天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1093-1096.doi: 10.11958/j.issn.0253-9896.2015.10.002

• 细胞与分子生物学 • 上一篇    下一篇

黄芪提取物对大鼠骨髓源性内皮祖细胞的作用

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  1. 南阳理工学院医学实验中心
  • 收稿日期:2015-04-16 修回日期:2015-07-22 出版日期:2015-10-15 发布日期:2015-10-22

Effect of astragalus extracts on bone marrow-derived endothelial progenitor cells in rats

  1. Medical Experimental Center, Nanyang Institute of Technology, Nanyang 473004, China
  • Received:2015-04-16 Revised:2015-07-22 Published:2015-10-15 Online:2015-10-22

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摘要：

摘要： 目的 探讨黄芪提取物对大鼠骨髓源性内皮祖细胞 （EPCs） 黏附、 迁移、 活力、 血管形成能力及内皮型一氧
化氮合成酶 （eNOS） 表达的影响。方法 体外培养、 分离和鉴定 EPCs， 设 10-4、 10-3、 10-2 g/L 黄芪提取物组和对照组。
倒置显微镜下观察并比较各组 EPCs 黏附、 迁移、 血管形成能力的差异； MTT 法检测 EPCs 的活力变化； RT-PCR 法检
测 EPCs 中 eNOS mRNA 的表达；Western blot 检测 EPCs 中 eNOS 蛋白的表达。结果 与对照组相比， 不同浓度黄芪
提取物组促 EPCs 黏附、 迁移、 血管形成能力均显著增强， 且呈浓度依赖性(F 值分别为 15.256、 13.633、 97.549， 均 P <
0.05)； EPCs 的活力显著增加， 呈时间 （F 时间=9.755） 和浓度依赖性 （F 组间=10.018）； 且 EPCs 中 eNOS mRNA 和蛋白的表
达水平均明显升高， 呈浓度依赖性(F 值分别为 56.356、 77.125， 均 P < 0.05)。结论 黄芪提取物具有调控 EPCs 促血
管新生的作用， 该作用可能与上调 eNOS 的表达水平密切相关。

关键词: 黄芪, 骨髓祖代细胞, 细胞黏附, 细胞运动, 内皮祖细胞, 血管新生, 内皮型一氧化氮合成酶

Abstract:

Abstract: Objective To explore the effect of astragalus extracts on adherence, migration, cell viability, microvascular
formation, and eNOS expression in bone marrow-derived endothelial progenitor cells (EPCs) of rats. Methods EPCs were
cultured, isolated and identified in vitro and divided into four groups: low titer group (10-4 g/L of astragalus extracts), middle
titer group (10-3 g/L of astragalus extracts), high titer group (10-2 g/L of astragalus extracts) and control group. The effects of
astragalus extract on adhesion, migration or microvascular formation were observed under the inverted microscope and com⁃
pared between all groups; Cell viability was detected by MTT assay; mRNA transcription and protein expression levels of en⁃
dothelial nitric oxide synthase (eNOS) in EPCs were detected by reverse transcription PCR (RT-PCR) and Western blot re⁃
spectively. Results Compared with the control group, cell adhesion, migration and microvascular formation of EPCs all en⁃
hanced with addition of astragalus extracts in a dose dependent manner (F=15.256, 13.633, 97.549 respectively, and P <
0.05 in all cases); Cell vitality of EPCs increased with administration of astragalus extracts in a time and dose dependant
manner. (F=9.755 for time and F=10.18 for dose). mRNA transcription and protein expression of eNOS in EPCs were up-reg⁃
ulated with addition of astragalus extracts in a dose dependent manner (F=56.356 and 77.125 respectively, and P < 0.05 in
both cases). Conclusion Astragalus extracts play important role in angiogenesis in EPCs probably through up-regulating
expressions of eNOS.

Key words: Astragalus membranaceus, myeloid progenitor cells, cell adhesion, cell movement, endothelial progenitor cells, angiogenesis, endothelial nitric oxide synthase