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缬沙坦对尼古丁损伤血管内皮依赖性舒张功能的保护作用及机制

罗宏丽,肖顺林,王述蓉   

  1. 泸州医学院附属医院
  • 收稿日期:2010-05-10 修回日期:2010-08-22 出版日期:2011-05-15 发布日期:2011-05-15
  • 通讯作者: 罗宏丽

Protection valsartan on injury of endothelium-dependent relaxation induced by nicotine

  • Received:2010-05-10 Revised:2010-08-22 Published:2011-05-15 Online:2011-05-15

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摘要： 目的：观察缬沙坦对尼古丁损伤大鼠肠系膜动脉内皮依赖性舒张反应的保护作用及机制。方法：采用器官培养技术和离体血管环张力描记法，研究不同浓度的缬沙坦(0.1、1、10μmol?L-1)在有或无L-Nω硝基精氨酸甲酯(L-NAME，100μmol?L-1)或吲哚美辛（Indo，10μmol?L-1）的情况下，对培养24h的成年SD大鼠肠系膜动脉环(1 mm)内皮依赖性舒张反应的影响。结果：缬沙坦呈浓度依赖性改善尼古丁对血管内皮依赖性舒张反应的损害。低浓度时对尼古丁损伤没有明显影响，中、高浓度具有显著的保护作用(P<0.01，与尼古丁0.1mmol?L-1组比较)。而且，L-Nω-硝基精氨酸甲酯和吲哚美辛可阻断缬沙坦的保护作用。结论：缬沙坦保护血管内皮依赖性舒张功能可能与促进内皮细胞释放一氧化氮和前列环素有关。

关键词: 尼古丁, 缬沙坦, 肠系膜动脉

Abstract: OBJECTIVE: To observe the protective effects on endothelium- dependent relaxation (EDR) damage induced by nicotine in the isolated rat mesenteric arteries. METHODS: An organ culture system and artery ring tension recording method were used. The mesenteric artery rings (1mm) of adult Sprague-Dawley rats were cultured with different concentration of valsartan (0.1, 1, 10μmol?L-1) for 24 hours in presence or absence of nitric oxide synthase inhibitor Nω-nitro-L-arginine(L-NAME) and prostacyclin synthase inhibitor indomethacin. The cultured-artery rings were precontracted with noradrenalin (NE, 1μmol?L-1) and subsequently relaxed by a commulative addition of ACh (10-9-10-6mol?L-1). RESULTS:　Valsartan attenuated the inhibition induced by nicotine in concentration-dependent manner. There was no significant difference between valsartan (0.1μmol?L-1) group and nicotine group (P>0.05). But the EDR of the middle, high concentration (1, 10μmol?L-1) groups were significantly potentiated compared to the arteries cultured only with nicotine (P<0.01). Furthermore, the protetive effect of valsartan was blocked by L-NAME and indomethacin. CONCLUSION:　Valsartan prevents the vascular endothelium-dependent relaxation against elcited-nicotine injury with a mechanism related to the activation of nitric oxide and prostacyclin.

Key words: nicotine, valsartan, mesenteric artery