital of Hebei North University
  • Received:2014-11-03 Revised:2015-02-06 Published:2015-07-15 Online:2015-07-15
  • Contact: △Corresponding Author AN Feng E-mail: kq126@126.com E-mail:344395129@qq.com

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摘要： 摘要： 目的 探讨 RhoA 和 Snail 在涎腺腺样囊性癌（SACC）中的表达及其与癌症侵袭转移的关系。 方法 采用免疫组织化学方法检测 RhoA 和 Snail 在 55 例 SACC（SACC 组）与 20 例癌旁正常组织（对照组）中的表达情况， 分析 RhoA 和 Snail 的表达与 SACC 临床病理特征的关系及其在 SACC 组织中表达的相关性。 结果 SACC 组的 RhoA （69.1% vs 5.0%）和 Snail（72.7% vs 10.0%）蛋白阳性表达率高于对照组（ 均 P < 0.05）； 有淋巴结转移者的 RhoA 和 Snail 阳性表达率高于无转移者， Ⅲ+Ⅳ期的 RhoA 和 Snail 阳性表达率高于Ⅰ +Ⅱ 期者；实体型的 RhoA 阳性表达率高于筛孔型， 实体型和管状型的 Snail 阳性表达率高于筛孔型（均 P < 0.05）， 而不同性别、年龄及肿瘤部位的 RhoA 和 Snail 阳性表达率差异无统计学意义； RhoA 和 Snail 在 SACC 中的表达呈正相关（rs=0.414， P < 0.001）。 结论 RhoA 和 Snail 蛋白可能通过 RhoA/ROCK/PKD1/NF-κB/Snail 信号传导通路联合作用促进了 SACC 的浸润和转移。

关键词: 涎腺肿瘤, 癌, rho相关激酶类, 肿瘤浸润, 肿瘤转移, RhoA, Snail, 涎腺腺样囊性癌

Abstract: Abstract: Objective To investigate the relationship of RhoA and Snail expressions, and the invasion and metastasis in salivary adenoid cystic carcinoma (SACC). Methods The expressions of RhoA protein and Snail protein in 55 samples of SACC (SACC group ) and 20 samples of para-carcinoma normal tissues(control group) were detected using immunohisto⁃ chemical method. The relationship between RhoA protein and Snail protein expressions and clinical and pathological charac⁃ teristics were analyzed. Results The positive expressions of RhoA protein (69.1% vs 5.0%) and Snail protein (72.7% vs 10.0%) were significantly higher in SACC group than those in control group (P < 0.05). The positive expression rates of RhoA protein and Snail protein were significantly higher in patients with lymph node metastasis than those in patients with⁃ out lymph node metastasis. The positive expression rates of RhoA protein and Snail protein were significantly higher in pa⁃ tients at Ⅲ +Ⅳ stage than those in patients at Ⅰ +Ⅱ stage. The positive expression rates of RhoA protein and Snail protein were significantly higher in substantive carcinal tissues than those in screen roller type and tubular carcinal tissues. The posi⁃ tive expression of Snail protein was significantly higher in substantive and tubular carcinal tissues than that in screen roller type carcinal tissues (P < 0.05). There were no significant differences in positive expression rates of RhoA and Snail between different gender, age and different carcinal tissues. There was a positive correlation beween expression rates of RhoA and Snail protein in SACC (r=0.414, P < 0.001). Conclusion RhoA and Snail may both facilitate the infiltration and metastasis of SACC through RhoA/ROCK/PKD1/NF-kappa B/Snail signaling pathways