天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (10): 1125-1127.doi: 10.11958/j.issn.0253-9896.2015.10.011

• 实验研究 • 上一篇    下一篇

大鼠肾发育中Wnt4蛋白的表达

  

  1. 1辽宁医学院组织胚胎学教研室 （邮编 121001）； 2辽宁医学院图书馆
  • 收稿日期:2015-03-09 修回日期:2015-07-10 出版日期:2015-10-15 发布日期:2015-10-22

The expression profile of Wnt4 in rat kidney during renal development

  1. 1 Department of Histology and Embryology of Liaoning Medical University, Liaoning 121001, China; 2 Library of
    Liaoning Medical University
  • Received:2015-03-09 Revised:2015-07-10 Published:2015-10-15 Online:2015-10-22

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摘要：

摘要：目的 探讨大鼠肾发育中 Wnt4 蛋白的表达规律及 Wnt4 蛋白对大鼠肾发育的影响。方法 选取胚龄
（E） 18、 20 d 和生后（P） 0、 1、 3、 5 和 7 d 的大鼠肾脏， 应用免疫组织化学技术， 定性分析该时间段内大鼠肾组织中
Wnt4 蛋白的表达部位及其变化情况； 应用蛋白印迹检法， 定量分析该时间段内大鼠肾组织中 Wnt4 蛋白表达量的变
化。结果 免疫组化结果显示， 从 E 18 d～P 7 d， Wnt4 蛋白在近端小管， 在生肾区的输尿管芽、 逗号小体以及 S 小体
的表达较强， 在远端小管的表达较弱， 在肾小体的表达由强到弱。蛋白印迹检测结果显示， Wnt4 蛋白的表达量 E 18
d 时开始逐渐降低， P 1 d 时到达低点， 然后开始回升， P 7 d 时再次降低。结论 在大鼠肾发育中， Wnt4 蛋白通过启
动经典的 Wnt/β-catenin 信号通路参与调控肾单位的发育， 启动非经典的 Wnt/PCP 信号通路参与调控近端小管的延
长， 其可能与肾单位的形成以及近端小管的发育密切相关。

关键词: 肾小管, 近端, Wnt 蛋白质类, 大鼠, Sprague-Dawley , 动物实验, Wnt 信号通路, 肾发育

Abstract:

Abstract: Objective To explore the expression profile of Wnt4 in rat kidney during renal development and its effect on
renal development. Methods Rats with embryonic age of 18 days (E 18 d) , 20 days (E 20 d) as well as postnatal age of 0 day
(P 0 d), 1 day (P 1 d), 3 days (P 3 d), 5 days (P 5 d) and 7 days (P 7 d) were selected. Expression levels of Wnt4 in rat kidney
during renal development were quantified by immunohistochemistry and Western blot in all time points. Results Immuno⁃
histochemistry analysis showed that during E 18 d to P 7 d, Wnt4 mainly expressed in proximal tubules, ureteric bud, comma
shaped bodies and S shaped bodies of nephrogenic zone; the expression in the distal tubule was weak; the expression in renal
corpuscle decreased with time; Western blot analysis showed that the expression of Wnt4 in rat kidney began to decrease from
E 18 d and reached bottom at P 1 d then rise again until P 7 d when it dropped again. Conclusion During renal development,
Wnt4 proteins were involved in the development of the nephrogenic zone through regulating canonical Wnt/β-catenin signaling
pathway, and was involved in extension of proximal tubules by inducing the non canonical Wnt/PCP signaling pathway. Expression
of Wnt4 protein in rat kidney was closely related to nephron formation and development of proximal tubules.

Key words: kidney tubules, proximal, Wnt proteins, rats, Sprague- Dawley, animal experimentation, Wnt signaling pathway, kidney development