蒙古黄芪皂苷对铅暴露致发育期大鼠神经炎症与肠道菌群紊乱的影响

doi:10.11958/20221821

天津医药 ›› 2023, Vol. 51 ›› Issue (9): 955-960.doi: 10.11958/20221821

蒙古黄芪皂苷对铅暴露致发育期大鼠神经炎症与肠道菌群紊乱的影响

胡明月(), 李鑫, 高磊, 关明杰^△()

内蒙古科技大学包头医学院公共卫生学院（邮编014060）

收稿日期:2022-11-07 修回日期:2023-02-14 出版日期:2023-09-15 发布日期:2023-09-13
通讯作者: △E-mail：guanmj1202@163.com
作者简介:胡明月（1996），女，硕士在读，主要从事儿童、少年卫生方面研究。E-mail：1312518494@qq.com
基金资助:
国家自然科学基金资助项目(81660546);内蒙古自然科学基金资助项目(Z19SQ2-2019MS08204)

Effects of Mongolian astragalus saponin on neuroinflammation and intestinal microflora disorder induced by lead exposure in developing rats

HU Mingyue(), LI Xin, GAO Lei, GUAN Mingjie^△()

School of Public Health, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014060, China

Received:2022-11-07 Revised:2023-02-14 Published:2023-09-15 Online:2023-09-13
Contact: △E-mail：guanmj1202@163.com

胡明月, 李鑫, 高磊, 关明杰. 蒙古黄芪皂苷对铅暴露致发育期大鼠神经炎症与肠道菌群紊乱的影响[J]. 天津医药, 2023, 51(9): 955-960.

HU Mingyue, LI Xin, GAO Lei, GUAN Mingjie. Effects of Mongolian astragalus saponin on neuroinflammation and intestinal microflora disorder induced by lead exposure in developing rats[J]. Tianjin Medical Journal, 2023, 51(9): 955-960.

摘要/Abstract

摘要：

目的探讨蒙古黄芪皂苷对铅暴露致发育期SD大鼠神经炎症及肠道菌群紊乱的干预效果。方法 40只4周龄SD大鼠随机分为对照（CG）组，铅染毒（LE）组，皂苷低、中、高剂量干预（SL、SM、SH）组，相应干预4周后，氢化物原子荧光光谱法检测大鼠血铅水平，酶联免疫吸附试验检测大鼠海马中肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-1β水平，16S rDNA高通量测序检测盲肠内容物中肠道菌群的组成。结果与CG组比较，其余各组血铅水平均升高，LE组海马中TNF-α、IL-6和IL-1β水平升高，肠道菌群α、β多样性显著改变，Shannon指数和Simpson指数降低，罗姆布茨菌属、布劳特氏菌属、毛罗菌科_UCG-001菌属相对丰度减少，而瘤胃球菌属、普雷沃菌科_UCG-001菌属相对丰度增加（P<0.05）。与LE组比较，SL、SM组血铅水平降低（P<0.05），SL组海马IL-6水平、SM组IL-1β及SM、SH组海马TNF-α、IL-6水平降低（P<0.05），SL、SM组β多样性及SM组α多样性显著改善，SM组Simpson指数升高（P<0.05），SH组、SM组瘤胃球菌属、普雷沃菌科_UCG-001菌属相对丰度减少（P<0.05），SM组罗姆布茨菌属、毛罗菌科_UCG-001菌属、布劳特氏菌属及SL组布劳特氏菌属相对丰度均增加（P<0.05）。SM组比SH组增加罗姆布茨菌丰度作用更明显（P<0.05）。结论蒙古黄芪皂苷可有效抑制铅致发育期大鼠海马中的神经炎症反应和肠道菌群的紊乱，且中剂量干预效果明显。

关键词: 铅中毒, 神经系统, 儿童, 胃肠道微生物组, 黄芪皂苷, 蒙古黄芪, 炎性因子

Abstract:

Objective To investigate the effect of Mongolian astragalus saponin on neuroinflammation and intestinal microbiota disorder caused by lead exposure in developing SD rats. Methods Forty 4-week-old Sprague Dawley rats were randomly divided into the control group (CG), the lead exposure group (LE) and the saponin intervention groups [high-dose (SH), medium-dose (SM) and low-dose (SL)]. After 4 weeks of corresponding intervention, blood lead content of rats was detected by hydride generation atomic fluorescence spectrometry. Levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) in hippocampal tissue were detected by enzyme linked immunosorbent assay. Changes of intestinal microbiota composition in cecal contents were detected by 16S rDNA sequencing. Results Compared with the CG group, blood lead levels were increased in other groups(P<0.05), levels of TNF-α, IL-6 and IL-1β in hippocampus were increased in the LE group, and the α and β diversity of intestinal flora were significantly changed in the LE group. Shannon index and Simpson index were decreased, and relative abundance of Romboutsia, Blautia and Lachnospiraceae_UCG-001 was decreased. The relative abundance of Ruminococcus and Prevotellaceae_UCG-001 was increased (P<0.05). Compared with the LE group, blood lead levels were decreased in the SL group and the SM group (P<0.05). Hippocampal IL-6 level in the SL group, the level of IL-1β in the SM group, levels of TNF-α and IL-6 in the SM group and the SH group were decreased (P<0.05). β diversity in the SL group and the SM group, and α diversity in the SM group were significantly improved, and Simpson index was increased in the SM group (P<0.05). The relative abundance of Ruminococcus and Prevotellaceae_UCG-001 decreased in the SM group and the SH group (P<0.05). The relative abundance of Romboutsia, Lachnospiraceae_UCG-001 and Blautia were significantly increased in the SM group and the SL group (P<0.05). The abundance of Romboutsia increased more significantly in the SM group than that in the SH group (P<0.05). Conclusion Mongolian astragalus saponins can effectively inhibit neuroinflammatory response and intestinal flora disorder in hippocampus of lead-induced developing rats, and the effect of medium dose intervention is obvious.

