天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (7): 630-634.doi: 10.11958/20200081

• 实验研究 • 上一篇    下一篇

姜黄素类似物H8对糖尿病大鼠心脏的保护作用

仲崇琦1 ，孙文慧1 ，杨俊强1 ，王猛1 ，尚芮竹1 ，张美乐1 ，魏秀芳2 ，袁晓环3△   

  1. 1 牡丹江医学院（邮编 157011）；2 牡丹江医学院附属红旗医院；3 牡丹江医学院医药研究中心
  • 收稿日期:2020-01-08 修回日期:2020-05-10 出版日期:2020-07-15 发布日期:2020-07-16
  • 作者简介:仲崇琦（1994），女，硕士在读，主要从事内分泌系统疾病影像学及实验研究

The protective effect of curcumin analogue H8 on the heart of diabetic rats

ZHONG Chong-qi1 , SUN Wen-hui1 , YANG Jun-qiang1 , WANG Meng1 , SHANG Rui-zhu1 , ZHANG Mei-le1 , WEI Xiu-fang 2 , YUAN Xiao-huan3△   

  1. 1 Mudanjiang Medical University, Mudanjiang 157011, China; 2 Hongqi Hospital, Mudanjiang Medical University; 3 Medical Research Center, Mudanjiang Medical University
  • Received:2020-01-08 Revised:2020-05-10 Published:2020-07-15 Online:2020-07-16

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摘要： 目的 探讨姜黄素类似物H8对糖尿病大鼠心脏结构及功能的影响及机制。方法 采用随机数字表法将 24只雄性SD大鼠分为对照组、模型组和H8组，每组8只。模型组和H8组通过高脂高糖饮食诱导8周后腹腔注射链 脲佐菌素（STZ）建立2型糖尿病大鼠模型。H8组大鼠用H8（6 mg/kg）连续灌胃4周，对照组和模型组用等量羧甲基纤 维素钠连续灌胃4周。第4周末采用超声心动图评价各组大鼠心脏结构及功能变化。检测各组大鼠血液生化指标， 处死大鼠后检测心肌组织病理学改变，心肌组织中乳酸脱氢酶（LDH）、总超氧化物歧化酶（T-SOD）和丙二醛（MDA） 水平。结果 与对照组相比，模型组大鼠的左心室室壁运动减弱、心室重构明显、左室射血分数（LVEF）和短轴缩短 率（FS）降低，血糖、总胆固醇、三酰甘油水平明显升高，心肌细胞结构紊乱，心肌组织中LDH、MDA水平明显升高，T-SOD水平明显降低（P＜0.05）；与模型组相比，H8组大鼠左心室室壁运动增强、心室重构、LVEF和FS及心肌细胞排 列紊乱情况明显改善，血糖、总胆固醇、三酰甘油水平降低，心肌组织中LDH、MDA水平降低，T-SOD水平升高（P＜ 0.05）。结论 姜黄素类似物H8对糖尿病大鼠心肌损伤具有保护作用，其机制可能与抑制心室重构、改善心功能及 降低血糖、血脂及氧化应激因子水平有关。

关键词: 糖尿病, 2型；糖尿病心肌病；姜黄素；超声心动描记术；氧化性应激；姜黄素类似物

Abstract: Objective To investigate the effect and mechanism of curcumin analogue H8 on cardiac structure and function in diabetic cardiomyopathy rats. Methods Twenty-four male SD rats were randomly divided into control group, model group and H8 group with 8 rats in each group. The model group and H8 group were given high-fat and high-sugar diet for 8 weeks and then intraperitoneally injected with streptozotocin (STZ) to establish the model of type 2 diabetes mellitus. H8 group was given H8 (6 mg/kg) for 4 weeks, while the control group and model group were given the same amount of sodium carboxymethylcellulose for 4 weeks. At the end of the 4th week, echocardiography was used to evaluate the cardiac structure and function of rats in each group. Then the rats were killed. The biochemical indexes, pathological changes of myocardium, lactate dehydrogenase (LDH), total superoxide dismutase (T-SOD) and malondialdehyde (MDA) contents in myocardium were measured. Results Compared with the control group, the left ventricular wall motion was weakened, ventricular remodeling was obvious, left ventricular ejection fraction (LVEF) and fractional shortening (FS) were decreased, blood sugar, total cholesterol and triglyceride levels were significantly increased, myocardial cell structure was disordered, the contents of LDH and MDA in myocardial tissues were significantly increased, and the content of T-SOD decreased significantly in the model group (P＜0.05). Compared with the model group, the left ventricular wall motion, ventricular remodeling, LVEF, FS and cardiac arrays were significantly improved, blood sugar, total cholesterol and triglyceride levels were significantly decreased, LDH and MDA in myocardial tissues decreased, the content of T-SOD increased significantly in the rats of H8 group (P＜0.05). Conclusion Curcumin analogue H8 has a protective effect on myocardial injury in diabetic rats. Its mechanism may be related to the inhibition of ventricular remodeling, improvement of cardiac function and reduction of blood glucose, blood lipid and oxidative stress factors.

Key words: diabetes mellitus, type 2, diabetic cardiomyopathies, curcumin, echocardiography, oxidative stress; curcumin analogues