不同咀嚼压力对大鼠正畸移动牙压力侧牙槽骨改建的影响

doi:10.11958/20230630

天津医药 ›› 2024, Vol. 52 ›› Issue (4): 367-371.doi: 10.11958/20230630

不同咀嚼压力对大鼠正畸移动牙压力侧牙槽骨改建的影响

田静¹(), 王子龙², 肖丹娜¹^,^△(), 范向飞¹

1 天津市口腔医院正畸科（邮编300041）
2 南开大学医学院

收稿日期:2023-04-24 修回日期:2023-06-12 出版日期:2024-04-15 发布日期:2024-04-19
通讯作者: ^△E-mail：1259815407@qq.com
作者简介:田静（1987），女，主治医师，主要从事口腔正畸方面研究。E-mail：tianjing0225@163.com
基金资助:
天津市卫生健康科技项目(ZC20002)

Effects of different masticatory pressures on alveolar bone remodeling in the pressure side during orthodontic tooth movement in rats

TIAN Jing¹(), WANG Zilong², XIAO Danna¹^,^△(), FAN Xiangfei¹

1 Department of Orthodontics, Tianjin Stomatological Hospital, Tianjin 300041, China
2 School of Medicine, Nankai University

Received:2023-04-24 Revised:2023-06-12 Published:2024-04-15 Online:2024-04-19
Contact: ^△E-mail：1259815407@qq.com

田静, 王子龙, 肖丹娜, 范向飞. 不同咀嚼压力对大鼠正畸移动牙压力侧牙槽骨改建的影响[J]. 天津医药, 2024, 52(4): 367-371.

TIAN Jing, WANG Zilong, XIAO Danna, FAN Xiangfei. Effects of different masticatory pressures on alveolar bone remodeling in the pressure side during orthodontic tooth movement in rats[J]. Tianjin Medical Journal, 2024, 52(4): 367-371.

摘要/Abstract

摘要：

目的研究不同咀嚼压力对大鼠正畸移动牙压力侧牙槽骨改建的影响。方法选取8周龄雄性SD大鼠45只，按随机数字表法分为基线组5只、软食组20只和硬食组20只。基线组大鼠在实验初始处死、取材。软食组和硬食组建立上颌右侧第一磨牙近中移动模型，左侧不加力作为对照。各组喂以相应饮食，分别于加力后第3、5、7、14天各处死5只大鼠，取双侧上颌骨。Micro CT测量加力磨牙近中移动距离及压力侧牙槽骨骨体积/组织体积（BV/TV）、骨小梁分离度（Tb.Sp）和骨小梁厚度（Tb.Th）。抗酒石酸酸性磷酸酶（TRAP）染色计数破骨细胞数量；原位杂交染色观察细胞核因子-κB受体活化因子配体（RANKL）和骨保护素（OPG）mRNA表达随时间变化情况。结果第14天时软食加力组牙齿移动距离小于硬食加力组（P<0.05）。软食加力组与硬食加力组压力侧牙槽骨BV/TV、Tb.Sp、Tb.Th差异无统计学意义。细胞计数和原位杂交结果显示，在第5、7天，软食加力组的破骨细胞数量和RANKL/OPG比值均低于硬食加力组（P<0.05）。结论较小的咀嚼压力会减低大鼠正畸牙压力侧牙槽骨中的破骨活动，减小牙齿移动距离。

关键词: 咀嚼, 压力, 牙齿移动技术, 正畸学, 破骨细胞, 骨保护素, 细胞核因子-κB受体活化因子配体

Abstract:

