天津医药

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人参皂苷Rg1对脑缺血再灌注大鼠nNOS，iNOS表达的影响

屈惠莹,袁静   

  1. 辽宁医学院
  • 收稿日期:2014-05-23 修回日期:2014-07-03 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 屈惠莹

Effects of Ginsenoside Rg1on nNOS and iNOS Expressions in Rat Brain Tissue after Cerebral Ischemia Reperfusion

  • Received:2014-05-23 Revised:2014-07-03 Published:2014-09-15 Online:2014-09-15
  • Contact: QU Huiying

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摘要： 【摘要】 目的 探讨人参皂苷Rg1对脑缺血再灌注大鼠脑组织一氧化氮合酶（NOS）表达的影响。方法 将大鼠随机分成假手术组，模型组（脑缺血再灌注损伤组），人参皂苷Rg1 10，20，40mg/kg组，尼莫地平组（阳性药物对照组），每组10只。采用大鼠中动脉栓塞法制作大鼠脑缺血再灌注模型，观察大鼠再灌注后神经功能缺损情况，采用比色法检测大鼠nNOS，iNOS和一氧化氮（NO）的含量，应用免疫组化和免疫印迹法检测脑组织缺血再灌注后nNOS，iNOS的表达。结果 （1）人参皂苷Rg1各组大鼠脑缺血后神经功能评分明显低于模型组（p<0.05）；（2）与模型组相比，人参皂苷Rg1各组随浓度增高nNOS含量及表达增高（p<0.05），iNOS含量及表达明显降低（p<0.05）；（3）免疫组化和免疫印迹的结果也证明这一趋势。结论 人参皂苷Rg1防治大鼠脑缺血再灌注损伤的机制可能与激活nNOS，抑制iNOS有关，且以高剂量效果较好。

关键词: 人参皂苷Rg1, 脑缺血再灌注, 一氧化氮合酶

Abstract:

[Abstract] Objective To investigate the effects of Ginsenoside Rg1on the expression of nitric oxide synthase (NOS) in brain tissue after cerebral arterial thrombosis in adult rats. Methods Thirty rats were randomly divided into the sham-operative group, cerebral ischemia-reperfusion group, Ginsenoside Rg1-L group, Ginsenoside Rg1-M group, Ginsen? oside Rg1-H group and nimodipine group (n=5for each group). The ischemia-reperfusion rat model was established by mid? dle cerebral artery occlusion. The neurological score after reperfusion was observed. The levels of nitric oxide (NO), neuronal NOS (nNOS) and inducible NOS (iNOS) were detected by nitrate reduction method and colorimetric method. The expressions of nNOS and iNOS after reperfusion were analyzed by immunohistochemistry and Western blot assay.Results (1) The neu? rological scores after cerebral ischemia were significantly lower in Rg1-L group, Rg1-M group and Rg1-H group than those of cerebral ischemia-reperfusion group（2.40±0.55,1.80±0.84, 1.60±0.89vs3.20±0.84，P<0.05). (2) Compared with those of model group, serum levels of NO and iNOS were reduced, and nNOS levels increased, in three groups of Rg1. (3) Compared with those of model group, the expression of nNOS was significantly increased，and iNOS expression was significantly re? duced, in three groups of Rg1. Conclusion The preventive effects of Ginsenosides Rg1on cerebral ischemia-reperfusion injury may be associated with the activation of nNOS and the inhibition of iNOS.

Key words: Ginsenoside Rg1, Cerebral Ischemia Reperfusion, Nitric oxide synthase