难治性肺炎支原体肺炎患儿外周血Galectin-3、IL-1β、IL-9表达水平及临床意义

doi:10.11958/20250076

天津医药 ›› 2025, Vol. 53 ›› Issue (4): 393-397.doi: 10.11958/20250076

难治性肺炎支原体肺炎患儿外周血Galectin-3、IL-1β、IL-9表达水平及临床意义

何倩倩(), 黄晗, 梁利红, 吴琳琳, 梁东阁()

郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院呼吸科（邮编450053）

收稿日期:2025-01-08 修回日期:2025-01-24 出版日期:2025-04-15 发布日期:2025-04-17
通讯作者: ^△ E-mail：liangdongge1989@163.com
作者简介:何倩倩（1990），女，主治医师，主要从事儿童呼吸系统感染性疾病及纤维支气管镜诊疗方面研究。E-mail：QQ9900509@163.com
基金资助:
河南省医学科技攻关计划联合共建项目(LHGJ20210683)

Expression levels and clinical significance of Galectin-3, IL-1β and IL-9 in peripheral blood of children with refractory mycoplasma pneumoniae pneumonia

HE Qianqian(), HUANG Han, LIANG Lihong, WU Linlin, LIANG Dongge()

Department of Respiratory Medicine, Children's Hospital Affiliated to Zhengzhou University/Henan Children's Hospital/Zhengzhou Children's Hospital, Zhengzhou 450053, China

Received:2025-01-08 Revised:2025-01-24 Published:2025-04-15 Online:2025-04-17
Contact: ^△ E-mail：liangdongge1989@163.com

何倩倩, 黄晗, 梁利红, 吴琳琳, 梁东阁. 难治性肺炎支原体肺炎患儿外周血Galectin-3、IL-1β、IL-9表达水平及临床意义[J]. 天津医药, 2025, 53(4): 393-397.

HE Qianqian, HUANG Han, LIANG Lihong, WU Linlin, LIANG Dongge. Expression levels and clinical significance of Galectin-3, IL-1β and IL-9 in peripheral blood of children with refractory mycoplasma pneumoniae pneumonia[J]. Tianjin Medical Journal, 2025, 53(4): 393-397.

摘要/Abstract

摘要：

目的探讨半乳糖凝集素-3（Galectin-3）、白细胞介素（IL）-1β、IL-9在难治性肺炎支原体肺炎（RMPP）患儿外周血中的表达情况及临床意义。方法选取确诊为RMPP的132例住院患儿为RMPP组，另取同期128例普通肺炎支原体肺炎（GMPP）患儿为GMPP组。收集患儿C反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α）、降钙素原（PCT）等临床资料。采用酶联免疫吸附试验（ELISA）检测入组时Galectin-3、IL-1β、IL-9表达水平；采用Pearson或Spearman法分析RMPP患儿外周血Galectin-3、IL-1β、IL-9水平与临床因素的相关性；多因素Logistic回归分析GMPP进展为RMPP的危险因素；受试者工作特征（ROC）曲线分析外周血Galectin-3、IL-1β、IL-9水平对RMPP的预测价值。结果 RMPP组热程、CRP、TNF-α、PCT、Galectin-3、IL-1β、IL-9、肺外并发症比例高于GMPP组（P<0.01）。相关性分析结果显示Galectin-3、IL-1β、IL-9水平与热程、肺外并发症、CRP、TNF-α、PCT均呈正相关（P<0.01）。Logistic回归分析结果显示，Galectin-3、IL-1β、IL-9升高是GMPP进展为RMPP的独立危险因素（P<0.05）。外周血Galectin-3、IL-1β、IL-9及三者联合预测GMPP进展为RMPP的曲线下面积（AUC）分别为0.852、0.813、0.812和0.949，联合预测效能最高（P<0.01）。结论 RMPP患儿外周血Galectin-3、IL-1β、IL-9水平均异常升高，三者联合检测可作为评估GMPP进展为RMPP的重要指标。

关键词: 肺炎, 支原体, 半乳糖凝集素3, 白细胞介素1β, 白细胞介素9

Abstract:

Objective To investigate the expression levels and clinical significance of Galectin-3, interleukin (IL)-1β and IL-9 in peripheral blood of children with refractory mycoplasma pneumoniae pneumonia (RMPP). Methods A total of 132 hospitalized children diagnosed with RMPP were selected as the RMPP group, and another 128 children with general MPP (GMPP) in the same period were selected as the GMPP group. The clinical data of C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and procalcitonin (PCT) in pediatric patients were collected. The expression levels of Galectin-3, IL-1β and IL-9 were detected by enzyme-linked immunosorbent assay (ELISA). The correlation between Galectin-3, IL-1β and IL-9 levels in peripheral blood of RMPP children and clinical factors were analyzed by Pearson or Spearman methods. Multivariate Logistic regression analysis was used to analyze risk factors of GMPP progression to RMPP. The predictive value of peripheral blood Galectin-3, IL-1β and IL-9 levels to RMPP was analyzed by receiver operating characteristic (ROC) curve. Results The heat course, CRP, TNF-α, PCT, Galectin-3, IL-1β, IL-9 and the proportion of extrapulmonary complications were higher in the RMPP group than those in the GMPP group (P<0.01). The correlation analysis results showed that Galectin-3, IL-1β and IL-9 levels were positively correlated with heat course, extrapulmonary complications, CRP, TNF-α and PCT (P<0.01). Logistic regression analysis showed that the increased levels of Galectin-3, IL-1β and IL-9 were independent risk factors for GMPP progression to RMPP (P<0.05). The area under the curve (AUC) of peripheral blood Galectin-3, IL-1β, IL-9 and their combined prediction of GMPP progression to RMPP were 0.852, 0.813, 0.812 and 0.949, respectively, and the combined prediction showed the highest efficacy (P<0.01). Conclusion The levels of Galectin-3, IL-1β and IL-9 in the peripheral blood of children with RMPP are abnormally elevated, and their combined detection can be used as an important indicator for evaluating the progression of GMPP to RMPP.

