天津医药

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HPV感染的宫颈病变对血清锌、IL-2、IL-10及组织E2F1的表达影响

辛乐1,米淑玲2,尹利荣3,马洪达1,孙俊杰4,王宇全1   

  1. 1. 天津医科大学第二医院
    2. 天津医大二院妇产科
    3. 天津医科大学第二医院妇科
    4. 天津医大二院妇科
  • 收稿日期:2010-07-30 修回日期:2010-09-04 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 辛乐

Study on the expression and the significance of zn and IL-2、IL-10 in serum and E2F1 in organization of all kinds cervical diseases patients’ with high-risk HPV

  • Received:2010-07-30 Revised:2010-09-04 Published:2011-01-15 Online:2011-01-15

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摘要： 摘要 目的：探讨高危型人乳头状瘤病毒(HPV)感染的宫颈病变患者血清锌、白细胞介素（IL）-2及IL-10的含量及组织中E2F1的水平及其临床意义。方法 :选取宫颈上皮内瘤样病变（CIN）I级组、CINII级组各30例、CINIII级及宫颈癌组各20例，均为高危型HPV感染。慢性宫颈炎患者30例,对照组HPV（-）。分别用原子吸收光谱法测定血清锌，酶联免疫吸附法(ELISA)测定IL-2及IL-10的含量，并用免疫组化法测定宫颈组织中E2F1表达。结果:（1）血清锌、血清IL-2的含量在CINⅡ级组、CIN Ⅲ级组、宫颈癌组中逐级降低，3组间比较差异均有统计学意义，以上3组分别与CINⅠ级、对照组比较差异亦均有统计学意义（均P＜0.01）。宫颈癌组血清IL-10高于CIN各级别组，CIN Ⅲ级组高于Ⅱ级组，差异均有统计学意义（P＜0.05或P＜0.01）。组织中E2F1的表达在宫颈癌组、CIN组及对照组间比较差异有统计学意义（P<0.005），CIN各组内相比差异无统计学意义（P>0.005）。（2）血清IL-2与锌水平呈正相关（P<0.05〉。血清IL-2与IL-10水平、组织E2F1表达均呈负相关（P<0.05 ）。结论:高级别宫颈病变及宫颈癌患者中存在Th1/Th2的漂移，可能影响了机体对HPV的清除。机体缺锌可能参与了这种变化。

关键词: 癌, 锌, 白细胞介素 2, 白细胞介素 10, E2F1 转录因子 乳头状瘤病毒科, 宫颈上皮内瘤样病变, 宫颈肿瘤

Abstract: Abstract：Through objective the zinc 、 IL-2、IL-10 content in serum and E2F1 in organization of cervical disease patients with the high-risk HPV infection,investigate it’s Clinical significance.Methods :Selected 130 cervical disease patients , including 30 CIN Ⅰ、30 CIN Ⅱ、20CIN Ⅲ and 20 cervical carcinoma with high-risk HPV infection, 30chronic cervicitis with HPV infection negetive. Detect Zn by Atomic absorption spectrometry and detected IL-2、IL-10 in their serum by ELISA. The expressions of E2F1 were detected by immunohistochemistry. Results:The content of Serum zinc、IL-2，in the CIN Ⅱ、CIN Ⅲand cervical cancer were decrease were all significantly(p<0.05);the content of IL-10，in the CIN Ⅲand cervical cancer were decrease were all significantly(p<0.05); the compare of the expression of E2F1 ，in the chronic cervicitis、the CIN and cervical cancer were all significantly(p<0.005).IL-2’s levels and zinc’s in serum was positively correlated（p<0.05), ,IL-2’s levels and IL-10 in serum was negatively correlated（p<0.05）, IL-2’s levels and E2F1’s in serum was negatively correlated（p<0.05 ）. Conclusion:In the high-level cervical disease and cervical cancer , occurred the drift of Thl to Th2, impact the clear of HPV . Zinc deficiency involved in this change. .

Key words: carcinoma, zinc, interleukin-2, interleukin-10, E2F1 transcription factor papillomavirideae, cervical intraepithelial neoplasia, uterine cervical neoplasms