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糖尿病结肠动力障碍近端结肠平滑肌细胞相关凋亡基因表达的研究

孙曼怡,刘燕,冯凭   

  1. 天津医科大学总医院
  • 收稿日期:2010-07-28 修回日期:2010-12-01 出版日期:2011-05-15 发布日期:2011-05-15
  • 通讯作者: 孙曼怡

Apoptosis of Proximal Colon Smooth Muscle Cell in Diabetic Colon Dysmotility

  • Received:2010-07-28 Revised:2010-12-01 Published:2011-05-15 Online:2011-05-15
  • Contact: Man-Yi SUN

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摘要： 摘要 目的：探讨糖尿病（DM）结肠动力障碍近端结肠平滑肌细胞的凋亡变化。方法：雄性SD大鼠36只随机分为正常对照6周组、正常对照10周组、DM 6周组和DM 10周组，每组9只。检测空腹血糖（FBG）、胃肠推进率，血清胰岛素（FINS），HE染色观察近端结肠平滑肌的变化，应用荧光实时定量PCR（RT-qPCR）检测大鼠近端结肠平滑肌细胞的bax、bcl-2及caspase-3的mRNA表达情况。结果：⑴DM组大鼠较同时间点正常对照组大鼠空腹血糖显著增高，胃肠推进率及血清胰岛素显著降低，近端结肠平滑肌变薄，bax及 caspase-3的mRNA表达增强， bcl-2的mRNA表达降低，差异有统计学意义（P＜0.01）。⑵DM 10周组大鼠较DM 6周组大鼠的空腹血糖增高，胃肠推进率及血清胰岛素降低，近端结肠平滑肌变薄，bax及 caspase-3的mRNA表达增强， bcl-2的mRNA表达降低，两组比较差异有统计学意义（P＜0.05）。结论：DM结肠动力障碍与近端结肠平滑肌细胞的凋亡密切相关，且细胞凋亡随病程发展而加重。

关键词: 糖尿病, 结肠动力障碍, 平滑肌细胞, 凋亡

Abstract: Abstract Objective: To explore the alteration of apoptosis of proximal colon smooth muscle cell in diabetic colon dysmotility. Methods: Thirty-six male SD rats were randomly divided into 4 groups which included normal control 6w group、normal control 10w group、DM 6w group and DM 10w group (n=9). Fasting blood glucose(FBG)、gastrointestinal transit rate and the serum level of fasting insulin(FINS) were detected, and the changes in the smooth muscle of proximal colon were examined by HE staining. The mRNA expression levels of bax 、bcl-2 and caspase-3 in the proximal colon smooth muscle cells were determined by real-time quantitative PCR(RT-qPCR).Results: ⑴ FBG of DM group was significantly higher than that of the normal control group. Gastrointestinal transit rate and FINS of DM group were significantly lower than those of the normal control group. The smooth muscle of proximal colon in DM group became thinner than that in the normal control group. The mRNA expression levels of bax and caspase-3 in DM group were higher than those in the normal control group and the mRNA expression level of bcl-2 in DM group was lower than that in the normal control group（P＜0.01）.⑵ FBG of DM 10w group was higher than that of DM 6w group. Gastrointestinal transit rate and FINS in DM 10w group were lower than those in DM 6w group. The smooth muscle of proximal colon in DM 10w group became thinner than that in DM 6w group. The mRNA expression levels of bax and caspase-3 in DM 10w group were higher than those in DM 6w group and the mRNA expression level of bcl-2 in DM 10w group was lower than that in DM 6w group（P＜0.05）.Conclusion: Diabetic colon dysmotility is closely associated with apoptosis of proximal colon smooth muscle cell, and the apoptosis becomes more significant with disease developing.

Key words: diabetes mellitus, colon dysmotility , smooth muscle cell, apoptosis