天津医药

• 实验研究 • 上一篇    下一篇

静磁场作用下金属离子对成骨细胞的生物学影响

戴闽1,周通华1,程细高2,熊皞2   

  1. 1. 南昌大学第一附属医院骨二科
    2.
  • 收稿日期:2011-01-12 修回日期:2011-03-24 出版日期:2011-08-15 发布日期:2011-08-15
  • 通讯作者: 周通华

Static magnetic field reduced the toxic effect of metal ions on osteoblasts

  • Received:2011-01-12 Revised:2011-03-24 Published:2011-08-15 Online:2011-08-15
  • Contact: Tong Hua ZHOU

PDF (PC)

1961

摘要： 【目的】探讨静磁场（SMF）作用下金属离子对成骨细胞毒性的影响，寻找防治假体周围骨溶解的理论依据。【方法】将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液，将金属离子Co2+、Cr3+分别与小鼠颅骨成骨细胞（MC3T3-E1）在不同的磁场强度(10 Gs、100 Gs、1000 Gs)下共培养。实验分4组：设Co2++Cr3+组(对照组)、和Co2++Cr3+分别+10 Gs、100 Gs、1000 Gs组。倒置显微镜下观察细胞生长情况及形态特征。扫描电镜检测成骨细胞表面超微结构。流式细胞术检测细胞凋亡。ELISA（酶联免疫分析）法对培养上清液ALP含量进行检测。【结果】静磁场作用下的成骨细胞呈现更加成熟的形态学特征，Co2++Cr3+分别+10 Gs、100 Gs、1000 Gs组凋亡比率明显降低；小鼠ALP Elisa试剂盒实验的A值明显上升。【结论】静磁场作用下Co2+ 、Cr3+金属离子对成骨细胞的毒性作用会明显减低，为磁场疗法防治假体周围骨溶解提供了实验依据。

关键词: 静磁场, 成骨细胞, 金属离子, 无菌性松动

Abstract: [Objective] Investigate the toxic effect of metal ion C02+,Cr3+ on osteoblast under the static magnetic field,and search a method for preventation and treatment of aseptic loosening.[Methods] CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mouse calvarial osteoblasts(MC3T3-E1) were cultured with C02+,Cr3+ ions in different magnetic field of 10Gs，100Gs，1000Gs for 12，24 and 48 h．Observe morphological features of cell growth under inverted microscope . Scanning electron microscopy detect ultrastructure of osteoblasts. Cell apoptosis rate was assured by flow cytometry. ELISA method was appied to detect ALP content in serum supernatant.[Results] Under the static magnetic field,osteoblast showed more mature morphological features; the group 10Gs，100Gs，1000Gs has obviously lower apoptosis rate compared with the expriment group; the A value tested by ALP ELISA method in expriment group up-regulated obviously. [Conclusion] The static magnetic field could reduced the toxic effect of metal ion C02+,Cr3+ on osteoblast, offered the experimental evidence for preventation and treatment of aseptic loosening with magnetic field therapy.

Key words: static magnetic field, osteoblast, metal ion, aseptic loosening