天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (3): 244-248.doi: 10.11958/20161361

• 实验研究 • 上一篇    下一篇

p38 MAPK 抑制剂 SB202190 对血管性痴呆大鼠海马中多种氨基酸含量的影响

王雪鹏 1， 逄锦峰 1， 陈曦 2， 何海涛 3， 庞星一 1， 赵长福 1△   

  1. 1 北华大学附属医院神经外二科 （邮编 132000）， 2 神经内二科， 3 循环内三科
  • 收稿日期:2016-11-17 修回日期:2017-01-12 出版日期:2017-03-15 发布日期:2017-03-21
  • 通讯作者: 赵长福 E-mail:xuushy@126.com
  • 基金资助:
    吉林省卫生厅课题 （20132077）； 吉林市科技局项目 （201339031）

Effects of p38 MAPK inhibitor SB202190 on contents of several kinds of amino acids in hippocampus of rats with vascular dementia

WANG Xue-peng1, PANG Jin-feng1,CHEN Xi2, HE Hai-tao3, PANG Xing-yi1, ZHAO Chang-fu1△   

  1. 1 Second Department of Neurosurgery, 2 Second Department of Neurology, 3 Third Department of Circulation,the Affiliated Hospital of Beihua University, Jilin 132000, China
  • Received:2016-11-17 Revised:2017-01-12 Published:2017-03-15 Online:2017-03-21
  • Contact: ZHAO Chang-fu E-mail:xuushy@126.com

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摘要： 摘要:目的 探讨 p38 MAPK 抑制剂 SB202190 对血管性痴呆 （VD） 大鼠海马中谷氨酸 （Glu）、 谷氨酰胺 （Gln）、 牛磺氨酸 （Tau）、 甘氨酸 （Gly） 以及 γ-氨基丁酸 （GABA） 含量的影响。方法 24 只清洁级健康雄性 3 个月龄 SD 大鼠随机分为假手术组、 VD 模型组和抑制剂组， 各 8 只。VD 模型组采用永久性结扎双侧颈总动脉法建立 VD 大鼠模型，假手术组剥离双侧颈总动脉但不结扎， 抑制剂组为大鼠建立 VD 模型后给予侧脑室注射 p38 MAPK 抑制剂SB202190。应用 Morris 水迷宫检测建模大鼠学习记忆功能， 采用微量透析法采集大鼠海马 DG 区样本并检测其多种氨基酸含量。结果 假手术组和抑制剂组一般行为学和水迷宫检测学习记忆功能明显优于 VD 模型组大鼠， 其平台潜伏期时间短于 VD 模型组， 停留平台象限时间和穿越原平台次数均显著高于 VD 模型组 （均 P＜0.05）。与假手术组相比， VD 组和抑制剂组 Glu、 Gln 及 Tau 含量明显下降， Gly 和 GABA 含量明显升高； 与 VD 模型组比较， 抑制剂组 Glu、 Gln 及 Tau 含量明显升高， Gly 和 GABA 含量明显下降 （均 P＜0.05）。结论 p38 MAPK 抑制剂对 VD 海马功能损伤具有保护作用， 可能与其能够有效调节 VD 导致的氨基酸代谢异常， 调控多种氨基酸的分泌功能有关。

关键词: 痴呆, 血管性, 海马, 氨基酸类, p38 丝裂原活化蛋白激酶类, 大鼠, Sprague-Dawley

Abstract: Abstract: Objective To investigate the effects of p38 MAPK inhibitor on glutamate (Glu), glutamine (Gln), taurine amino acid (Tau), glycine (Gly) and gamma aminobutyric acid (GABA) in hippocampus of rats with vascular dementia (VD).Methods Twenty- four healthy male SD rats were randomly divided into sham operation group, VD model group and inhibitor group. VD model was established by permanent ligation of bilateral carotid artery method, and the sham operation group stripping bilateral carotid artery but not ligation. Rats of the inhibitor group were injected p38 MAPK inhibitor SB202190 after the establishment of VD model. Morris water maze was used to evaluate the learning and memory function of rats. The samples of DG region of hippocampus were collected by microdialysis, and the contents of amino acids were detected. Results The learning and memory abilities were significantly better in sham operation group and p38 MAPK inhibitor group than those of VD model group. The latency time was significantly shorter in p38 MAPK group than that of VD model group. The time of locating the platform quadrant and the number of crossing the original platform were significantly higher in sham operation group and p38 MAPK inhibitor group than those of VD model group (P <0.05). Compared with sham group, the levels of Glu, Gln and Tau were significantly lower, and Gly and GABA were significantly higher, in VD group and p38 MAPK inhibitor group (P <0.05). Compared with VD group, Glu, Gln and Tau were significantly increased,Gly and GABA were significantly decreased in p38 MAPK inhibitor group (P <0.05). Conclusion p38 MAPK inhibitor has protective effect on VD hippocampal injury, which may be related to its ability to inhibit VD-induced abnormal secretion of amino acids and regulate the secretion of various amino acids.

Key words: dementia, vascular, hippocampus, amino acids, p38 mitogen-activated protein kinases, rats, Sprague-Dawley