天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1097-1099.doi: 10.11958/j.issn.0253-9896.2015.10.003

• 细胞与分子生物学 • 上一篇    下一篇

上调miR-34c-5p对鼻咽癌细胞HONE-1增殖和侵袭的影响

  

  1. 1广州医科大学附属第二医院 VIP产科 （邮编 510260）， 3胃肠外科， 5病理科， 6检验科； 2广东省妇幼保健院； 4佛山市第一人民医院
  • 收稿日期:2015-01-23 修回日期:2015-05-22 出版日期:2015-10-15 发布日期:2015-10-22

The effect of up-regulating miR-34c-5p on the proliferation and invasion of nasopharyngeal#br# carcinoma cell line HONE-1

  1. 1-3, 5-6 The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China； 2 Guangdong Provincial
    Maternity and Child Care Center; 4 The First People′s Hospital of Foushan
  • Received:2015-01-23 Revised:2015-05-22 Published:2015-10-15 Online:2015-10-22

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摘要： 摘要： 目的 探讨上调 miR-34c-5p 对鼻咽癌细胞 HONE-1 增殖和转移能力的影响。方法 利用定量 PCR 分
析 miR-34c-5p 在鼻咽癌细胞株 HONE-1 和正常鼻咽细胞株 NP69 中的表达情况； 利用克隆形成实验和 Transwell
实验分析上调 miR-34c-5p 表达对 miR-34c-5p mimic 转染组、 miR-34c-5p 对照序列组和未处理组增殖和侵袭能力
的影响。结果 miR-34c-5p 在鼻咽癌细胞株 HONE-1 的表达水平低于正常鼻咽细胞株 NP69； miR-34c-5p mimic
转染组鼻咽癌细胞 HONE-1 的克隆形成和侵袭能力明显低于 miR-34c-5p 对照序列组和未处理组（均 P＜0.05）。
结论 miR-34c-5p 的下调与鼻咽癌的生物学功能增殖和侵袭能力相关， 是鼻咽癌治疗的潜在靶点。

关键词: 鼻咽肿瘤, 细胞增殖, 肿瘤侵润, 体外研究, miR-34c-5p, HONE-1

Abstract: Abstract：Objective To investigate the effect of up-regulating miR-34c-5p on cloning formation and migration of
nasopharyngeal carcinoma cell line HONE-1 in vitro. Methods Expression levels of miR-34c-5p in nasopharyngeal cell
line NP69 and nasopharyngeal carcinoma cell line HONE-1 were investigated by real time quantitative PCR; Cloning Forma⁃
tion Test and Transwell Migration Assay were used to explore the effect of up-regulating miR-34c-5p on proliferation and
migration of nasopharyngeal carcinoma cell line HONE-1. Results The expression of miR-34c-5p in nasopharyngeal car⁃
cinoma cell line HONE-1 was lower than that in normal nasopharyngeal cell line NP69. Up-regulating miR-34c-5p signifi⁃
cantly suppressed the cloning formation and migration capacity, compared to those of NP69 cell line and HONE-1 with nor⁃
mal level of miR-34c-5p（P＜0.05） . Conclusion Down-regulating miR-34c-5p involve in proliferation and migration of
nasopharyngeal carcinoma and may be a used as a new therapeutic target for nasopharyngeal carcinoma.

Key words: nasopharyngeal neoplasms, cell proliferation, neoplasm invasiveness, in vitro , miR-34c-5p, HONE-1