天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (8): 789-793.doi: 10.11958/20181947

• 细胞与分子生物学 • 上一篇    下一篇

长链非编码RNA UCA1对人下咽鳞状细胞癌FaDu 细胞增殖、侵袭、凋亡及周期的影响

张杰，唐桥斐   

  1. 1沈阳，中国人民解放军北部战区空军医院耳鼻喉科（邮编110042）；2沈阳医学院附属第二医院耳鼻喉科
  • 收稿日期:2018-12-05 修回日期:2019-05-26 出版日期:2019-08-15 发布日期:2019-08-16
  • 通讯作者: 张杰 E-mail:my000222@163.com
  • 作者简介:张杰（1980），女，博士，主治医师，主要从事耳鼻喉科研究
  • 基金资助:
    沈阳市科技局科技计划项目

Effects of long non-coding RNA UCA1 on proliferation, invasion, apoptosis and cell cycle in human hypopharyngeal squamous carcinoma cell line FaDu

ZHANG Jie，TANG Qiao-fei   

  1. 1 Department of ENT, the 463rd Hospital of the Chinese People’s Liberation Army, Shenyang 110042, China;2 Department of ENT, the Second Affiliated Hospital of Shenyang Medical College
  • Received:2018-12-05 Revised:2019-05-26 Published:2019-08-15 Online:2019-08-16

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摘要： 摘要：目的探讨长链非编码RNA尿路上皮癌抗原1（lncRNA UCA1）对人下咽鳞状细胞癌FaDu细胞增殖、侵袭、凋亡及周期的影响。方法向FaDu细胞转染UCA1-siRNA（si-UCA1组），以转染阴性干扰RNA为阴性对照组（si-NC组），以未转染组为空白对照组（Control组），通过实时荧光定量PCR确定UCA1-siRNA的干扰效果；通过CCK-8实验、Transwell侵袭实验、流式细胞术分别检测UCA1-siRNA对FaDu细胞增殖、侵袭以及细胞周期的影响；Western blot实验检测UCA1-siRNA对FaDu细胞增殖、凋亡及周期相关因子Bax、Bcl-2、cleaved-Caspase 9、cyclin D1和PCNA蛋白表达的影响。结果si-UCA1可有效下调FaDu细胞中UCA1的表达，与Control组及si-NC组相比，si-UCA1组FaDu细胞增殖及侵袭能力显著降低，G1期细胞比例显著升高，S期及G2期细胞比例显著下降，凋亡相关蛋白Bax、cleaved-Caspase 9表达水平显著升高，Bcl-2表达水平显著下调，细胞周期、增殖相关蛋白cyclin D1、PCNA表达水平显著降低（P＜0.05）。结论下调lncRNA UCA1的表达可抑制下咽鳞状细胞癌FaDu细胞增殖、侵袭能力，阻滞细胞周期，并可促进FaDu细胞凋亡。

关键词: 鼻咽肿瘤, 癌, 鳞状细胞, 细胞系, 肿瘤, RNA, 小分子干扰, 细胞增殖；细胞凋亡, 尿路上皮癌抗原1

Abstract: Abstract: Objective To investigate the effect of long non-coding RNA UCA1 on proliferation, invasion, apoptosis and cell cycle of human pharyngeal squamous cell carcinoma FaDu cells. Methods UCA1-siRNA was transfected into FaDu cells to silence UCA1, the untransfected cells were served as blank control, and transfected negative control siRNA cells were served as negative control. The interfering effect of UCA1-siRNA was determined by Real-time PCR. The effects of UCA1-siRNA on proliferation, invasion and cell cycle of FaDu cells were detected by CCK-8 assay, Transwell invasion assay and flow cytometry. Western blot assay was performed to evaluate the effect of UCA1-siRNA on the expressions of Bax, Bcl-2, cleaved-Caspase 9, cyclin D1 and PCNA proteins related to the proliferation, apoptosis and cell cycle of FaDu cells. Results Si-UCA1 effectively downregulated the expression of UCA1 in FaDu cells. After siRNA interference, the proliferation and invasion abilities of FaDu cells were significantly decreased, the proportion of cells in G1 phase was significantly increased and the proportions of cells in S phase and G2 phase were significantly decreased. The down-regulation of UCA1 by siRNA suppressed the expressions of Bcl-2, cyclin D1 and PCNA, meanwhile promoted the expressions of Bax and cleaved-Caspase 9 in FaDu cells (P＜0.05). Conclusion The downregulation of UCA1 inhibits the invasion and proliferation, blocks cell cycle, and promotes apoptosis of FaDu cells.

Key words: nasopharyngeal neoplasms, carcinoma, squamous cell, cell line, tumor, RNA, small interfering, cell proliferation, apoptosis, urothelial carcinoma associated 1