Objective To study the effect of different masticatory pressures on alveolar bone remodeling in rats with orthodontic moving teeth. Methods Forty-five male SD rats aged 8 weeks were randomly divided into 3 groups: the baseline group (n=5), the soft diet group (n=20) and the hard diet group (n=20). Rats in the baseline group were sacrificed at the beginning of the experiment. The experimental rat models of right upper first molar movement were established in the soft diet group and the hard diet group. Left side of the maxillary was used as the control group. Each group was fed with corresponding diet. Five rats were sacrificed in each group on the day 3, day 5, day 7 and day 14 after orthodontic treatment respectively. Micro CT was used to measure the mesial movement distance of molar, bone volume/tissue volume (BV/TV), trabecular separation (Tb.Sp) and trabecular thickness (Tb.Th) of the alveolar bone on the pressure side. The number of osteoclasts in alveolar bone was counted after TRAP staining. In situ hybridization was used to detect the expression of RANKL and OPG. Results The amount of tooth movement was smaller on day 14 in the soft diet group than that of the hard diet group (P<0.05). There were no significant differences in BV/TV, Tb.SP and Tb.Th between the soft diet group and the hard diet group. TRAP staining and in situ hybridization showed that the number of osteoclasts and the RANKL/OPG ratio on day 5 and day 7 were lower in the soft diet group than those of the hard diet group (P<0.05). Conclusion The osteoclast activity in the alveolar bone on the pressure side of the orthodontic tooth is decreased and the tooth movement is inhibited under lower masticatory pressure.

Key words: mastication, pressure, tooth movement techniques, orthodontics, osteoclasts, osteoprotegerin, RANKL

中图分类号:

