天津医药

• 实验研究 •    

结缔组织生长因子在单侧肾静脉结扎大鼠肾组织中的表达

宁继伟1,郑素琴2,畅继武3   

  1. 1. 大同市第三人民医院病理科
    2. 华北煤炭医学院病理教研室
    3. 天津市泌尿外科研究所
  • 收稿日期:2009-10-22 修回日期:2010-03-03 出版日期:2010-09-15 发布日期:2010-09-15
  • 通讯作者: 宁继伟

Expression of connective tissue growth factor in the renal tissue of rats with unilateral renal venous deligation.

  • Received:2009-10-22 Revised:2010-03-03 Published:2010-09-15 Online:2010-09-15

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摘要： 摘要 目的：探讨结缔组织生长因子(CTGF)在单侧肾静脉结扎大鼠肾组织中的表达及与肾小管上皮细胞表型转化的关系。方法：雄性Wistar大鼠30只，分为模型组和假手术组，模型组行左侧肾静脉结扎术，分别于术后5、10、15、20、25天处死大鼠(每组5只)。假手术组只分离不结扎。取双侧肾脏行HE、PASM和Masson染色，观察各时间点肾小管间质的改变；采用免疫组化SABC法检测角蛋白(CK)、CTGF和α-平滑肌肌动蛋白(α-SMA)在肾组织内的表达和分布；应用计算机图像分析系统对各项结果进行半定量分析，并进行t检验、单因素方差分析和相关性分析。结果：随结扎时间的延长，模型组小管间质纤维化逐渐加重，且CTGF、α-SMA的表达逐渐增加；CTGF与α-SMA的表达呈正相关；二者与胶原蛋白的表达均呈正相关。结论：在大鼠肾小管间质纤维化(Tubulointerstitial fibrosis, TIF)形成过程中，CTGF表达显著上调，并且与α-SMA、胶原蛋白的表达均呈正相关，提示CTGF可能参与了TIF及肾小管上皮细胞的表型转化。关键词 纤维化 结缔组织生长因子 α-平滑肌肌动蛋白 表型转化肾小管上皮细胞

关键词: 纤维化, 结缔组织生长因子, α-平滑肌肌动蛋白, 表型转化, 肾小管上皮细胞

Abstract: Abstract Objective: To investigate the expression of connective tissue growth factor(CTGF) in rat unilateral renal venous deligation animal model. Methods: Male Wistar rats were subjected to left renal vein ligature and sham operation, then killed at 5, 10, 15, 20 or 25 days after renal vein ligature or sham operation (n=5 at each time point). The morphological changes of tubulointerstitial fibrosis were observed by light microscopy with HE, PASM and Masson staining and the expression of CK, CTGF and α-SMA were detected in the renal tissues by immunohistochemistry. With computer aided video system, all results were run by semiquantitative analysis, t-test, mono-factor ANOVA and dependablity analysis test. Results: The extent of tubulointerstitial fibrosis became serious with the time of ligature. The expression of CTGF and α-SMA were consistent with the damage of tubulointerstitial. The positive correlation among CTGF and α- SMA or collagen protein were significant. Conclusion: These results indicated that the expression of CTGF was up-regulation obviously and had positive relationship with the expression of α-SMA and collagen protein. It was possible that CTGF participated in TIF and phenotypic transformation of tubular epithelial cells. Key words Fibrosis Connective tissue growth factor α-Smooth muscle actin Phenotype transformation Tubular epithelial cell

Key words: Fibrosis, Connective tissue growth factor, α-Smooth muscle actin, Phenotype transformation, Tubular epithelial cell