Key words: lead poisoning, nervous system, childhood, gastrointestinal microbiome, astragaloside, astragalus membranaceus bungevar.mongholicus(bunge) P.K.Hsiao, inflammatory factor

中图分类号:

R598.11

图/表 8

参考文献 23

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组别	体质量/g	血铅/（μg/L）
CG组	236.81±18.79	0.292±0.030
LE组	215.87±15.02	6.644±1.899^a
SL组	226.97±14.71	3.916±0.779^ab
SM组	237.29±12.16	3.439±1.447^ab
SH组	230.81±24.99	4.527±0.829^a
F	2.541	111.180^＊

组别	体质量/g	血铅/（μg/L）
CG组	236.81±18.79	0.292±0.030
LE组	215.87±15.02	6.644±1.899^a
SL组	226.97±14.71	3.916±0.779^ab
SM组	237.29±12.16	3.439±1.447^ab
SH组	230.81±24.99	4.527±0.829^a
F	2.541	111.180^＊

组别	TNF-α	IL-6	IL-1β
CG组	231.55±55.78	136.81±23.92	334.41±93.85
LE组	334.44±53.89^a	193.81±25.45^a	477.92±42.93^a
SL组	284.79±47.28	167.87±10.25^b	396.67±107.62
SM组	243.01±54.54^b	157.01±29.73^b	379.65±80.07^b
SH组	264.31±56.97^b	156.69±24.69^b	402.22±63.52
F	4.559^＊	6.150^＊	2.935^＊

组别	TNF-α	IL-6	IL-1β
CG组	231.55±55.78	136.81±23.92	334.41±93.85
LE组	334.44±53.89^a	193.81±25.45^a	477.92±42.93^a
SL组	284.79±47.28	167.87±10.25^b	396.67±107.62
SM组	243.01±54.54^b	157.01±29.73^b	379.65±80.07^b
SH组	264.31±56.97^b	156.69±24.69^b	402.22±63.52
F	4.559^＊	6.150^＊	2.935^＊

组别	Shannon指数	Simpson指数	chao1指数
CG组	3.78±0.74	0.79±0.08	334.39±48.60
LE组	2.93±0.62^a	0.61±0.12^a	319.18±38.18
SL组	3.12±0.53^a	0.69±0.10	356.63±66.34
SM组	3.24±0.60	0.73±0.10^b	323.82±38.67
SH组	2.98±0.37^a	0.71±0.14	325.74±31.39
F	2.788^＊	2.730^＊	0.825

蒙古黄芪皂苷对铅暴露致发育期大鼠神经炎症与肠道菌群紊乱的影响

Effects of Mongolian astragalus saponin on neuroinflammation and intestinal microflora disorder induced by lead exposure in developing rats

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参考文献 23

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组别	罗姆布茨菌属	瘤胃球菌属	毛罗菌科_UCG-001菌属	布劳特氏菌属	普雷沃菌科_UCG-001菌属
CG组	17.90（15.11，19.09）	0.21（0.15，0.25）	3.49（2.32，4.60）	0.22（0.14，0.26）	0.16（0.14，0.23）
LE组	7.71（5.67，9.14）^a	1.75（1.67，1.80）^a	0.98（0.44，1.29）^a	0.07（0.03，0.08）^a	0.82（0.75，1.01）^a
SL组	12.96（7.02，45.99）	0.42（0.26，0.56）	0.77（0.35，1.70）^a	0.20（0.12，0.27）^b	0.32（0.27，0.47）
SM组	33.92（17.06，41.87）^b	0.44（0.17，0.52）^b	2.17（1.56，2.67）^b	0.19（0.11，0.31）^b	0.23（0.10，0.39）^b
SH组	8.84（6.01，15.46）^c	0.41（0.26，0.56）^b	1.39（1.05，2.29）	0.13（0.09，0.15）	0.21（0.09，0.45）^b
H	18.434^＊	23.604^＊	22.526^＊	19.055^＊	21.980^＊

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