天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (12): 1413-1416.doi: 10.11958/j.issn.0253-9896.2015.12.018

• 临床研究 • 上一篇    下一篇

OCT4 和P27kip1 蛋白在肾透明细胞癌中的表达及其相关性

关有良1, 臧东钰2,王丹3△   

  1. 1辽宁医学院附属第一医院泌尿外科（邮编121001）；2辽宁医学院附属第三医院胸外科；3辽宁医学院组织胚胎学教研室
  • 收稿日期:2015-03-16 修回日期:2015-06-11 出版日期:2015-12-15 发布日期:2015-12-11
  • 通讯作者: △通讯作者E-mail：595680903@qq.com E-mail:wanganan1997@sina.com
  • 作者简介:关有良（1973），男，副主任医师，硕士，主要从事肾癌发病机制研究
  • 基金资助:
    辽宁省自然科学基金资助项目（2013022009）；辽宁医学院校长基金项目（XZJJ20130243）

The expression of OCT4 and P27kip1 in renal hyaline cell carcinoma and their correlations

GUAN Youliang1, ZANG Dongyu2, WANG Dan3△   

  1. 1 Department of Urinary Surgery of the First Affiliated Hospital, Liaoning Medical College, Jinzhou 121001,China; 2 Department of Thoraxes Surgery of the Third Affiliated Hospital, Liaoning Medical College; 3 Department of Histology and Embryology, Liaoning Medical College
  • Received:2015-03-16 Revised:2015-06-11 Published:2015-12-15 Online:2015-12-11
  • Contact: △Corresponding Author E-mail：595680903@qq.com E-mail:wanganan1997@sina.com

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摘要： 目的研究OCT4 和P27kip1 蛋白在肾透明细胞癌中的表达及其相关性。方法采用免疫组织化学方法对 24 例肾透明细胞癌标本OCT4 和P27kip1 的表达进行检测。观察OCT4 和P27kip1 在肾癌组织中的阳性表达百分比及细胞分布情况。考察OCT4 和P27kip1 在性别、年龄（＜60 岁和≥60 岁）及癌细胞有无转移间的表达水平差异。对两者进行相关性分析。结果肾透明细胞癌标本OCT4 和P27kip1 蛋白阳性表达分别为16 例（66.7%）和18 例（75%）， OCT4 蛋白高、中及低表达分别为8 例（33.3%）、5 例（20.5%）及3 例（12.5%），主要在细胞质和细胞核中表达，另有少部分在胞膜中表达。P27kip1 高、中及低表达分别为3 例（12.5%）、6 例（25%）、9 例（37.5%），阳性着色在胞浆和胞膜，少部分细胞核可表达。OCT4 和P27kip1 的表达水平在年龄、性别间差异无统计学意义，在肿瘤有无转移间差异有统计学意义（P < 0.05)。OCT4 和P27kip1 在肾癌中的表达呈负相关(rs=-0.408，P < 0.05)。结论OCT4 和P27kip1 呈负相关，采用抑制OCT4 表达或P27kip1 的降解，尤其是降低胞质中P27kip1 的表达，可作为基因治疗肾癌的一个新的的作用靶点。

关键词: 基因疗法, 肾透明细胞癌, OCT4, P27kip1, 免疫组织化学

Abstract: Objective To investigate the expressions of OCT4 and P27kip1 and their correlations in renal hyaline cell carcinoma. Methods A total of 24 samples of renal hyaline cell carcinoma were detected by immunohistochemistry. The rate of cells with positive labelling of OCT4 and P27kip1 and their intra cellular distribution were observed in renal hyaline cell carcinoma. The expression levels of OCT4 and P27kip1 were compared between different gender, age (＜60y and ≥60y) and cancer cell metastasis groups. Results The rates of cells with positive OCT4 and P27kip1 expressions were 66.7% and 75% in renal hyaline cell carcinoma respectively. The ratio of cells with high, middle and low expression of OCT4 were 33.3%, 20.5% and 12.5% in renal hyaline cell carcinoma tissues. OCT4 was mainly expressed in cytoplasm and nuclei with a few was expressed in the cell membrane. The ratio of cells with high, middle and low expression of P27kip1 were 12.5%, 25% and 37.5% in renal hyaline cell carcinoma tissues respectively. The positive staining of P27kip1 was in the cytoplasm and cell membrane, and a small number of nuclei were expressed. There were no significant differences in OCT4 and P27kip1 between different age and gender groups. There were significant differences in OCT4 and P27kip1 expressions between patients with metastasis and patients without metastasis (P < 0.05). A negative correlation was found between OCT4 and P27kip1 expres⁃ sions in renal hyaline cell carcinoma (rs=-0.408, P < 0.05). Conclusion The expression of OCT4 is negatively correlated with the expression of P27kip1. Inhibiting expressions of OCT4 or P27kip1, especially knocking down P27kip1 in cytoplasm can be used as a new diagnosis and treatment of renal cancer gene therapy.

Key words: gene therapy, renal clear cell carcinoma, OCT4, P27kip1, immunohistochemistry