天津医药

天津医药 ›› 2021, Vol. 49 ›› Issue (3): 268-270.doi: 10.11958/20201923

• 实验研究 • 上一篇    下一篇

百里醌对辐射暴露小鼠造血系统的保护作用

刘琳琳   

  1. 天津市中西医结合医院儿内科（邮编300100）
  • 收稿日期:2020-07-07 修回日期:2020-09-14 出版日期:2021-03-15 发布日期:2021-03-15
  • 作者简介:刘琳琳（1976），女，硕士，主治医师，主要从事中西医结合治疗研究。E-mail：liulinlin0923@163.com

The protective effects of thymoquinone on the mouse hematopoietic system exposed to irradiation

LIU Lin-lin   

  1. Department of Pediatrics, Tianjin Hospital of Integrated Chinese and Western Medicine, Tianjin 300100, China
  • Received:2020-07-07 Revised:2020-09-14 Published:2021-03-15 Online:2021-03-15

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摘要： 目的　探讨百里醌对小鼠造血系统辐射损伤的影响。方法　ICR小鼠，按平均体质量一致原则分为对照组、照射组和百里醌组，每组5只。其中，对照组小鼠不接受照射，其他2组小鼠接受2.0 Gy照射。照射前1 d，对百里醌组小鼠予以灌胃百里醌（10 mg/kg）， 照射后持续3 d，其余2组小鼠灌胃生理盐水。照射7 d后处死小鼠，取外周血和单侧股骨骨髓细胞进行计数，检测骨髓细胞克隆形成能力与活性氧水平。结果　与对照组相比，照射组小鼠外周血白细胞（WBC）、红细胞（RBC）、血红蛋白（HGB）、血细胞比容（HCT）、血小板（PLT）、单侧股骨骨髓细胞数（BMMNC）、骨髓细胞集落形成数量均显著下降，骨髓细胞活性氧水平显著升高（P＜0.05），百里醌组外周血WBC、RBC、HGB、HCT和PLT计数也出现显著下降（P＜0.05）。与照射组相比，百里醌组小鼠外周血WBC、BMMNC及骨髓细胞集落形成数量显著提高（P＜0.05），骨髓细胞活性氧水平降低（P＜0.05）。结论　百里醌对造血系统辐射损伤具有保护作用，可通过抑制活性氧提高骨髓细胞增殖功能。

关键词: 全身照射, 辐射损伤, 血细胞, 活性氧, 造血系统, 骨髓细胞, 百里醌

Abstract: Objective　To observe the effects of thymoquinone on the hematopoietic radiation injury in mice. Methods　Fifteen ICR mice were randomly divided into control group, irradiated group, and thymoquinone group according to body weight. The control group received sham irradiation, and the rest received 2.0 Gy total body irradiation. Thymoquinone was intragastrically administrated with 10 mg/kg 1 d before irradiation and continued for 3 days after irradiation, the other two groups were intragastrically administered with normal saline. After 2.0 Gy irradiation for 7 day, the peripheral blood samples were collected and bone marrow nucleated cells were counted. The reactive oxygen species (ROS) and colony forming unit granulocyte- macrophage (CFU-GM) were detected. Results　Compared to the control group, white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT) and platelet (PLT), bone marrow mononuclear cells (BMMNC) and CFU-GM of mice decreased significantly in irradiated group, the ROS level increased (P＜0.05). The WBC, RBC, HGB and PLT of the peripheral blood cells significantly decreased in the thymoquinone group (P＜0.05). The WBC, BMMNC and CFU-GM were enhanced significantly in the thymoquinone group compared with those of irradiation group (P＜0.05). Conclusion　The thymoquinone shows protective effects on the radiation-induced hematopoietic damage by inhibiting ROS levels to improve the proliferation of bone marrow cells.

Key words: whole-body irradiation, radiation injuries, blood cells, reactive oxygen species, hematopoietic system, bone marrow cells, thymoquinone