天津医药

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壬基酚对大鼠甲状腺功能影响的研究

奚悦   

  1. 辽宁医学院附属第三医院
  • 收稿日期:2010-10-09 修回日期:2011-01-08 出版日期:2011-09-15 发布日期:2011-09-15
  • 通讯作者: 奚悦

Effects of Nonylphenol on the Structure and Function of Thyroid gland in Rats

  • Received:2010-10-09 Revised:2011-01-08 Published:2011-09-15 Online:2011-09-15

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摘要： 摘要 目的：研究不同剂量壬基酚(NP)对大鼠甲状腺功能的影响，探讨内分泌干扰物NP对大鼠甲状腺的毒性作用。方法：健康4周龄SD大鼠40只，随机分为溶剂对照组、低剂量组(40mg/kg)、中剂量组(80mg/kg)、高剂量组(200mg/kg)共四组，每组有大鼠10只，雌雄各半，灌胃染毒。染毒15天后观察其血清学、组织病理学改变。结果：1、染毒15天后，各实验组FT4、FT3 浓度呈逐渐降低的趋势，且与NP剂量呈负相关(FT4 r=-0.932；FT3 r=-0.926),与对照组相比差别有统计学意义(p<0.05)；中高剂量组与低剂量组相比差别有统计学意义(p<0.05)。2、各实验组的TSH浓度呈逐渐升高的趋势,且与NP剂量呈正相关(r=0.967),与对照组相比差别有统计学意义(p<0.05)；中高剂量组与低剂量组相比差别有统计学意义(p<0.05)。3、各实验组的SDH酶活性明显升高,与对照组相比差别有统计学意义(p<0.05)。4、各实验组均可见甲状腺组织增生，且增生的程度随NP染毒剂量增加而加重。结论：NP可造成甲状腺功能减低。

关键词: 壬基酚, 甲状腺, 功能

Abstract: Abstract Objective：To study the effects varying concentrations of nonylphenol on the structure and function of the thyroid gland in rats and mechanism.To provide the available information on endocrine disrupting chemical-NP to rats has on the toxic effect. Methods:40 healthy 4-weeks-old SD white rats were divided into 4 groups at random, group comprised of 5 male and 5 female rats. The treated rats were administered varying doses of NP(40mg/kg , 80mg/kg and 200mg/kg) by pouring stomach. The changes in serum hormone , histopathology and follicular cells ultramicroscopic structure were examined after 15 days. Results:1.Serum FT4 and FT3 levels decreased gradually as the dosage increased after 15 days and there is a negative correlation on doses of NP(FT4 r=-0.932; FT3 r=-0.926).The difference was statistical between random group and the treated groups(p<0.05).There was a significant difference between 40mg/kg and 80mg/kg ,200mg/kg on the doses of NP(p<0.05). 2.TSH levels increased. There is a positive correlation on doses of NP（r=0.967）.The difference was statistical between random group and the treated groups(p<0.05).There was a significant difference between 40mg/kg and 80mg/kg ,200mg/kg on the doses of NP(p<0.05). 3.SDH activity of the subject rats increased obviously , The difference was statistical between random group and the treated groups(p<0.05). 4.The hyperplastic thyroid structure can be observed on each group. The hyperplastic effect was in a dose dependent manner.

Key words: nonylphenol, thyroid gland, ultrastructure