天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 221-225.doi: 10.11958/20150047

• 临床研究 • 上一篇    下一篇

P-JAK2/P-STAT3 与突变型 p53 蛋白在宫颈病变中的表达及临床意义

马慧 1， 尹利荣 1△， 王芳 2， 李洪林 2， 单舒 2   

  1. 天津医科大学第二医院妇科 （邮编 300211）
  • 收稿日期:2015-07-16 修回日期:2015-09-28 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: △通讯作者 E-mail:yinlirongfk@sina.com E-mail:yinlirongfk@sina.com

Expression and clinicopathological significance of P-JAK2, P-STAT3 and mutant p53 proteins in cervical lesions

MA Hui1， YIN Lirong1△， WANG Fang2， LI Honglin2， SHAN Shu2   

  1. Department of Gynecology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China
  • Received:2015-07-16 Revised:2015-09-28 Published:2016-02-15 Online:2016-02-15
  • Contact: △CorrespondingAuthor E-mail:yinlirongfk@sina.com E-mail:yinlirongfk@sina.com

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摘要： 目的 探讨宫颈病变中磷酸化 JAK 激酶 2（P-JAK2）、 磷酸化信号转导与转录因子 3(P-STAT3)及突变型 p53 蛋白的表达及其临床意义。方法 选取我科收治的宫颈鳞癌 （SCC 组） 57 例、 低级别上皮内瘤变 （LSIL 组） 36 例、高级别上皮内瘤变 （HSIL 组） 30 例和正常宫颈 （NC 组) 30 例为研究对象， 检测各组高危型人乳头瘤病毒 （HR-HPV）感染情况， 行 HE 染色、 免疫组化检测 P-JAK2、 P-STAT3 及突变型 p53 蛋白表达情况。结果 （1） SCC 组的 HRHPV 感染率、 P-JAK2 阳性表达率高于 HSIL 组、 LSIL 组及 NC 组(P＜0.05 )。（2） SCC 组的 P-STAT3、 突变型 p53 阳性表达率高于 LSIL 组、 NC 组(P＜0.05 )， 与 HSIL 组比较差异无统计学意义。（3） SCC 组的 P-JAK2 和 P-STAT3 的阳性表达率在不同的 FIGO 分期、 组织分化、 淋巴结转移、 HR-HPV 感染亚组中有明显差异（均 P＜0.05）； 突变型 p53 的阳性表达率在不同的 FIGO 分期、 HR-HPV 感染亚组中具有显著差异 （P＜0.05）。（4） SCC 组中 P-JAK2 与 P-STAT3、突变型 p53 的阳性表达率、 HR-HPV 感染率均呈正相关(P＜0.05)， P-STAT3 与突变型 p53 的表达率、 HR-HPV 感染率呈正相关(P＜0.05)； 突变型 p53 表达与 HR-HPV 感染呈正相关(P＜0.05)。结论 P-JAK2、 P-STAT3 与突变型 p53 蛋白在 SCC 组中的表达水平高于正常组及上皮内瘤变组， 三者可能与 HR-HPV 感染、 宫颈鳞癌发生、 进展有关。

关键词: 磷酸化 JAK 激酶 2, 磷酸化信号转导与转录因子 3, 突变型 p53, 宫颈鳞癌, 高危型人乳头瘤病毒, 宫颈上皮内瘤变, 免疫组织化学

Abstract: Objective To investigate the expression and prognostic significance of P-JAK2, P-STAT3 and mutant p53 in cervical lesions. Methods A total of 153 cervical biopsies of patients from Gynecology Department, The Second Hospital of Tianjin Medical University were recruited during December 2013 to June 2015. Fifty-seven cases of squamous carcinoma of cervix (SCC), 36 cases of low grade intraepithelial neoplasia (LSIL), 30 patients with high grade intraepithelial neoplasia (HSIL) and 30 cases of normal cervix (NC) were included in the study. Gene chip method was used to detect high-risk human papillpmavirus（HR-HPV） infection. Hematoxylin-eosin staining was used to make pathological diagnosis. Immunohistochemical assay was used for the detection of P-JAK2, P-STAT3 and mutant type p53 protein expression in cervical lesions. Results (1) HR-HPV infection rate and P-JAK2 expression were significantly higher in SCC group than those of HSIL group, LSIL group and NC group (P < 0.05). (2) The expression of P-STAT3 and mutant type p53 were significantly higher in SCC group than those of LSIL group and NC group (P＜0.05). However, there was no significant difference between SCC group and HSIL group. (3) The positive expressions of P-JAK2 and P-STAT3 showed significant differences in different FIGO stages, histopathological grade, lymph node metastasis and HR-HPV infection in SCC group, respectively (P < 0.05). There were significant differences in the positive expression of mutant type p53 between different FIGO stages and HR-HPV infection (P＜0.05). (4) There was positive correlation between P-JAK2, P-STAT3, positive expression of mutant type p53 and HR-HPV infection in SCC tissues (P＜0.05). There was a positive correlation between P-STAT3, p53 expression and HR-HPV infection (P < 0.05). There was a positive correlation between mutant p53 expression and HR-HPV infection (P < 0.05). Conclusion P-JAK2, P-STAT3 and mutant p53 protein expression rates are high in SCC group than those of NC and SIL groups, which may be associated with HR-HPV infection, cervical cancer occurrence and progression.

Key words: phosphorylated Janus kinase 2, phosphorylated signal transduction and transcription factor 3, mutant p53, squamous carcinoma of the cervix, high-risk human papillpmavirus, squamous intraepithelial lesion, immunohistochemistry