天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (9): 848-852.doi: 10.11958/20200017

• 临床研究 • 上一篇    下一篇

维生素B2在胃癌中的作用及机制研究

周俭1，唐朝亮2，胡文军1△，田添1   

  1. 1安徽省阜阳市人民医院肿瘤科（邮编236006）；2中国科技大学附属第一医院麻醉科
  • 收稿日期:2020-01-03 修回日期:2020-06-18 出版日期:2020-09-15 发布日期:2020-09-22
  • 通讯作者: 胡文军 E-mail:hwj665629@163.com
  • 基金资助:
    国家自然科学基金资助项目（81801175）

The role and mechanism of vitamin B2 in gastric cancer

ZHOU Jian1, TANG Chao-liang2, HU Wen-jun1△, TIAN Tian1   

  1. 1 Department of Oncology, Fuyang People’s Hospital, Fuyang 236006, China; 2 Department of Anesthesiology, the First Affiliated Hospital of University of Science and Technology of China
  • Received:2020-01-03 Revised:2020-06-18 Published:2020-09-15 Online:2020-09-22
  • Contact: Jian ZHOU E-mail:hwj665629@163.com

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摘要： 摘要：目的　探讨维生素B2（Vit B2）在胃癌中的表达及其生物学意义。方法　选择39例胃癌患者（胃癌组）和40例健康体检者（对照组），检测2组血清Vit B2水平，分析其与胃癌患者临床病理特征的关系。培养人胃癌MGC-803细胞，并取对数生长期细胞分别给予0、10、25、50、100 μmol/L Vit B2处理，检测细胞氧化磷酸化（葡萄糖、乳酸和琥珀酸脱氢酶）水平。实时荧光定量PCR（qPCR）检测己糖激酶Ⅱ（HKⅡ）、丙酮酸激酶M2（PKM2）和葡萄糖转运体1（GLUT1）mRNA水平。CCK-8法检测胃癌MGC-803细胞的增殖活性。流式细胞术检测胃癌细胞的凋亡情况。结果　胃癌患者血清Vit B2水平低于对照组［（207.85±39.71）μg/L vs. （246.07±45.43）μg/L］，且血清Vit B2水平与胃癌患者的TNM分期和分化程度有关；与0 μmol/L Vit B2组相比，25、50、100 μmol/L Vit B2组细胞葡萄糖消耗量和乳酸生成量均降低，琥珀酸脱氢酶含量均升高，HKⅡ、PKM2和GLUT1 mRNA水平均降低（均P＜0.05）；且50 μmol/L       Vit B2组变化最明显。对照组、Vit B2组、阿帕替尼组和联合用药组胃癌细胞增殖活性依次降低，细胞凋亡率依次升高，组间多重比较差异均有统计学意义（P＜0.05）。结论　胃癌患者血清Vit B2水平降低，Vit B2和阿帕替尼联合用药可提高其对胃癌的抗肿瘤效果。

关键词: 核黄素；胃肿瘤；癌；细胞系, 肿瘤；糖酵解；氧化磷酸化；阿帕替尼

Abstract: Abstract: Objective　To investigate the expression and biological effects of vitamin B2 in gastric cancer. Methods　Thirty-nine patients with gastric carcinoma (gastric cancer group) and 40 healthy controls (control group) were collected in this study. The serum expressions of vitamin B2 were detected in the two groups. The relationship between the level of vitamin B2 and the clinical pathological characteristics of gastric cancer patients was analyzed. Human gastric cancer MGC-803 cells were cultured and treated with 0 μmol/L, 10 μmol/L, 25 μmol/L, 50 μmol/L and 100 μmol/L vitamin B2 respectively. Glucose, lactic acid and succinate dehydrogenase levels were detected by biochemical kit. The mRNA expressions of  HKⅡ, PKM2 and GLUT1 were detected by qPCR. The cell proliferation was detected by CCK-8. Moreover, the cell apoptosis was detected by flow cytometry. Results　Compared with control group, the serum expression of vitamin B2 was significantly decreased in gastric cancer group (207.85 μg/L±39.71 μg/L vs. 246.07 μg/L±45.43 μg/L, P＜0.05). The serum expression of vitamin B2 was related with tumor TNM staging and the differentiation degree in patients with gastric cancer (P＜0.05). Compared with 0 μmol/L group, glucose consumption and lactate production were significantly decreased, while the content of succinate dehydrogenase was increased in 25 μmol/L, 50 μmol/L and 100 μmol/L groups (P＜0.05). Compared with 0 μmol/L group, HKⅡ, PKM2 and GLUT1 mRNA expressions were significantly decreased in 25 μmol/L, 50 μmol/L and 100 μmol/L groups (P＜0.05). Compared with the control group, the cell proliferation activities were decreased in turn in vitamin B2, Apatinib and combination groups (P＜0.05). Moreover, the combination could further reduce the cell proliferation activity (P＜0.05). Compared with the control group, the cell apoptosis rates were increased in turn in vitamin B2, Apatinib and combination groups (P＜0.05). Conclusion　The serum vitamin B2 levels decrease in patients with gastric cancer. The combination of vitamin B2 and apatinib could improve the anti-tumor effect on gastric cancer. 

Key words: riboflavin, stomach neoplasms, carcinoma, cell line, tumor, glycolysis, oxidative phosphorylation, apatinib

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