R783.5

图/表 8

参考文献 24

[1]	OMI M, MISHINA Y. Roles of osteoclasts in alveolar bone remodeling[J]. Genesis, 2022, 60(8/9):e23490. doi:10.1002/dvg.23490.
[2]	TSOLAKIS I A, VERIKOKOS C, PERREA D, et al. Effect of diet consistency on rat mandibular growth:a geometric morphometric and linear cephalometric study[J]. Biology(Basel), 2022, 11(6):901. doi:10.3390/biology11060901.
[3]	KARAMANI I I, TSOLAKIS I A, MAKRYGIANNAKIS M A, et al. Impact of diet consistency on the mandibular morphology:a systematic review of studies on rat models[J]. Int J Environ Res Public Health, 2022, 19(5):2706. doi:10.3390/ijerph19052706.
[4]	DENES B J, MAVROPOULOS A, BRESIN A, et al. Influence of masticatory hypofunction on the alveolar bone and the molar periodontal ligament space in the rat maxilla[J]. Eur J Oral Sci, 2013, 121(6):532-537. doi:10.1111/eos.12092.
[5]	FUJIWARA S, HORI K, SHITARA S, et al. Effect of hard gummy candy chewing on masticatory function[J]. J Oral Rehabil, 2021, 48(8):909-915. doi:10.1111/joor.13208.
[6]	TONNI I, RICCARDI G, PIANCINO M G, et al. The influence of food hardness on the physiological parameters of mastication:a systematic review[J]. Arch Oral Biol, 2020, 120:104903. doi:10.1016/j.archoralbio.2020.104903.
[7]	FUJIWARA Y, KO Y, SONODA M, et al. Effects of vitamin E and dietary conditions on the differentiation and maturation of osteoclast[J]. J Nutr Sci Vitaminol(Tokyo), 2022, 68(1):73-77. doi:10.3177/jnsv.68.73.
[8]	SATOMI K, NISHIMURA K, IGARASHI K. Semaphorin 3A protects against alveolar bone loss during orthodontic tooth movement in mice with periodontitis[J]. J Periodontal Res, 2022, 57(5):991-1002. doi:10.1111/jre.13038.
[9]	JACOX L A, TANG N, LI Y, et al. Orthodontic loading activates cell-specific autophagy in a force-dependent manner[J]. Am J Orthod Dentofacial Orthop, 2022, 161(3):423-436.e1. doi:10.1016/j.ajodo.2020.09.034.
[10]	MAVROPOULOS A, KILIARIDIS S, BRESIN A, et al. Effect of different masticatory functional and mechanical demands on the structural adaptation of the mandibular alveolar bone in young growing rats[J]. Bone, 2004, 35(1):191-197. doi:10.1016/j.bone.2004.03.020.
[11]	JANG A, WANG B, USTRIYANA P, et al. Functional adaptation of interradicular alveolar bone to reduced chewing loads on dentoalveolar joints in rats[J]. Dent Mater, 2021, 37(3):486-495. doi:10.1016/j.dental.2020.12.003.
[12]	AUNG P T, KATO C, FUJITA A, et al. Effects of low occlusal loading on the neuromuscular behavioral development of cortically-elicited jaw movements in growing rats[J]. Sci Rep, 2021, 11(1):7175. doi:10.1038/s41598-021-86581-9.
[13]	CARINA V, DELLA BELLA E, COSTA V, et al. Bone's response to mechanical loading in aging and osteoporosis:molecular mechanisms[J]. Calcif Tissue Int, 2020, 107(4):301-318. doi:10.1007/s00223-020-00724-0.
[14]	MORIISHI T, KOMORI T. Osteocytes:their lacunocanalicular structure and mechanoresponses[J]. Int J Mol Sci, 2022, 23(8):4373. doi:10.3390/ijms23084373.
[15]	GUL AMUK N, KURT G, KARSLI E, et al. Effects of mesenchymal stem cell transfer on orthodontically induced root resorption and orthodontic tooth movement during orthodontic arch expansion protocols: an experimental study in rats[J]. Eur J Orthod, 2020, 42(3):305-316. doi:10.1093/ejo/cjz035.
[16]	KIM J M, LIN C, STAVRE Z, et al. Osteoblast-osteoclast communication and bone homeostasis[J]. Cells, 2020, 9(9):2073. doi:10.3390/cells9092073.
[17]	MARCADET L, BOUREDJI Z, ARGAW A, et al. The roles of RANK/RANKL/OPG in cardiac,skeletal,and smooth muscles in health and disease[J]. Front Cell Dev Biol, 2022, 10:903657. doi:10.3389/fcell.2022.903657.
[18]	THOMPSON W R, RUBIN C T, RUBIN J. Mechanical regulation of signaling pathways in bone[J]. Gene, 2012, 503(2):179-193. doi:10.1016/j.gene.2012.04.076.
[19]	QUINN J M, WHITTY G A, BYRNE R J, et al. The generation of highly enriched osteoclast-lineage cell populations[J]. Bone, 2002, 30(1):164-170. doi:10.1016/s8756-3282(01)00654-8.
[20]	MIYAZAKI T, KURIMOTO R, CHIBA T, et al. Mkx regulates the orthodontic tooth movement via osteoclast induction[J]. J Bone Miner Metab, 2021, 39(5):780-786. doi:10.1007/s00774-021-01233-2.
[21]	ULLRICH N, SCHRöDER A, BAUER M, et al. The role of HIF-1α in nicotine-induced root and bone resorption during orthodontic tooth movement[J]. Eur J Orthod, 2021, 43(5):516-526. doi:10.1093/ejo/cjaa057.
[22]	KANZAKI H, CHIBA M, SHIMIZU Y, et al. Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis[J]. J Bone Miner Res, 2002, 17(2):210-220. doi:10.1359/jbmr.2002.17.2.210.
[23]	邹昭琪, 许彤彤, 何川, 等. 咬合力影响大鼠下颌骨微结构发育的时序观察[J]. 实用口腔医学杂志, 2019, 35(1):15-19.
	ZOU Z Q, XU T T, HE C, et al. Time series observation on microstructure development of mandibular bone under different occlusal force in rats[J]. J Pract Stomatol, 2019, 35(1):15-19. doi:10.3969/j.issn.1001-3733.2019.01.003.
[24]	WEI T, SHAN Z, WEN X, et al. Dynamic alternations of RANKL/OPG ratio expressed by cementocytes in response to orthodontic-induced external apical root resorption in a rat model[J]. Mol Med Rep, 2022, 26(1):228. doi:10.3892/mmr.2022.12744.

组别	BV/TV/%	Tb.Sp/μm	Tb.Th/μm
基线组	68.60±4.92	143.86±5.01	85.43±6.16
软食对照组	68.50±3.26	142.68±11.36	83.97±7.28
软食加力组	64.18±3.85^ab	144.34±8.01	82.71±5.94
硬食对照组	70.04±5.12	138.61±7.10	85.61±5.14
硬食加力组	63.90±5.23^ac	145.93±12.04	82.16±4.59
F	4.367^＊	0.498	0.859

