天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (5): 488-492.doi: 10.11958/20190126

• 实验研究 • 上一篇    下一篇

机械加载对大鼠薄型子宫内膜的修复作用

田甜 1，刘大全 1，2，李心乐 1，邵珩 1，张平 1，2△   

  1. 基金项目：国家自然科学基金资助项目（81572100、81772405、81601863）；天津滨海新区卫生计生委科技资助项目（2016BWKL002、 2016BWKZ005） 作者单位：1天津医科大学基础医学院人体解剖与组织胚胎学系（邮编300070）；2北京协和医学院泰达国际心血管病医院再生医学中心 作者简介：田甜（1991），女，硕士在读，主要从事机械加载与物理康复研究 △通讯作者 E-mail：pizhang2008@163.com
  • 收稿日期:2019-01-16 修回日期:2019-03-29 出版日期:2019-05-15 发布日期:2019-05-15
  • 通讯作者: 张平 E-mail:pizhang2008@163.com
  • 基金资助:
    国家自然科学基金资助项目;国家自然科学基金资助项目;国家自然科学基金资助项目

The therapeutic effect of mechanical loading on thin endometrium in rat model

TIAN Tian1, LIU Da-quan1,2, LI Xin-le1, SHAO Heng1, ZHANG Ping1,2△   

  1. 1 Department of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; 2 Centre for Regenerative Medicine, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College △Corresponding Author E-mail: pizhang2008@163.com
  • Received:2019-01-16 Revised:2019-03-29 Published:2019-05-15 Online:2019-05-15

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摘要： 摘要：目的 探究脉冲式机械加载对薄型子宫内膜的疗效。方法 30只雌性SD大鼠随机分成假手术组（Sham 组）、薄型子宫内膜模型组（TE组）和机械加载治疗组（TE+L组），每组10只。在动情期采用95%乙醇宫腔注射法建 立薄型子宫内膜模型，Sham组宫腔注射生理盐水，TE+L组造模次日接受机械加载，加载力为5 N，频率为15 Hz，时间 为5 min/d，连续治疗15 d。HE染色观察子宫内膜组织形态变化，测量子宫内膜厚度、子宫内膜面积和腺体数目，免 疫组化染色法检测血管内皮生长因子（VEGF）和细胞增殖核抗原（Ki-67）的表达。结果 子宫组织学结果显示，TE 组子宫内膜厚度、子宫内膜面积和腺体数目较Sham组明显减少（P＜0.05），而TE+L组子宫内膜厚度、子宫内膜面积 和腺体数目较TE组明显增加（P＜0.05）。免疫组化染色结果显示，TE组子宫内膜中VEGF和Ki-67蛋白的阳性表达 率较Sham组显著降低（P＜0.05）。经过机械加载治疗后，TE+L组子宫内膜中VEGF和Ki-67蛋白的阳性表达率较TE 组显著升高（P＜0.05）。结论 机械加载能够有效修复大鼠薄型子宫内膜，该作用可能与机械加载促进子宫内膜的 细胞增殖和血管生成有关。

关键词: 子宫内膜, 血管内皮生长因子类, Ki-67抗原, 薄型子宫内膜, 机械加载, 子宫内膜修复

Abstract: Abstract: Objective To investigate the therapeutic effect of mechanical loading on thin endometrium in model rat Methods Thirty female Sprague-Dawley (SD) rats were divided randomly into sham operation group (Sham group), thi endometrium model group (TE group) and mechanical loading treatment group (TE+L group). There were ten rats in eac group. The TE model was established by injection of 95% ethanol into the uterine cavity in the estrus phase. Normal salin was injected into the uterine cavity in Sham group. Rats of TE+L group received mechanical loading on the second day afte modeling. Loading force was 5 N and the frequency was 15 Hz for 5 min/day for 15 days. HE staining was used to observe th morphological changes of endometrium. The endometrial thickness, endometrial area and gland numbers were measured. Th expressions of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (Ki-67) were evaluated b immunohistochemistry. Results Uterine histological analysis showed that endometrial thickness, endometrial area an gland numbers were significantly decreased in TE group compared with those of Sham group (P＜0.05). Compared with T group, endometrial thickness, endometrial area and gland numbers were significantly increased in TE+L group (P＜0.05 Immunohistochemistry analysis indicated that the expressions of VEGF and Ki-67 were significantly lower in th endometrium of TE group than those of Sham group P＜0.05). After mechanical loading treatment, the expressions of VEG and Ki-67 were significantly increased in the endometrium of TE+L group (P＜0.05). Conclusion Mechanical loading ca effectively repair thin endometrium in rats, which may be related to the promotion of cell proliferation and angiogenesis o endometrium.

Key words: endometrium, vascular endothelial growth factors;, Ki-67 antigen, thin endometrium, mechanical loading, endometrial repair