天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (3): 230-234.doi: 10.11958/20181295

• 细胞与分子生物学 • 上一篇    下一篇

miR-21调控PTEN/AKT通路影响LPS诱导下足细胞中MMP-2和MMP-9表达

陈翔，黄莹，龙春艺，廖品琥   

  1. 百色市右江民族医学院附属医院呼吸科（邮编533000）
  • 收稿日期:2018-08-28 修回日期:2019-01-30 出版日期:2019-03-15 发布日期:2019-04-24
  • 通讯作者: 廖品琥 E-mail:wuhaohao234@163.com

miR-21 regulates PTEN/AKT pathway to affect the expression of MMP-2 and MMP-9 in podocytes induced by LPS

CHEN Xiang,HUANG Ying,LONG Chun-yi,LIAO Pin-hu   

  1. Department of Respiration, the Affiliated Hospital of Youjiang Medical College for Nationalities, Baise 533000, China
  • Received:2018-08-28 Revised:2019-01-30 Published:2019-03-15 Online:2019-04-24

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摘要： 目的 研究microRNA-21（miRNA-21，miR-21）在同源性磷酸酶张力蛋白（PTEN）及磷酸化蛋白激酶B（pAKT）信号通路中对脂多糖（LPS）刺激下足细胞基质金属蛋白酶（MMP）-2、MMP-9表达的影响及调控机制。方法取传3~8代足细胞，分为LPS组和对照组，LPS组给予10 mg/L LPS，对照组给予相同剂量磷酸盐缓冲液。24 h后采用实时荧光定量PCR法和ELISA法分别检测miR-21、MMP-2及MMP-9的表达，并分析miR-21和MMP-2、MMP-9的相关性。构建外源性miR-21干扰的LPS刺激下足细胞损伤模型，采用Western blot检测外源性miR-21对MMP-2、 MMP-9、PTEN和p-AKT表达的影响。结果 与对照组比较，miR-21与MMP-2、MMP-9在LPS刺激下足细胞中表达升高，两者呈正相关（P＜0.05）。外源性上调miR-21表达，MMP-2、MMP-9和p-AKT表达升高，PTEN表达降低（P＜0.05）；而外源性抑制miR-21表达，MMP-2、MMP-9和p-AKT表达降低，PTEN表达升高（P＜0.05）。结论 miR-21可通过调控PTEN/AKT通路促进LPS刺激下足细胞MMP-2和MMP-9表达。

关键词: 微 RNAs, 基质金属蛋白酶 2, 基质金属蛋白酶 9, PTEN 磷酸水解酶, 癌基因蛋白质 v-akt, 足细胞, miRNA-21

Abstract: Objective To study the effects and the regulation mechanism of microRNA-21 (miRNA-21, miR-21) on the expressions of matrix metalloproteinase 2 (MMP-2) and MMP-9 in podocytes under lipid polysaccharide (LPS) stimulation in phosphatase and tensin homology deleted on chromosome ten (PTEN) / phosphorylated protein kinase B (pAKT) signaling pathway. Methods The podocytes of 3-8 generation were divided into LPS group and control group. LPS group was given 10 mg/L LPS. The control group was given the same dose of phosphate buffer saline. After 24 h, expressions of miR-21, MMP-2 and MMP-9 were detected by real-time fluorescent quantitative PCR and ELISA, and the correlation between miR-21, MMP-2 and MMP-9 was analyzed. The model of podocyte injury interfered by exogenous miR-21 under LPS stimulation was constructed. The effects of exogenous miR-21 on expressions of MMP-2, MMP-9, PTEN and p-AKT were detected by Western blot assay. Results Compared with the control group, the expressions of miR-21, MMP-2 and MMP-9 increased in podocytes under LPS stimulation, and they were positively correlated (P＜0.05). After the expression of exogenous miR-21 was up-regulated, the expressions of MMP-2, MMP-9 and p-AKT increased but the expression of PTEN decreased (P＜0.05). While the expression of exogenous miR-21 was controlled, the expressions of MMP-2, MMP-9 and pAKT decreased but the expression of PTEN increased (P＜0.05). Conclusion The miR-21 can regulate PTEN / AKT pathway to affect the expressions of MMP-2 and MMP-9 under LPS stimulation in podocytes.

Key words: microRNAs, matrix metalloproteinase 2, matrix metalloproteinase 9, PTEN phosphohydrolase, oncogene protein v-akt, podocytes, microRNA-21