天津医药

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慢性间歇性低压低氧对丹皮酚抑制大鼠回肠收缩活动的影响

宋爽1,宋士军2,张翼2,于雷3,赵晗洁3,吴婵3   

  1. 1. 河北医科大学七年制临床医学专业2008级
    2. 河北医科大学生理学教研室
    3. 河北医科大学临床医学专业2008级
  • 收稿日期:2011-05-03 修回日期:2011-07-12 出版日期:2011-11-15 发布日期:2011-11-15
  • 通讯作者: 张翼

Chronic Intermittent Hypobaric Hypoxia on Inhibitory Effects of Paeonol in Ileum Spontaneous Contraction of Rats

  • Received:2011-05-03 Revised:2011-07-12 Published:2011-11-15 Online:2011-11-15

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摘要： 摘要 目的: 观察慢性间歇性低压低氧（CIHH）对丹皮酚抑制大鼠离体回肠收缩活动的影响，并研究其作用机制。方法:健康SD大鼠，雌雄不拘，随机分为正常氧（CON）组和CIHH组；CIHH大鼠给予28 d相当于海拔5 000 m高度、每日6 h的CIHH处理；CON组除了未进行CIHH外，其余条件与CIHH相同。常规制备离体回肠标本，通过离体灌流方法记录回肠的收缩活动。结果:（1）丹皮酚使CON和CIHH大鼠回肠收缩活动的幅度、频率、张力和活动力明显下降，并呈浓度依赖关系。以活动力为指标，IC50为65.13 mg/L和135.74 mg/L。（2）CIHH明显减弱了丹皮酚对大鼠离体回肠的抑制作用。（3）在CON，丹皮酚抑制了乙酰胆碱诱发的细胞内钙和外钙性收缩，抑制了库容性钙通道和高钾诱发的电压门控钙通道引起的收缩；L-NAME、吲哚美辛和心得安均可部分阻断丹皮酚对大鼠回肠平滑肌收缩活动的抑制作用。（4）在CIHH，丹皮酚未抑制乙酰胆碱诱发的细胞内钙性收缩和库容性钙通道引起的收缩，心得安也未阻断丹皮酚的抑制作用。 结论: CIHH减弱了丹皮酚对大鼠离体回肠收缩的抑制作用。其机制可能是CIHH取消了丹皮酚的抑制内钙释放、抑制库容性钙内流和激动β2受体作用。

关键词: 慢性间歇性低压低氧, 丹皮酚, 大鼠, 回肠, 一氧化氮

Abstract: Abstract Objective: To investigate the effect of chronic intermittent hypobaric hypoxia (CIHH) on spontaneous contraction of rat ileum inhibited by paeonol in vitro, and the mechanism thereof. Methods: Sprague-Dawlay (SD) rats were randomly divided into normal oxygen group (CON) and CIHH treatment group (CIHH). Rats of CIHH group were exposed to hypoxia in a hypobaric chamber simulating 5000m altitude, 6 hours daily for 28 days. CON animals lived in the same environment as CIHH animals except hypoxia. Samples of rat ileum were prepared routinely. The ileum contractions were recorded using organ bath technique. Results: (1) Paeonol significantly decreased the amplitude, frequency, tension and activity of the ileam contraction in a dose-dependent manner both in CON and CIHH groups. With activity as criteria, IC50 was 65.13 mg/L and 135.74 mg/L. (2) CIHH significantly attenuated the inhibitory effects of Paeonol. (3) Paeonol inhibited acetylcholine-induced contraction of extracellular and intracellular calcium significantly in CON group. Paeonol inhibited the contraction of store-operated calcium channel (ROCC) and voltage-operated calcium channel. The inhibitory effect of Paeonol can be partly blocked by L-NAME, indomethacin and propranolol. (4) The acetylcholine-induced intracellular calcium release and ROCC-induced contraction were not blocked by Paeonol in CIHH, and which was not blocked by propranolol. Conclusion：CIHH reduced the inhibitory effects of Paeonol on rat ileum, which may be caused by the blocking of Paeonol induced ntracellular Ca2+ release, inhibiting of the ROCC and β- adrenergic receptor through CIHH.

Key words: Chronic intermittent hypobaric hypoxia, Paeonol, Rats, Ileam, Nitric oxide