天津医药

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人脂肪间充质干细胞的致瘤性研究

秦秀军1,张伟2,李建国2,魏锦萍2,阚卫军3,李荣荣4   

  1. 1. 中国辐射防护研究院
    2. 太原，中国辐射防护研究院药物安全评价中心
    3. 武警山西总队医院
    4. 山东省红十字会介入医院
  • 收稿日期:2012-02-20 修回日期:2012-09-04 出版日期:2013-02-15 发布日期:2013-02-15
  • 通讯作者: 秦秀军

Tumorigenicity of Human Mesenchymal Stem Cells from Adipose Tissue

  1. 1. Drug Safety Evaluation Center in China Institute for Radiation Protection, Taiyuan 030006, China
    2.
  • Received:2012-02-20 Revised:2012-09-04 Published:2013-02-15 Online:2013-02-15
  • Contact: QIN Xiu jun

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摘要：

【摘要】目的 研究人脂肪间充质干细胞是否具有致瘤性，为其临床应用提供安全性依据。方法 96只裸鼠分为空白对照组、阳性对照组和脂肪间充质干细胞组，每组32只，雌雄各半。将传代第5代的人脂肪间充质干细胞接种至裸鼠皮下，分别于注射后3 d、1个月、3个月、6个月对动物进行解剖检查，观察其肿瘤形成情况；同时用软琼脂克隆形成实验观察人脂肪间充质干细胞形成集落的能力。结果 裸鼠体内致瘤实验中，脂肪间充质干细胞组和空白对照组动物大体解剖学观察和病理组织学检查均未见异常。软琼脂克隆形成实验显示人脂肪间充质干细胞未表现出在阻力介质中的克隆生长能力。结论 短期传代后的人脂肪间充质干细胞不具致瘤性，有一定安全性。

关键词: 间充质干细胞, 致瘤性, 裸鼠, 软琼脂克隆

Abstract:

[Abstract] Objective  To study the tumorigenicity of human mesenchymal stem cells from adipose tissue (hMSC-AT), and to provide safety data for its clinical application. Methods  Ninety-six nude mice were divided into blank control group， positive control group and adipose tissue-derived mesenchymal stem cell group (n=32 for each group). The hMSC-AT of the 5th passage was subcutaneously injected into nude mice. The mice were sacrificed 3 days, 1 month, 3 months and 6 months after injection respectively. The anatomical check was carried out to observe the tumor formation, and the ability to form colonies in hMSC-AT was observed by soft agar cloning method. Results  No abnormal cells were found by gross necropsy and histological examination in animals of hMSC-AT group and blank control group. In vitro test, hMSC-AT did not show the capacity for clonal growth in the medium. Conclusion  The hMSC-AT at low passage appears to be safe and is not tumorigenic.

Key words: Mesenchymal stem cells, Tumorigenicity, Nude mice, Soft agar cloning