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血必净对脓毒症大鼠巨噬细胞移动抑制因子和急性肾损伤的干预效果

王永明1,乔佑杰2,李家瑞3,尚跃丰4   

  1. 1. 天津市天津医院
    2. 天津市人民医院
    3. 天津市天津医院急诊医学科
    4. 天津市天和医院心内科
  • 收稿日期:2014-03-11 修回日期:2014-06-17 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 乔佑杰

Effect of Xuebijing Injection on MIF Expression and Acute Kidney Injury in Rats with Sepsis

  • Received:2014-03-11 Revised:2014-06-17 Published:2014-10-15 Online:2014-10-15
  • Contact: QIAO You jie

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摘要：

【摘要】目的 观察脓毒症急性肾损伤大鼠肾组织及血清巨噬细胞移动抑制因子（MIF）的表达规律，并探讨血必净注射液对脓毒症大鼠MIF 表达的影响及急性肾损伤的防治作用。方法 采用盲肠结扎穿孔术（CLP）制备脓毒症模型。健康SD 大鼠80 只按随机数字表法分为假手术组（n=16）、模型组(n=32)和血必净组（n=32），分别于术后2、 8、24 和48 h 4 个时间点处死取材。采用实时定量PCR 检测肾组织MIF mRNA 表达, 酶联免疫法检测血MIF 表达；同时用生化分析仪测定血清肌酐（Cr）水平。 结果 与假手术组比较，模型组大鼠肾组织MIF mRNA 表达于CLP 后 8、24、48 h 显著升高（P < 0.05），血清MIF 及Cr 水平于CLP 后24、48 h 显著增高（P < 0.05）；与模型组比较，血必净组大鼠肾组织MIF mRNA 于2、8、24、48 h 明显下降（P < 0.05），血清MIF 及Cr 水平于24、48 h 亦显著下降（P < 0.05）。结论 MIF 可能作为晚期炎症因子参与脓毒症大鼠急性肾损伤的病生理过程。血必净注射液可抑制 MIF 的表达，对脓毒症大鼠肾损伤具有保护作用。

关键词: 脓毒症, 巨噬细胞移动抑制因子, 急性肾损伤, 血必净注射液

Abstract:

[Abstract] Objective  To investigate the expression of macrophage migration inhibitory factor (MIF) in serum and renal tissue of septic rats with actue kidney injury (AKI), and to explore the effect of Chinese traditional medicine-Xuebijing injection on MIF expression as well as on acute kidney injury in rats with sepsis. Methods  Sepsis model was reproduced in rats with cecal ligation and puncture (CLP)．Eighty healthy SD rats were randomly divided into three groups：sham operation group（n=16）, CLP model group (n=32), and xuebijing group(n=32)．All rats were sacrificed at either 2, or 8, or 24 and or 48 hours after operations．MIF mRNA levels in renal tissues of septic rats were semi-quantified by Real-time PCR．The content of MIF in serum was determined by enzyme linked immunosorbent assay (ELISA). Serum creatinine (Cr) contents were measured by automatic biochemistry analyze. Results  Compared with sham operation group, transcription of MIF mRNA in renal tissues of model group were significantly enhanced at 8, 24 and 48 hours after operations (P < 0.01). Both contents of MIF and creatinine level in serum of model group rose obviously at 24 and 48 hours after operation (P < 0.01)； Compared with model group, the transcription of MIF mRNA in renal tissues of xuebijing group decrease obviously at 2, 8, 24 and 48 hours (P < 0.01) and both contents of MIF and creatinine in serum of xuebijing group drop remarkably at 24 and 48 hours (P < 0.01). Conclusion  MIF is a kind of late cytokine which might participate in the pathogenesis of AKI in rats with sepsis．Xuebijing injection can inhibit MIF expression, and possess the protective effects on the kidney in rats with sep? sis.

Key words: Sepsis, macrophage migration inhibition factor, acute kidney injury (AKI), Xuebijing injection