天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (1): 34-37.doi: 10.3969/j.issn.0253-9896.2015.01.009

• 细胞与分子生物学 • 上一篇    下一篇

PTEN 调节神经样细胞微管相关蛋白 tau 的聚集及意义

闫旭 1,2， 阚世廉 2△， 金晔 3#br# #br#   

  1. 1 天津医科大学研究生院（邮编 300070）；2天津市天津医院骨科；3 中国医学科学院北京协和医学院北京协和医院中心实验室
  • 收稿日期:2014-06-09 修回日期:2014-08-29 出版日期:2015-01-15 发布日期:2015-01-30
  • 通讯作者: 阚世廉 ，△ 通讯作者 E-mail： pumc@sina.com E-mail:pumc@sina.com

Aggregation of microtubule-associated protein tau modulated by PTEN during the differentiation of bone marrow stem cell-derived nerve cells

YAN Xu1， 2, KAN Shilian2△, JIN Ye3   

  1. 1 Graduate School, Tianjin Medical University， Tianjin 300070, China； 2 Tianjin Hospital； 3 Central Laboratory, Peking Union Medical College Hospital
  • Received:2014-06-09 Revised:2014-08-29 Published:2015-01-15 Online:2015-01-30
  • Contact: KAN Shilian, △Corresponding Author E-mail： pumc@sina.com E-mail:pumc@sina.com

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摘要： 摘要： 目的 观察体外诱导成人骨髓基质干细胞（BMSC）向神经细胞分化过程中， 在第 10 号染色体缺失的磷酸酶及张力蛋白同源物基因（PTEN）调节细胞骨架中轴突标志分子微管相关蛋白 tau（MAPT）分布及聚集的特征， 并探讨其意义。 方法 从成人骨髓中分离基质干细胞， 在体外经细胞因子诱导分化 2 周后成为神经样细胞， 将未分化的 BMSC 作为对照组； 应用半定量逆转录-聚合酶链反应（RT-PCR）和 Western blot 方法研究 MAPT 的表达变化； 经 PTEN 抑制剂 BPV 作用于该细胞后， 用结合有荧光剂的鬼笔环肽直接染色细胞微丝肌动蛋白， 并联合用免疫荧光细胞化学方法染色 MAPT， 在激光共聚焦显微镜下观察 MAPT 和肌动蛋白的定位及关联性。 结果 成人骨髓来源的基质干细胞经诱导分化后， MAPT 的 mRNA 在分化 1 周和 2 周时相对表达水平为 0.24±0.04 和 0.52±0.04， 高于对照组 BMSC 的 0.04±0.02（P＜ 0.05）。 MAPT 蛋白表达水平分别为 0.18±0.03 和 0.44±0.05， 高于对照组 0.06±0.04（P＜ 0.05）。 细胞染色后， 经 BPV 作用后 MAPT 由弥散状态变为聚集状态， 并分布于细胞一侧， 神经样细胞开始出现极性。 结论 BMSC 来源的神经细胞分化成熟时， PTEN 参与调节细胞骨架中轴突标志分子 MAPT 的转运和聚集， 为神经细胞的轴突进一步生长提供物质支持。

关键词: 神经细胞, 细胞分化, 第 10 号染色体缺失的磷酸酶及张力蛋白同源物基因, 微管相关蛋白 tau, 细胞骨架

Abstract: Abstract： Objective To observe the diffusion and aggregation of the microtubule associated protein tau（MAPT）modu⁃ lated by phosphatase and tensin homolog deleted on chromosome ten（PTEN）during the nerve cell differentiation by human bone marrow stem cells（BMSC）in vitro, and to analyse the signification. Methods Adult bone marrow stem cells were iso⁃ lated and induced into nerve-like cells by some cytokines in vitro. The mRNA expression of MAPT was detected by semiquantitative RT-PCR and Western blot assay. The patterns of diffusion and aggregation of the MAPT association of the actin were indicated by Phalloidin-fluoresceineisothioeyanate (FITC) and immunofluorescence (IF) cyto-chemistry, and observed by the laser-confocal microscopy. Results The MAPT mRNA levels were 0.24 ± 0.04 and 0.52 ± 0.04 at 1 week and 2 weeks after the induction， which were significantly higher compared with those of BMSC (0.04 ± 0.02) after the induction (P＜ 0.05). The MAPT protein levels were 0.18 ± 0.03 and 0.44 ± 0.05 at 1 week and 2 weeks after the induction, which were significantly higher compared those of BMSC (0.06 ± 0.04, P＜ 0.05). The distribution patterns of MAPT were changed from the diffusion to the aggregation in cells after treatment by BPV. The nerve-like cells appeared the characteristic of po⁃ larization. Conclusion When the nerve cells derived from bone marrow stem cells obtain the mature differentiation, PTEN may possess the ability of modulating the diffusion and aggregation of MAPT in vitro, also may provide a kind of material ba⁃ sis for the growth of the nerve axon.

Key words: tumor suppressor proteins； tau proteins； cytoskeleton； reverse transcriptase polymerase chain reaction； fluo? roimmunoassay, phosphatase and tensin homolog deleted in chronlosome 10