天津医药

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PI3K／Akt信号转导通路与卵巢癌顺铂耐药相关性的研究

张栋1,孔德璇2,和珍珍3,瞿全新3   

  1. 1. 天津医科大学研究生院
    2. 天津市第一中心医院
    3. 天津医科大学一中心临床学院
  • 收稿日期:2010-06-07 修回日期:2010-08-19 出版日期:2011-04-15 发布日期:2011-04-15
  • 通讯作者: 瞿全新

Sudy on the Relationship between PI3K/Akt Signal Transduction Pathway and DDP Resistance of Ovarian Carcinoma

  • Received:2010-06-07 Revised:2010-08-19 Published:2011-04-15 Online:2011-04-15

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摘要： 摘要 目的：研究卵巢癌顺铂耐药与PI3K／Akt信号转导通路的关系。方法：Western Blot法检测SKOV3和SKOV3/DDP细胞PI3K、Akt表达水平及LY294002、顺铂对卵巢癌细胞中PI3K、Akt表达的影响；MTT法检测LY294002对卵巢癌细胞顺铂化疗敏感性的影响。结果：SKOV3细胞PI3K和Akt蛋白相对浓度值均低于SKOV3/DDP细胞。LY294002可降低SKOV3和SKOV3/DDP细胞中PI3K和Akt表达。SKOV3和SKOV3/DDP细胞经LY294002 40μmol/l作用后，对顺铂敏感性分别增加了67.36%、76.56%。结论：PI3K/Akt信号通路异常与卵巢癌顺铂耐药有关，抑制PI3K/Akt信号通路能增强卵巢癌顺铂敏感性。

关键词: 卵巢癌, 顺铂, 耐药, PI3K/Akt, SKOV3, SKOV3/DDP

Abstract: Abstract Objective:Study the relationship between cisplatin resistance of ovarian carcinoma and PI3K/Akt singnal transduction pathway. Methods:Western blot was used to detect the expression level of PI3K and Akt in SKOV3 and SKOV3/DDP cells.The effection of LY294002 and DDP to expression of PI3K and Akt in Ovarian carcinoma cells were detected by Western Blot. MTT assay was used to analyze the synergism of LY294002 to DDP and the effection of LY294002 to control ovarian tumor cells growth .Results:The correspondence density of PI3K and Akt in SKOV3 cells were lower than that in SKOV3/DDP cells.LY294002 could reduce the expression of PI3K and Akt in SKOV3 and SKOV3/DDP cells. After interferenced by LY294002 40μmol/l, the sensitivity of SKOV3 cells to DDP increased 67.36% and the sensitivity of SKOV3/DDP cells to DDP increased 76.56%. Conclusion:The abnomal PI3K Akt/signaling pathways related to Cisplatin resistance of Ovarian carcinoma. Inhibiting PI3K Akt/signaling pathways could reverse ovarian cisplatin drug-resistant phenotype.

Key words: Ovarian carcinoma, Cisplatin, Drug-resistance, PI3K/Akt, SKOV3, SKOV3/DDP