天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (5): 483-487.doi: 10.11958/20181663

• 实验研究 • 上一篇    下一篇

商陆皂苷甲对MRL/lpr小鼠iTr35细胞及其相关细胞因子表达的影响

王幸，曾慧林，张祥贵△，汤杰印   

  1. 基金项目：贵州省卫生计生委科学技术基金项目（gzwjkj2017-1-025） 作者单位：遵义医学院第五附属（珠海）医院肾内科（邮编519100） 作者简介：王幸（1992），女，硕士研究生在读，主要从事慢性肾脏病治疗研究 △通讯作者 E-mail：zxg5220@163.com
  • 收稿日期:2018-11-02 修回日期:2019-03-31 出版日期:2019-05-15 发布日期:2019-05-15
  • 通讯作者: 王幸 E-mail:609183357@qq.com
  • 基金资助:
    商陆皂苷甲对BXSB小鼠iTr35分子调控机制作用的干预研究

Effects of esculentoside A on the expression of iTr35 cells and related cytokines in MRL/lpr mice

WANG Xing, ZENG Hui-lin, ZHANG Xiang-gui△, TANG Jie-yin   

  1. Department of Nephrology, the Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai 519100, China △Corresponding Author E-mail: zxg5220@163.com
  • Received:2018-11-02 Revised:2019-03-31 Published:2019-05-15 Online:2019-05-15
  • Contact: 王幸 E-mail:609183357@qq.com

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摘要： 摘要：目的 观察商陆皂苷甲（EsA）对MRL/lpr小鼠的治疗作用和体内iTr35（CD4+Foxp3-IL-12p35+IL-27EBI3+） 细胞、白细胞介素（IL）-35及IL-17表达的影响，探讨EsA治疗狼疮性肾炎的可能机制。方法 将24只16周龄、雌性 MRL/lpr小鼠随机分为模型对照组、EsA组和EsA+IL-12p35抗体组，分别腹腔注射给药，每日1次，4周后处死小鼠， 进行尿蛋白/肌酐值、血肌酐浓度、IL-35、IL-17、iTr35细胞比例、肾脏病理的检测。结果 3组小鼠尿蛋白/肌酐值、血 肌酐浓度、IL-35、IL-17、iTr35差异有统计学意义（P＜0.05），其中模型对照组尿蛋白/肌酐值、血肌酐浓度、IL-17含量 最高，其次为EsA+IL-12p35抗体组，EsA组最低；相反，EsA组中IL-35、iTr35水平最高，其次为EsA+IL-12p35抗体 组，模型对照组最低；与模型对照组相比，EsA组和EsA+IL-12p35抗体组小鼠肾脏病理表现有一定改善。结论 狼 疮性肾炎小鼠血清中存在IL-35、IL-17的表达异常，EsA对狼疮性肾炎有效，其机制可能是通过调节IL-35、IL-17及 iTr35的表达而发挥作用的。

关键词: 商陆皂甙, 白细胞介素17, 狼疮肾炎, MRL/lpr小鼠, iTr35细胞, 白细胞介素-35

Abstract: Abstract: Objective To observe the effect of esculentoside A (EsA) on iTr35 (CD4+Foxp3-IL-12p35+IL-27EBI3+) cells and related cytokines in MRL / lpr mice, and to explore the possible mechanism of EsA in the treatment of lupus nephritis. Methods Twenty-four 16-week-old female MRL/lpr mice were randomly divided into model control group, EsA group, and EsA +IL-12p35 antibody group. The mice were intraperitoneally injected once a day. After four weeks, all mice were killed to check urine protein / creatinine value, serum creatinine concentration, IL-35, IL-17, iTr35 ratio and renal pathology. Results There were significantly differences in urine protein / creatinine value, serum creatinine concentration, IL-35, IL-17 and iTr35 ratio between the three groups (P＜0.05). The urinary protein / creatinine value, serum creatinine concentration and IL-17 levels were the highest in the model control group, followed by the EsA+IL-12p35 antibody group, and the lowest was in the EsA group. The levels of IL-35 and iTr35 were the highest in EsA group, followed by EsA + IL- 12p35 antibody group, and the lowest was in the model group. Compared with the model control group, the pathological changes of kidney were improved in EsA group and EsA + IL-12p35 antibody group. Conclusion There is abnormal expressions of IL-35 and IL-17 in lupus nephritis. EsA is effective in the treatment of lupus nephritis, and which may be through regulating the expression of IL-35, IL-17 and iTr35.

Key words: Phytolaccoside, interleukin-17, lupus nephritis, MRL/lpr mice, iTr35 cell, interleukin-35