组别	BV/TV/%	Tb.Sp/μm	Tb.Th/μm
基线组	68.60±4.92	143.86±5.01	85.43±6.16
软食对照组	68.50±3.26	142.68±11.36	83.97±7.28
软食加力组	64.18±3.85^ab	144.34±8.01	82.71±5.94
硬食对照组	70.04±5.12	138.61±7.10	85.61±5.14
硬食加力组	63.90±5.23^ac	145.93±12.04	82.16±4.59
F	4.367^＊	0.498	0.859

组别	第0天	第3天	第5天	第7天	第14天	F
软食对照组	0.60±0.55	1.00±1.00	1.40±0.89	1.20±0.84	0.80±0.84	0.714
软食加力组	0.60±0.55	2.00±0.71^A	3.00±0.71^ABa	5.60±0.55^ABCa	2.80±0.45^ABDa	46.389^＊＊
硬食对照组	0.60±0.55	1.00±0.71	0.80±0.45	1.20±0.84	1.00±1.00	0.481
硬食加力组	0.60±0.55	2.20±0.84^A	5.20±0.45^ABbc	7.60±0.89^ABCbc	3.40±0.89^ABCDc	65.636^＊＊
F	0.000	3.037	35.300^＊＊	83.111^＊＊	13.705^＊＊

组别	第0天	第3天	第5天	第7天	第14天	F
软食对照组	0.60±0.55	1.00±1.00	1.40±0.89	1.20±0.84	0.80±0.84	0.714
软食加力组	0.60±0.55	2.00±0.71^A	3.00±0.71^ABa	5.60±0.55^ABCa	2.80±0.45^ABDa	46.389^＊＊
硬食对照组	0.60±0.55	1.00±0.71	0.80±0.45	1.20±0.84	1.00±1.00	0.481
硬食加力组	0.60±0.55	2.20±0.84^A	5.20±0.45^ABbc	7.60±0.89^ABCbc	3.40±0.89^ABCDc	65.636^＊＊
F	0.000	3.037	35.300^＊＊	83.111^＊＊	13.705^＊＊

组别	第0天	第3天	第5天	第7天	第14天	F
软食对照组	0.011±0.001	0.011±0.002	0.011±0.001	0.012±0.004	0.012±0.003	0.069
软食加力组	0.011±0.001	0.014±0.003	0.030±0.001^ABa	0.065±0.006^ABCa	0.039±0.003^ABCDa	350.058^＊＊
硬食对照组	0.011±0.001	0.011±0.002	0.011±0.003	0.013±0.004	0.014±0.004	0.929
硬食加力组	0.011±0.001	0.014±0.003	0.040±0.005^ABbc	0.079±0.004^ABCbc	0.042±0.002^ABCDc	2 141.149^＊＊
F	0.000	1.779	187.876^＊＊	468.762^＊＊	229.560^＊＊

不同咀嚼压力对大鼠正畸移动牙压力侧牙槽骨改建的影响

Effects of different masticatory pressures on alveolar bone remodeling in the pressure side during orthodontic tooth movement in rats

引用本文

RichHTML

PDF (PC)

可视化

摘要/Abstract

使用本文

图/表 8

参考文献 24

相关文章 15

编辑推荐

Metrics

本文评价

组别	第0天	第3天	第5天	第7天	第14天	F
软食对照组	0.010±0.002	0.011±0.001	0.010±0.002	0.010±0.002	0.011±0.001	0.605
软食加力组	0.010±0.002	0.011±0.001	0.014±0.001^ABa	0.020±0.003^ABCa	0.026±0.002^ABCDa	68.662^＊＊
硬食对照组	0.010±0.002	0.011±0.001	0.010±0.001	0.011±0.001	0.011±0.001	0.580
硬食加力组	0.010±0.002	0.011±0.001	0.015±0.002^ABc	0.019±0.002^ABCb	0.027±0.002^ABCDb	106.203^＊＊
F	0.000	0.741	21.968^＊＊	47.454^＊＊	238.906^＊＊

