天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (9): 1081-1083.doi: 10.11958/20150315

• 实验研究 • 上一篇    下一篇

Rac1 和 WAVE2 蛋白在高脂饮食 C57BL/6J 幼鼠肾小球中的表达及意义

甄永煜 1， 艾浩 2， 李晓明 1,2△   

  1. 1 锦州医科大学组织胚胎学教研室（邮编 121001）；2辽宁省卵泡发育与生殖健康重点实验室
  • 收稿日期:2015-11-18 修回日期:2016-05-11 出版日期:2016-09-15 发布日期:2016-09-28
  • 通讯作者: 李晓明 E-mail:lixiaoming68327@163.com
  • 作者简介:甄永煜（1974）， 男， 硕士研究生， 主要从事肾脏发育与疾病研究
  • 基金资助:
    辽宁省高等学校优秀人才支持项目（LJQ2013092）

Expression and significance of Rac1 and WAVE2 in glomerulus of high-fat diet induced C57BL/6J model mice

ZHEN Yongyu1, AI Hao2, LI Xiaoming1,2△#br# #br#   

  1. 1 Department of Histology and Embryology, Jinzhou Medical University, Jinzhou 121001, China; 2 Liaoning Province Key Laboratory of Follicular Development and Reproductive Health
  • Received:2015-11-18 Revised:2016-05-11 Published:2016-09-15 Online:2016-09-28
  • Contact: LI Xiaoming E-mail:lixiaoming68327@163.com

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摘要： 目的 探讨 Rac1 和 WAVE2 蛋白在高脂饮食诱导的 C57BL/6J 幼鼠肾小球中的表达及意义。 方法 32 只 3 周龄雄性 C57BL/6J 幼鼠随机分为正常饮食组和高脂饮食组， 每组 16 只。 分别给予标准饲料（脂肪含量 10%）与高脂饲料（脂肪含量 60%）喂养 4 周。 HE 染色和 PAS 染色观察小鼠肾脏的病理形态学改变；应用免疫组织化学技术及蛋白免疫印迹技术检测 Rac1 和 WAVE2 蛋白的表达。 结果 与正常饮食组相比， 高脂饮食喂养的 C57BL/6J 幼鼠出现了肾小球系膜基质轻度增生以及渗出等病理改变。 同时高脂饮食组小鼠肾小球 Rac1 和 WAVE2 蛋白的表达明显增强。 结论 Rac1 和 WAVE2 蛋白可能共同参与了高脂饮食诱导的 C57BL/6J 幼鼠肾小球的损伤。

关键词: 肾小球, rac1 GTP 结合蛋白质, Wiskott-Aldrich 综合征蛋白质, 免疫组织化学, 印迹法, 蛋白质, 小鼠, 近交 C57BL, 高脂饮食, WAVE2

Abstract: Objective To explore the expression and significance of Rac1 and WAVE2 protein in glomerulus of highfat diet induced C57BL/6J model mice. Methods Thirty-two male C57BL/6J mice (3-week old) were randomly assigned into two groups(16 in each group). The control group was fed with basic diet (10% fat) for 4 weeks. The high-fat diet group was fed with high-fat diet (60% fat) for 4 weeks. The kidney morphological changes were examined by HE and PAS staining. The expressions of Rac1 and WAVE2 protein were examined by Western blot and immunohistochemistry analysis. Results HE and PAS results showed that there were glomeruli mesangial matrix hyperplasia and exudation in high- fat diet group compared with control group. The immunohistochemistry and Western blotting results showed that expressions of Rac1 and WAVE2 in glomerulus were both increased in high-fat diet group compared with those of control group. Conclusion Rac1 and WAVE2 protein may be involved in glomerular injuries induced by high-fat diet.

Key words: kidney glomerulus, rac1 GTP-binding protein, Wiskott-Aldrich syndrome protein, immunohistochemistry, blotting, Western, mice, inbred C57BL, high-fat diet, WAVE2