天津医药

天津医药

• 细胞与分子生物学 • 上一篇    下一篇

SDF-1/CXCR4对大肠癌细胞株SW480增殖、迁移及侵袭的影响及意义

袁丽倩1,郑淑芳2   

  1. 1. 辽宁医学院武警后勤学院附属医院研究生培养基地
    2. 辽宁医学院武警后勤学院附属医院病理科
  • 收稿日期:2014-06-30 修回日期:2014-09-18 出版日期:2014-11-15 发布日期:2014-11-15
  • 通讯作者: 袁丽倩

Effects and Significance of SDF-1/CXCR4in Proliferation, Migration and Invasion of Colorectal Cancer Cell Line SW480

YUAN Li qian1,ZHENG Shu fang2   

  1. 1. Postgraduate Training Basement,Affiliated Hospital of Logistics College of CAPF Liaoning Medical College
    2. Department of Pathology, Affiliated Hospital of Logistics College of CAPF, LiaoningMedical College
  • Received:2014-06-30 Revised:2014-09-18 Published:2014-11-15 Online:2014-11-15
  • Contact: YUAN Li qian

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摘要：

【摘要】  目的  探讨基质细胞衍生因子-1(SDF-1)及其特异性受体CXC趋化因子受体4（CXCR4）对大肠癌细胞SW480增殖、迁移及侵袭能力的影响及意义。  方法  取对数生长期大肠癌细胞SW480分为对照组(未经任何处理)、SDF-1组（加入100μg/L SDF-1）、SDF-1＋AMD3100混合组(向细胞中加入1mg/L AMD3100，孵育2h后加入100μg/LSDF-1)、AMD3100组（加入1mg/L AMD3100）。免疫组化法检测SW480细胞中CXCR4蛋白表达情况；RT-PCR法检测SW480细胞中CXCR4mRNA的表达情况，以及外源性SDF-1和AMD3100作用后CXCR4mRNA表达水平的变化；MTT增殖实验、Transwell迁移及侵袭实验分别检测SDF-1以及AMD3100对SW480细胞增殖、迁移及侵袭能力的影响。  结果  SW480细胞中CXCR4蛋白呈阳性表达（阳性率80%）。SW480细胞中有CXCR4mRNA的表达，100μg/LSDF-1促使CXCR4mRNA表达水平进一步上调，且能被1mg/L AMD3100阻断。SDF-1组细胞增殖活性(0.847±0.039）高于对照组(0.624±0.011)和SDF-1＋AMD3100混合组(0.607±0.016)，AMD3100组(0.456±0.032)低于对照组和SDF-1＋AMD3100混合组(F=108.030，P＜0.05)。Transwell小室迁移及侵袭实验中SDF-1组穿膜细胞数(个：98.7±5.8、33.7±6.2)均多于对照组(21.0±2.2、6.1±2.3)、SDF-1＋AMD3100混合组(18.5±8.4、8.5±2.8)和AMD3100组(12.1±3.2、2.1±1.0)，后3组间比较差异无统计学意义。  结论  SDF-1/CXCR4生物轴可促进大肠癌细胞SW480的增殖、迁移及侵袭。

关键词: 肠肿瘤, 大肠, 趋化因子CXCL12, 受体, CXCR4, 细胞增殖, 细胞运动, 细胞迁移, 细胞侵袭, AMD3100

Abstract:

[Abstract]   Objective   To discuss the influence and significance of stromal cell-derived factor1(SDF -1) and its
specific receptor CXC chemokine receptor4(CXCR4) in proliferation, migration and invasion ability of SW480colorectal cancer cells.  Methods   The colorectal cancer cell line SW480in logarithmic phase was divided into four groups: control group (with no any processing), SDF-1group (added100μg/L SDF-1), SDF-1＋1mg/L AMD3100mixed group (added1mg/L AMD3100for 2hours, then added100μg/L SDF-1) and AMD3100group (added1mg/L AMD3100). Immunohisto?chemistry method was used to detect the protein expression of CXCR4in SW480cells. The expression of CXCR4mRNA in SW480cells was detected by RT-PCR before and after SDF-1and AMD3100treatment. MTT assay and transwell chamber were used to test the changes of proliferation, migration and invasion ability of SW480cells before and after SDF-1and AMD3100treatment.   Results   The result of immunohistochemistry showed that CXCR4protein was expressed in SW480 cells (positive rate=80%). CXCR4mRNA was expressed in SW480cells. The expression of CXCR4mRNA was up-regulat?ed by SDF-1(100μg/L), which could be inhibited by AMD3100(1mg/L). The proliferation activity was higher in SDF-1group (0.847±0.039) compared to that in control group (0.624±0.011) and SDF-1＋AMD3100mixed group(0.607±0.016).The proliferation activity was lower in AMD3100group (0.456±0.031) than that in control group and SDF-1＋AMD3100 mixed group (F=108.03, P＜0.05). The number of transmembrane cells was more in SDF-1group (98.7±5.8, 33.7±6.2) than that in control group (21.0±2.2, 6.1±2.3), SDF-1＋1mg/L AMD3100mixed group (18.5±8.4, 8.5±2.8) and AMD3100group (12.1±3.2, 2.1±1.0) detected by transwell chamber experiment. However, there were no statistical differences between three groups.   Conclusion   The biological axis SDF-1/CXCR4 can promote the proliferation, migration and invasion in colorectal cancer cell line SW480.

Key words: intestinal neoplasms, intestine large, chemokine CXCL12, receptors, CXCR4, cell proliferation, migration movement, cell migration, Cell invasion, AMD3100