组别	第0天	第3天	第5天	第7天	第14天	F
软食对照组	1.09±0.13	1.00±0.16	1.12±0.20	1.14±0.34	1.16±0.31	0.321
软食加力组	1.09±0.13	1.18±0.06^a	2.10±0.09^ABa	3.17±0.39^ABCa	1.53±0.09^ABCDa	113.875^＊＊
硬食对照组	1.09±0.13	1.00±0.18	1.09±0.41	1.18±0.31	1.22±0.32	0.830
硬食加力组	1.09±0.13	1.21±0.07^c	2.39±0.24^ABbc	3.97±0.36^ABCbc	1.61±0.15^ABCDc	157.670^＊＊
F	0.000	6.304^＊	43.796^＊＊	106.259^＊＊	6.685^＊

[1]	刘伟涛, 孙佳, 戚琳, 邹冰爽, 周绍楠, 周彦恒. 隐形矫治器结合种植体支抗治疗拔牙患者的垂直向变化研究[J]. 天津医药, 2022, 50(7): 726-733.
[2]	关晓航, 白玉静, 王璐, 刘晓天, 马佳君, 苍松. 青年人正常前牙牙根与牙槽骨位置的CBCT研究[J]. 天津医药, 2022, 50(4): 388-393.
[3]	易清清, 梁鹏晨, 孙苗苗, 杨荣, 梁冬雨, 沙爽, 常庆. 红景天苷抑制破骨细胞分化和极化的初步研究[J]. 天津医药, 2022, 50(3): 236-240.
[4]	雷杰, 肖遥△, 罗茂璇 . 牙合板正畸联合治疗对伴TMD的安氏Ⅱ2女性患者颞下颌关节的影响#br#[J]. 天津医药, 2021, 49(5): 520-524.
[5]	武杰, 孟昭松, 赵艳红△. 颞下颌关节紊乱病在正畸治疗中的研究进展[J]. 天津医药, 2021, 49(1): 98-102.
[6]	魏金星, 王诺鑫, 罗熠, 许艳, 肖建辉△. 植物来源天然小分子化合物防治骨质疏松症的研究进展[J]. 天津医药, 2021, 49(1): 107-112.
[7]	窦建新 , 孙丽萍 , 刘长山 , 王秀军 , 明义 , 苏亮 △. 骨保护素参与高磷诱导大鼠血管平滑肌细胞钙化调节的机制初探 #br#[J]. 天津医药, 2020, 48(5): 380-384.
[8]	陈馨, 沈小波, 彭佳美, 冯兴梅. 改良 Twin-Block矫治器对骨性安氏Ⅱ类 1分类错畸形患儿侧貌及 PAR指数的影响[J]. 天津医药, 2020, 48(3): 199-203.
[9]	刘朝晖, 马剑雄, 田爱现, 孙晓雷, 张杨, 郭悦, 马信龙. 柚皮苷对破骨细胞分化和极化影响的初步探究[J]. 天津医药, 2020, 48(12): 1141-1145.
[10]	闫常帅, 陈江琼, 王笑一, 胡芳, 张杰 . RGD靶向超声造影剂对大鼠2期压力性损伤创面的靶向性研究[J]. 天津医药, 2019, 47(9): 932-936.
[11]	关欣, 杜鑫, 万征, 杨振文, 杨孟云, 邬天凤. 肺动脉瓣收缩期跨瓣压差在评价房间隔缺损患者肺动脉收缩压中的作用[J]. 天津医药, 2018, 46(7): 715-719.
[12]	王鹏, 郭狄, 陈利华, 章礼炜, 陈海啸. 灯心草抑制RANKL诱导的破骨细胞形成[J]. 天津医药, 2018, 46(6): 624-628.
[13]	高哲 1，李心乐 1，2，李杰 1，2，张平 1，2△. Salubrinal通过调节破骨细胞发育改善骨关节炎胫骨平台不均匀沉降[J]. 天津医药, 2018, 46(4): 356-362.
[14]	林思思1,章礼炜2,孙旺1,施更生1△. 不同浓度西红花苷对RANKL诱导的小鼠破骨细胞分化的影响[J]. 天津医药, 2018, 46(2): 122-125.
[15]	信佳言 1，丛洪良 2△，赵福梅 1，宋衍秋 1. 骨保护素系统与冠状动脉粥样硬化性心脏病的研究进展[J]. 天津医药, 2018, 46(1): 104-109.