天津医药

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神经纤毛蛋白1、2在大肠癌组织中的表达及意义

徐卫国1,苗娜1,孙红2,刘云3,张世伟3,郝小惠4   

  1. 1. 河北联合大学附属医院肿瘤外科
    2. 河北联合大学基础医学院
    3. 河北联合大学附属医院
    4. 河北联合大学
  • 收稿日期:2013-12-16 修回日期:2014-06-09 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 苗娜

Expression and Clinical Significance of Neuropilin-1 and 2 in Colorectal Carcinoma

  • Received:2013-12-16 Revised:2014-06-09 Published:2014-10-15 Online:2014-10-15
  • Contact: MIAO Na

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摘要：

【摘要】目的 探讨神经纤毛蛋白（NRP）-1、2 在大肠癌组织中的表达及其与微血管密度（MVD）的相关性，以揭示NRP-1、2 与肿瘤血管生成的关系。方法 采用免疫组织化学方法检测66 例大肠癌患者的癌组织、癌旁组织及正常组织中NRP-1、2 的表达情况，以CD105 标记肿瘤新生血管内皮细胞，计数MVD 值。结果 （1）NRP-1、NRP-2 在大肠癌组织、癌旁组织、正常组织中的表达阳性率分别为71.2%、25.8%、0，及80.3%、15.2%、0，各组间比较差异有统计学意义(P＜0.01)。（2）MVD 在大肠癌组织、癌旁组织及正常组织中的计数分别为35.682±5.542、14.485±3.301、 6.864±1.771，各组相比差异有统计学意义（F=986.654，P＜0.01）。（3）不同年龄、性别、肿瘤的部位及分化程度的患者 NRP-1、2 的表达差异无统计学意义。肿瘤＞5 cm 的患者NRP-1 阳性表达率较高，肿瘤侵及浆膜及以外、有淋巴结转移的患者NRP-1、2 的阳性表达率均较高，不同的Dukes 分期患者NRP-1、2 阳性表达差异亦有统计学意义（P＜ 0.05）。（4）大肠癌患者不同性别、年龄及肿瘤大小、部位和组织分化程度MVD 的表达差异无统计学意义，而浸润到浆膜及以外、有淋巴结转移、Dukes 分期高的患者相关MVD 的表达增加(P＜0.01)。（5）大肠癌组织中NRP-1（r=0.599， P＜0.01）、NRP-2（r=0.484，P＜0.01）的表达与MVD 计数均呈正相关关系。结论 NRP-1、2 在大肠癌的血管新生中起重要作用，并且与大肠癌侵袭和转移关系密切。

关键词: 大肠癌, 神经纤毛蛋白, 微血管密度, 免疫组化, 结直肠肿瘤, 血管内皮细胞, 肿瘤血管生成

Abstract:

[Abstract] Objective  To observe the expression of Neuropilin (NRP)-1, 2, to calculate micro vessel density (MVD) in colorectal cancer, paraneoplastic tissues, normal tissues, and to investigate the correlation between NRP-1, 2 and MVD to understand the role of NRP-1, 2 in the process of tumor angiogenesis. Methods  Expression of NRP-1 and NRP-2 were studied by immunohistochemistry in 66 specimens from colorectal cancer, paraneoplastic tissues and normal tissues. MVD was assessed based on CD105 immunohistochical staining. Results  (1) The positive expression of NRP-1, 2 in colorectal cancer, paraneoplastic tissues, normal tissues were 71.2%, 25.8%, 0; 80.3%, 15.2%, 0 respectively. There was a statistical difference between them (P＜0.05). (2)The quantity of MVD in colorectal cancer, paraneoplastic tissues and normal tissues were 35.682±5.542, 14.485±3.301 and 6.864±1.771 respectively. There was a statistical difference between them (P＜0.05). (3) Expression of NRP-1, 2 had a good correlation with the tumor size, infiltrating depth, lymph node metastasis, Dukes stag? ing (P＜0.05), but no correlation was found with the tumor position, histological types, differentiation degree and the gender or age of patients (P＞0.05). (4) The MVD value had no correlation with the gender or age of patients, tumor size, position, histological types (P＞0.05), but had a good correlation with infiltrating depth, lymphatic metastasis and Dukes staging (P＜ 0.05). (5) There was a positive correlation between NRP-1, 2 and MVD value  (P＜0.01). Conclusion  NRP-1, 2 may play an important role in the angiogenesis of the colorectal cancer, and it correlates with the invasion and metastasis of colorectal cancer closely.

Key words: colorectal carcinoma, neuropilins, Microvessel density, Immunohistochemistry, colorectal neoplasms, vascular endothelial cell, tumor angiogenesis