天津医药

• 实验研究 • 上一篇    下一篇

甲状腺素对甲状腺功能减低大鼠子代海马突触素表达的影响

孙亚婷1,汪蓓蕾2,杨丽娜1,张瑞3,郭刚3   

  1. 1. 天津医科大学代谢病医院内分泌研究所卫生部激素与发育重点实验室
    2. 天津医科大学代谢病医院
    3. 天津医科大学代谢病医院内分泌研究所
  • 收稿日期:2011-07-01 修回日期:2011-09-15 出版日期:2012-02-15 发布日期:2012-02-15
  • 通讯作者: 郭刚

Effects of Thyroid Hormone on the Expression of Synaptophysin in the Offspring Hippocampus of Hypothyroidism Rat Model

  • Received:2011-07-01 Revised:2011-09-15 Published:2012-02-15 Online:2012-02-15

PDF (PC)

2072

摘要： 目的: 探讨不同孕期补充不同剂量甲状腺素对甲状腺功能减低孕鼠子代海马突触素表达的影响。方法:选取1月龄Wistar大鼠160只，雌雄各半。将雌性大鼠随机分为正常组，甲低对照组，甲低孕鼠妊娠1~17 d（妊早期甲低）补充甲状腺素高、中、低剂量组，甲低孕鼠妊娠18~20 d（妊晚期甲低）补充甲状腺素高、中、低剂量组共8组，每组10只。各组均饲以低碘饲料，对照组饮用碘酸钾溶液，总碘摄入量相当于大鼠生理碘需要量，其余7组饮用去离子水。3个月后与正常雄性大鼠交配。高、中、低剂量组每100 g体质量每天分别补充甲状腺素3.5、2.0、0.5 μg。分别于新生当天、生后7d、生后14d、生后21d、生后28d，取8组子代大鼠的右侧大脑半球，应用免疫组织化学SABC法及图像分析技术检测海马突触素的表达。结果: 正常组不同时期的突触素表达水平均高于同时期甲低对照组（P＜0.01）。在新生当天，妊晚期甲低中剂量组与甲低对照组无显著差别（P>0.05）；妊早、晚期甲低低剂量组突触素表达水平均低于甲低对照组（P＜0.05）；妊早期甲低中、高剂量组，妊晚期甲低高剂量组突触素表达水平均高于甲低对照组（P＜0.01）。生后7d、生后14d、生后21 d、生后28d，妊早、晚期甲低低剂量组与甲低对照组无显著差别（P>0.05），其他各组的突触素表达水平均高于甲低对照组（P＜0.05）。结论: 孕鼠甲低导致子代大鼠海马突触素表达下降，甲低孕鼠子代大鼠海马突触素的表达受补充甲状腺素剂量及孕期的影响。

关键词: 甲状腺素, 甲状腺功能低下, 突触素, 大鼠, 免疫组织化学

Abstract: Objective：To explore the effects of thyroid hormone, given to the hypothyroidism pregnant rats, on the expression of synaptophysin in the offspring hippocampus. Methods：Eighty Wistar rats (female)were randomly divided into eight groups: normal group, hypothyroidism control group, hypothyroidism with pregnancy (1-17 d) + thyroid hormone (high, medium and low dosage) groups, and hypothyroidism with pregnancy (18-20 d) + thyroid hormone (high,medium and low dosage) groups. Rats were fed with low-iodine diet. Rats of normal group were given KIO3 solution, and rats of other groups were given deionized water. Rats were mated with normal male rats (n=80) after three months of feeding. According to 100 g of rat body weight, the concentrations of thyroid hormone for high, medium and low dosage were 3.5, 2.0, 0.5 μg/d.The hippocampus samples were taken from new-born, 7-day-old, 14-day-old,21-day-old and 28-day-old offspring rats in eight groups. Immunohistochemistry method and image analysis technique were used to detect the expression of synaptophysin in the hippocampus . Results：In different period rats , the expression level of synaptophysin was higher in normal group than that of hypothyroidism control group（P＜0.01）. In the newborn rats ,there was no significant difference in the expression level of synaptophysin between hypothyroidism with pregnancy (18-20 d) group supplied with medium dosage of thyroid hormone and hypothyroidism control group (P > 0.05), the expression level of synaptophysin was lower in hypothyroidism with pregnancy (1-17 d,18-20d) groups supplied with low dosage of thyroid hormone than that of hypothyroidism control group （P＜0.05）, the expression level of synaptophysin was higer in hypothyroidism with pregnancy (1-17 d) group supplied with thyroid hormone (me?dium ,high dosage)and hypothyroidism with pregnancy (18-20 d) group supplied with high dosage of thyroid hormone than that of hypothyroidism control group（P＜0.01）. In the 7-day-old,14-day-old，21-day-old and 28-day-old offspring rats, there was no significant difference in the expression level of synaptophysin between hypothyroidism with pregnancy (1-17 d,18-20d) groups supplied with low dosage of thyroid hormone and low-iodine control group (P > 0.05), the expression level of synaptophysin was higher in other groups than that of hypothyroidism control group（P＜0.05）.Conclusion：Hypothyroidism of pregnant rats results in the low expression of synaptophysin in off-spring hippocampus,the expression of synaptophysin in off-spring hippocampus is highly related to the supply dosage and supply time of thyroid hormone in hypothyroidism pregnant rats.

Key words: Thyroxine, Hypothyroidism, Synaptophysin, Rat, Imunohistochemistry