天津医药

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不同浓度乙醇对大鼠肺组织TGFβ-1和bFGF表达的影响

华静娜1,吴琦1,武俊平2,胡金苗1,孙洪芬1   

  1. 1. 天津市海河医院
    2. 天津市海河医院呼吸科
  • 收稿日期:2010-03-16 修回日期:2010-08-16 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 吴琦

The effect of different concentrations of alcoholon the expression of TGFβ-1 and bFGF in rats’lung tissue

  • Received:2010-03-16 Revised:2010-08-16 Published:2010-12-15 Online:2010-12-15

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摘要： 目的:探讨不同浓度乙醇对大鼠肺组织中转化生长因子(TGF)-β1和碱性成纤维细胞生长因子(bFGF)表达的影响及其与肺纤维化之间的关系。方法:雄性Wistar大鼠32只随机分成对照(C)组、3%(A3)、10%(A10)和20%乙醇(A20)组,每组8只,A3、A10、A20饮入不同浓度的白酒,C组自由饮水。16周后免疫组化法检测TGF-β1和bFGF在大鼠肺组织中的表达,通过Masson染色观察肺间质中胶原沉积的情况。结果:(1)TGF-β1的阳性细胞数在乙醇组中增高;对照组与A20组、A10组间差别均有统计学意义(P<0.05),但与A3组比较差别无统计学意义(P>0.05)。(2)bFGF的阳性细胞数在乙醇组中增高;随着乙醇浓度增高阳性细胞数增加,A3、A10、A20组间差异均有统计学意义(P>0.05)。Masson结果示乙醇组较对照组在肺泡间隔中胶原纤维沉积增多,以A20组最为明显。结论:肺泡Ⅱ型上皮细胞和巨噬细胞中TGF-β1、bFGF的表达随乙醇浓度的升高而增多,且一定浓度的乙醇会引起肺组织胶原沉积,提示乙醇可能是导致肺纤维化的原因之一。 

关键词: 酒精, 肺纤维化, 转化生长因子β1, 碱性成纤维细胞生长因子, 胶原

Abstract: Objective:To observe the effects of different concentrations of alcohol intake and the expression of TGF-β1 and bFGF on pulmonary in rats, and to explore the relationship between pulmonary fibrosis and possible mechanisms. Methods: Clean Wistar male rats (n=32) were randomly divided into control group (C, n=8), free drinking water; alcohol group (A, n=24), according to different concentrations were divided into 3% alcohol group (A3, n=8), 10% alcohol group(A10,n=8), 20% alcohol group(A20,n=8). A certain concentration of liquor was drunk freely by the rats of alcohol group, with no other liquid feed. Animals of each group were put to death 16 weeks after anesthesia. The transforming growth factor β1 and the basic fibroblast Growth factor in the rat lung tissue were measured through immunohistochemistry. By Masson staining, the interstitial lung collagen deposition in the case was observed. Results: Immunohistochemical results showed: (1) the positive cells of TGF-β1: The difference between control and alcohol group is significant (P < 0.05). To compare the C with the A20, 10 groups, the differences are statistically significant (P < 0.05). But to compare with the A3 group, the difference is not significant (P > 0.05). (2)The positive cells of bFGF: The differences between control and alcohol group is significant (P < 0.05), and the differences between the alcohol group of different concentration are also significant (P < 0.05). Masson results showed the deposition of collagen in the alveolar interval increased more significantly in alcohol group than the control group, especially in the A10 and A20. Conclusions: Our study proves that the expression of TGF-β1 and bFGF increase in the alveolar type Ⅱ epithelial cells and macrophages as the increase in the concentration of alcohol. A certain concentration of alcohol would cause lung collagen deposition. Our study also suggests that alcohol may be one of the reasons for leading to pulmonary fibrosis.

Key words: alcohol, pulmonary fibrosis, transforming growth factor β1, basic fibroblast growth factor, collagen