天津医药

• 细胞与分子生物学 • 上一篇    下一篇

γH2AX对肺癌放射敏感性的预测效果*

韩宝林   

  1. 天津市宝坻区人民医院
  • 收稿日期:2014-01-14 修回日期:2014-04-15 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 韩宝林

Assessing Radiosensitivity of Lung Cancer with the Expression of γH2AX

  • Received:2014-01-14 Revised:2014-04-15 Published:2014-09-15 Online:2014-09-15

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摘要：

【摘要】目的 探讨人肺癌细胞受照后γH2AX表达量变化与肺癌细胞放射敏感性之间的关系。方法 选择肺腺癌A549和小细胞肺癌SBC-3细胞株，采用细胞克隆形成实验检测经不同剂量（0、2、4、6、8、10Gy）照射后A549 和SBC-3细胞的克隆形成率，并绘制细胞生存曲线；应用Western blot检测经2Gy放射剂量照射后不同时间点（0 min、30min、1h、2h、6h、12h、24h、36h、48h）的细胞中γH2AX蛋白表达量。分析γH2AX蛋白表达量变化与肿瘤细胞放射敏感性的相关性。结果 小细胞肺癌SBC-3细胞的放射敏感性明显高于肺腺癌A549细胞。两种细胞受照后1h和6h，γH2AX的表达量均与肿瘤细胞的平均致死剂量（D0）相关。结论 γH2AX可以作为肺癌细胞放射敏感性的一个预测指标。

关键词: γH2AX蛋白, 肺癌, 放射敏感性

Abstract:

[Abstract] Objective To observe the relationship between expression changes ofγH2AX and the radiosensitivity of lung cancer cells in vitro.Methods The radiosensitivity of lung cancer cell lines A549and SBC-3was measured by clone forming assay. The DSBs damage of lung cancer cell lines A549and SBC-3was determined by Western blot assay.Re? sults The clone forming rates of lung cancer cell lines A549and SBC-3were gradually decreased with the increased radia? tion dose. γH2AX expression was related to the cell radiosensitivity1hour and6hours after radiated. Conclusion The phosphorylated histoneγH2AX is a powerful tool to monitor DNA DSBs and to predict the radiosensitivity in lung cancer ra? diotherapy.

Key words: γH2AX protein, lung cancer, radiosensitivity