天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (12): 1394-1397.doi: 10.11958/j.issn.0253-9896.2015.12.013

• 实验研究 • 上一篇    下一篇

CEUS 定量分析L-NAME 致兔高血压性肾损伤的实验研究

孟祥鹿1,2，张国慧3，王文红2△，邵红2，郭君3，赵效新1,2，顾程1,2，张晓丹4   

  1. 1天津医科大学研究生院（邮编300070）；2天津市人民医院；3北京，航天中心医院超声科；4河北大学附属医院超声科
  • 收稿日期:2015-06-03 修回日期:2015-08-18 出版日期:2015-12-15 发布日期:2015-12-11
  • 通讯作者: △通讯作者E-mail:wangwenhong2014@126.com E-mail:592216657@qq.com
  • 作者简介:孟祥鹿（1989），女，硕士在读，主要从事腹部疾病的研究
  • 基金资助:
    天津市卫生局基金资助项目（2011KZ52）

Quantitative analysis of renal injury using CEUS in hypertensive rabbits model induced by L-NAME

MENG Xianglu1,2, ZHANG Guohui3, WANG Wenhong2△, SHAO Hong2, GUO Jun3, ZHAO Xiaoxin1,2, GU Cheng1,2, ZHANG Xiaodan4   

  1. 1 Tianjin Medical University Graduate School, Tianjin 300070, China; 2 Tianjin Union Medicine Centre; 3 Ultrasound Department, Aero Space Center Hospital; 4 Ultrasound Department, Affiliated Hospital of Hebei Medical University
  • Received:2015-06-03 Revised:2015-08-18 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail：wangwenhong2014@126.com E-mail:592216657@qq.com

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摘要： 目的应用超声造影（CEUS）定量评价一氧化氮合成酶抑制剂N-硝基-L-精氨酸甲酯（L-NAME）致兔高血压肾损伤后肾皮质的血流灌注变化。方法建立L-NAME 致兔高血压肾损伤模型，分别于给药前及给药后第2、 4、6、8 周行CEUS 检查及血清胱抑素C（CysC）等生化检查，将所获得的时间-强度曲线（TIC）曲线下面积（AUC）等相关参数进行定量分析，并将AUC 与血清CysC 进行相关性分析。结果兔血清CysC 在给药6 周后明显升高，明显早于血肌酐（Scr）及血尿素氮（BUN）。给药后AUC 先减小后增大，开始增强时间（RT）延长，峰值强度（PI）减低，平均通过时间（MTT）、峰值减半时间（HPT）及达峰时间（TTP）延长。CEUS 所得AUC 与血清CysC 呈显著正相关（r= 0.950，P＜0.001)。结论L-NAME 致兔高血压肾损伤模型操作简单、可重复性好，可用于对高血压肾损伤临床前期及临床期的药物实验研究。CEUS 结合血清CysC 可作为临床评价高血压肾损伤患者肾功能异常的良好检查方法。

关键词: 高血压, NG-硝基精氨酸甲酯, 兔, 高血压肾损伤, 超声造影, 血清胱抑素C, 肾血流灌注

Abstract: Objective To assess the value of contrast-enhanced ultrasound (CEUS) on quantitative analysis of re⁃ nal cortex perfusion in hypertensive rabbits model. Methods Hypertensive rabbit modal (n=10) were established by inject⁃ ing N-nitro-L-arginin methylester (L-NAME). CEUS and Cystatin C (CysC) serum level analysis were performed at differ⁃ ent time points: before and the 2nd, 4th, 6th and 8th week after injecting L-NAME. Time-intensity curve and area under curve (AUC) were analyzed quantatively while correlation of AUC and CysC were also analyzed. Results Serum level of Cys C in⁃ creased significantly at the 6th week after L-NAME administration which is earlier than the increase of serum levels of Scr and BUN. AUC decreased at first then increased after L-NAME administration. Upon addition of L-NAME, rise time (RT) and peak intensity (PI) decreased while mean transit time (MTT), time from peak to one half (HPT) and time to peak (TTP) in⁃ creased. Our study confirmed a positive correlation between AUC and Cys C (r=0.950, P < 0.001). Conclusion Setting up rabbits model by L-NAME is convenient and reproducible, which is an useful tool in experimental study of preclinical and clinical phase of hypertensive renal injury. CEUS combining with CysC serum level analysis is considered as an effective technology for evaluating renal function in hypertensive patients.

Key words: hypertension, NG-nitroarginine methylester, rabbits, hypertensive renal injury, contrast-enhanced sonography, cystatinC, renal perfusion