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同型半胱氨酸对内皮细胞基质金属蛋白酶-1表达的影响

孟冬梅,毛用敏,赵莉莉,崔让庄,孙根义   

  1. 天津市胸科医院
  • 收稿日期:2011-12-20 修回日期:2012-06-13 出版日期:2012-10-15 发布日期:2012-10-15
  • 通讯作者: 孟冬梅

Effect of homocysteine on the expression of matrix metalloproteinase in human vascular endothelial cell

  • Received:2011-12-20 Revised:2012-06-13 Published:2012-10-15 Online:2012-10-15
  • Contact: Dong-Mei MENG

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摘要： 摘要 目的：观察同型半胱氨酸（homocysteine，Hcy）对血管内皮细胞(vein endothelia cell，VEC)基质金属蛋白酶-1(matrix metallopoeinase-1)基因表达的影响。方法：体外培养人脐静脉血管内皮细胞株CRL-1730(human umbilical vein vascular endothelia cell，HUVEC)，加入不同浓度的同型半胱氨酸刺激后收集细胞，提取细胞总RNA，逆转录—聚合酶链反应半定量检测基质金属蛋白酶-1（MMP-1）mRNA的表达。结果：病理浓度Hcy（≥50μmol/L）刺激HUVEC CRL-1730细胞后可诱导MMP-1 mRNA表达上调（P<0.01），同型半胱氨酸浓度与MMP-1 mRNA表达量呈正相关。结论：高浓度同型半胱氨酸使血管内皮细胞MMP-1的表达呈剂量依赖式上调，高浓度Hcy可能促进细胞外基质的降解，损害血管内皮的功能进而降低粥样硬化斑块的稳定性

关键词: 同型半胱氨酸, 血管内皮细胞, 基质金属蛋白酶-1, 基因表达, 细胞外基质

Abstract: Abstract Objective: To investigate the mechanism of artherosclerosis by disfunction of vascular endothelial cell with hyperhomocysteinaemia , we observe the effects of homocysteine on the genetic expression of matrix metalloproteinase-1 in human vascular endothelial cell. Method: Incubate HUVEC CRL-1730(human umbilical vein vascular endothelia cell) in vitro with difference concentration (0、10、50、100、1000μmol/L) of homocysteine, then collecte cells in 1,6 and 24 hours after incubation. To semiquantitative the mRNA expression of MMP-1by RT-PCR(reverse transcription- polymerase chain reaction) method. Result: Homocysteine of pathology concentration (50-1000μmol/L) increased the expression of MMP-1 significantly in a dose-depended manner(p<0.05). Conclusion: Pathology concentration homocysteine change the expression of MMP-1 in vascular endothelial cell and up-regulated the expression of MMP-1 a dose-depended manner. So homocysteine effects on the genetic expression of matrix metalloproteinase-1 in human vascular endothelial cell and then promoted extracellular matrix degradation,induced artherosclerosis and plaque disruptive .

Key words: homocysteine, vascular endothelial cell, matrix metalloproteinase, genetic expression, extracellular matrix