Key words: pneumonia, mycoplasma, galectin 3, interleukin-1beta, interleukin-9

中图分类号:

R725

图/表 6

参考文献 21

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组别		n		男性	年龄/岁		住院时间/d		热程/d
GMPP组		128		72（56.25）	7.25±2.30		7.50±1.85		8.05±2.15
RMPP组		132		76（57.58）	7.40±2.42		7.95±2.25		11.23±2.30
t或χ²				0.047	0.512		1.759		11.509^＊＊
组别	喘息			胸腔积液		胸膜增厚	肺外并发症		肺部湿啰音
GMPP组	21（16.41）			22（17.19）		15（11.72）	8（6.25）		23（17.97）
RMPP组	28（21.21）			25（18.94）		18（13.64）	22（16.67）		29（21.97）
χ²	0.981			0.135		0.216	6.908^＊＊		0.650
组别			CRP/（mg/L）			TNF-α/（μg/L）		PCT/（μg/L）
GMPP组			14.75±3.30			2.40±0.64		0.12±0.03
RMPP组			18.48±4.09			3.12±0.92		0.15±0.04
t			8.078^＊＊			7.305^＊＊		6.825^＊＊

组别		n		男性	年龄/岁		住院时间/d		热程/d
GMPP组		128		72（56.25）	7.25±2.30		7.50±1.85		8.05±2.15
RMPP组		132		76（57.58）	7.40±2.42		7.95±2.25		11.23±2.30
t或χ²				0.047	0.512		1.759		11.509^＊＊
组别	喘息			胸腔积液		胸膜增厚	肺外并发症		肺部湿啰音
GMPP组	21（16.41）			22（17.19）		15（11.72）	8（6.25）		23（17.97）
RMPP组	28（21.21）			25（18.94）		18（13.64）	22（16.67）		29（21.97）
χ²	0.981			0.135		0.216	6.908^＊＊		0.650
组别			CRP/（mg/L）			TNF-α/（μg/L）		PCT/（μg/L）
GMPP组			14.75±3.30			2.40±0.64		0.12±0.03
RMPP组			18.48±4.09			3.12±0.92		0.15±0.04
t			8.078^＊＊			7.305^＊＊		6.825^＊＊

组别	n	Galectin-3	IL-1β	IL-9
GMPP组	128	5.51±1.37	5.86±1.41	57.32±12.56
RMPP组	132	7.47±1.92	8.10±2.02	75.84±18.20
t		9.450^＊＊	10.339^＊＊	9.522^＊＊

组别	n	Galectin-3	IL-1β	IL-9
GMPP组	128	5.51±1.37	5.86±1.41	57.32±12.56
RMPP组	132	7.47±1.92	8.10±2.02	75.84±18.20
t		9.450^＊＊	10.339^＊＊	9.522^＊＊

变量	Galectin-3	IL-1β	IL-9
热程	0.507^＊＊	0.494^＊＊	0.497^＊＊
肺外并发症	0.512^＊＊	0.512^＊＊	0.516^＊＊
CRP	0.526^＊＊	0.548^＊＊	0.539^＊＊
TNF-α	0.538^＊＊	0.536^＊＊	0.509^＊＊
PCT	0.517^＊＊	0.527^＊＊	0.521^＊＊

难治性肺炎支原体肺炎患儿外周血Galectin-3、IL-1β、IL-9表达水平及临床意义

Expression levels and clinical significance of Galectin-3, IL-1β and IL-9 in peripheral blood of children with refractory mycoplasma pneumoniae pneumonia

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变量	β	SE	Wald χ²	P	OR	OR 95%CI
Galectin-3	1.038	0.305	11.586	0.001	2.824	1.553~5.134
IL-1β	1.069	0.401	7.109	0.008	2.913	1.327~6.393
IL-9	0.995	0.353	7.941	0.005	2.704	1.354~5.401
热程	0.076	0.215	0.125	0.724	1.079	0.708~1.644
肺外并发症	0.127	0.108	1.375	0.241	1.135	0.918~1.403
CRP	0.133	0.126	1.111	0.292	1.142	0.892~1.462
TNF-α	0.101	0.119	0.717	0.397	1.106	0.876~1.397
PCT	0.143	0.154	0.865	0.352	1.154	0.853~1.561
常数项	-30.319	7.369	9.961	0.001	0.000

变量	AUC	95%CI	截断值	敏感度/%	特异度/%
Galectin-3	0.852	0.806~0.897	6.05 ng/L	0.64	0.92
IL-1β	0.813	0.762~0.864	6.79 ng/L	0.73	0.76
IL-9	0.812	0.760~0.863	62.15 ng/L	0.80	0.70
联合	0.949	0.925~0.973		0.88	0.89

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