天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (9): 996-1000.doi: 10.11958/j.issn.0253-9896.2015.09.0010

• 临床研究 • 上一篇    下一篇

上皮源性卵巢中4种miRNAs的表达及其临床意义

刘佳宇1,2， 赵妍蕊1， 张丽娜1,3， 闫晔4， 郑红1△   

  1. 作者单位： 1天津医科大学肿瘤医院， 肿瘤研究所肿瘤分子流行病与生物统计研究室， 国家肿瘤临床研究中心， 天津市肿瘤防治重点实验室 （邮编300060）； 2天津医科大学研究生院； 3天津医科大学肿瘤医院乳腺外科； 4天津医科大学总医院妇产科
  • 收稿日期:2015-04-28 修回日期:2015-05-07 出版日期:2015-09-15 发布日期:2015-09-15
  • 通讯作者: 郑红 E-mail: zhengh64@aliyun.com E-mail:zhengh64@aliyun.com
  • 作者简介: 刘佳宇(1989)， 女， 硕士在读， 主要从事肿瘤分子流行病学研究
  • 基金资助:
    国家自然科学基金资助项目 （81072363）

Expression and clinical significance of four miRNAs in epithelial ovarian cancer

LIU Jiayu 1,2, ZHAO Yanrui1, ZHANG Lina1,3, YAN Ye4, ZHENG Hong1△   

  1. 1 Department of Epidemiology and Biostatistics, Tianjin Medical University Cancer Hospital and Institute, National Clinical Research Center of Cancer, Key Laboratory of Breast Cancer Prevention and Therapy， Tianjin 300060, China;2 Gradute School，Tianjin Medical University ;3 Department of Breast Surgery, Tianjin Medical University Cancer Hospital and Institute；4 Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital
  • Received:2015-04-28 Revised:2015-05-07 Published:2015-09-15 Online:2015-09-15
  • Contact: Hong ZHENG E-mail: zhengh64@aliyun.com E-mail:zhengh64@aliyun.com

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摘要： 目的 分析上皮源性卵巢癌 （EOC） 组织中 miR-200a、 miR-141、 miR-205 和 miR-34a 的表达及其临床意义。方法 44 例 EOC 患者按 FIGO 分期分为 FIGO Ⅰ~Ⅱ （FIGO 早期组） 组 15 例和 FIGO Ⅲ~Ⅳ （FIGO 晚期组） 组 29 例， 用实时荧光定量 PCR 定量比较 2 组 4 种 miRNAs 的表达差异并考察 4 种 miRNAs 表达间的关联。以各组 miRNAs 表达的中位数将 EOC 患者分为 miRNA 高表达组和低表达组， 比较 2 组患者间的生存差异。按年龄、 FIGO 分期、 术后是否有残余瘤及术后化疗情况分组， 进行 Kaplan-Meier 生存分析和 Cox 多因素分析。结果 FIGO 晚期组 miR-141 的表达高于早期组 （P = 0.036）。miR-141 的表达与 miR-200a、 miR-205 呈正相关， 与 miR-34a 呈负相关 （均P < 0.05）； miR-200a与miR-205的表达呈正相关 （P < 0.05）。生存分析显示， miR-200a的表达低， 卵巢癌患者无进展生存期短（P = 0.035）， FIGO早期组生存率高于FIGO晚期组 （P< 0.05）。miR-200a的表达水平、 FIGO分期以及年龄与卵巢癌患者总生存期和无进展生存期有关， miR-141、 miR-205和miR-34a的表达水平、 术后残余瘤以及化疗与患者生存无关。结论 miR-200a的表达与卵巢癌患者预后有关， 可能是卵巢癌患者预后预测的独立影响指标。

关键词: 微RNAs, 卵巢肿瘤, 上皮-间质转化, 上皮源性, miR-200a, miR-141, miR-205, miR-34a

Abstract: Objective To determine the expressions of miR- 200a, miR-141, miR-205 and miR-34a in epithelial ovarian cancer (EOC) samples and to explore their clinical significance. Methods According to FIGO staging, 44 EOC pa⁃ tients were divided into two groups: early FIGO stage (stage Ⅰ-Ⅱ, n=15) and late FIGO stage (stage Ⅲ-Ⅳ, n=29). Expres⁃ sions of 4 miRNAs were detected by real time quantitative PCR, and were compared between two groups. The correlation of 4 miRNAs was calculated. EOC patients were divided into high miRNA expression group and low expression group according to the median value of miRNAs expression. Kaplan-Meier survival analysis and Cox multivariate analysis were used to com⁃ pare the age, FIGO state, tumor residual after operation and post-operative chemotherapy of ovarian cancer between two groups. Results The expression of miR-141 was elevated in stages Ⅲand Ⅳ compared with that of stagesⅠand Ⅱ(P = 0.036). There was a positive correlation between expression of miR-141, miR-200a and miR-205, but a negative correlation with miR-34a (P< 0.05). There was a positive correlation between miR-200a and miR-205 (P < 0.05). Lower miR-200a ex⁃ pression was associated with shorter progress free survival in ovarian cancer analyzed by log-rank test (P = 0.035). The sur⁃ vival rate was significantly higher in FIGO stages Ⅰ and Ⅱ than that of FIGO stages Ⅲ and Ⅳ(P < 0.05). Cox regression analysis revealed that miR-200a, FIGO stage and age were influential factors of overall survival time and progress-free sur⁃ vival time of ovarian cancer, while miR-141, miR-205, miR-34a and tumor residual after operation and post-operative che⁃ motherapy were not influential factors. Conclusion The expression of miR-200a is closely correlated with the progress and prognosis of ovarian cancer and may be used as an independent indicator for ovarian cancer prognosis.

Key words: microRNAs, ovarian neoplasms, epithelial-mesenchymal transition, epithelial, miR- 200a, miR- 141, miR-205, miR-34a