天津医药

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兔膝骨性关节炎软骨损伤及降钙素的保护作用

尤笑迎1,杨红梅2   

  1. 1. 郑州人民医院骨科
    2. 河南职工医学院
  • 收稿日期:2011-09-15 修回日期:2012-02-22 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 杨红梅

Articular Cartilage Injury in Rabbits with Knee Osteoarthritis and the Effects of Calcitonin

  • Received:2011-09-15 Revised:2012-02-22 Published:2012-07-15 Online:2012-07-15

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摘要： 目的：探讨兔膝骨性关节炎(KOA) 时的软骨损伤及降钙素(CT)的保护作用。方法:将36只健康大耳白兔随机分为空白对照组（A组）、模型组（B组）和CT治疗组（C组），B、C2组采用改良Hulth法复制膝骨性关节炎模型，A组除切开皮肤和关节腔外，不做其他处理，从术后第6周开始C组每天皮下注射CT 5 U/kg，A、B组给与等量生理盐水皮下注射，连续注射20 d后处死动物。观察关节软骨病理组织学和关节液肿瘤坏死因子（TNF）-α、一氧化氮（NO）及关节软骨Ⅱ型胶原及基质金属蛋白酶（MMP）-1的变化，以及降钙素对上述指标的影响。结果:与A组相比较，B组JP和Mankin’s评分升高，关节软骨损伤加重，关节液中TNF-α、NO水平升高（P＜0.05），关节软骨Ⅱ型胶原含量降低，MMP-1活性升高（P＜0.05）；与B组相比，C组关节液中TNF-α、NO水平降低（P＜0.05），关节软骨损伤减轻、Ⅱ型胶原含量升高，MMP-1活性降低（P＜0.05）。结论:CT可能通过抑制TNF-α、NO的表达而对KOA软骨损伤起保护作用。

关键词: 膝骨性关节炎, 肿瘤坏死因子α, 一氧化氮, Ⅱ型胶原, 基质金属蛋白酶, 降钙素

Abstract: Objective: To study the articular cartilage injury in rabbits with knee osteoarthritis and the effects of calcitonin. Methods: 36 rabbits were randomly divided into three groups: normal group (group A), model group (group B) and CT treatment group (group C).There were 12 rabbits in each group. The rabbits in group B and C were made into knee osteoarthritis by Hulth’s method. The rabbits in group A weren’t dealed by special process, except for incision of skin and articular cavity. The rabbits in group C were interfered by hypodermic injection CT everyday (5∪/kg). The other rabbits were interfered by normal saline. The pathomorphism and changes of TNF-α, NO，collagen Ⅱ and MMP-1 were observed. Results: The scores of JP and Mankin in group B were significantly higher than those in group A, articular cartilage injury was more serious, meanwhile, the content of TNF-α、NO in synovial fluid increased (P＜0.05 ), the expression of MMP-1 increased, but the expression of collagen Ⅱ decreased (P＜0.05 ). Compared with group B, the scores of JP and Mankin in group C were significantly decreased, the articular cartilage injury relieved evidently, expression of MMP-1 and the content of TNF-α, NO in synovial fluid decreased significantly (P＜ 0.05 ), but the expression of collagen Ⅱ increased significantly (P＜0.05 ). Conclusion The protection of articular cartilage injury by CT on KOA may relevant to the inhibition of TNF-α and NO.

Key words: Knee Osteoarthritis, TNF-α, NO, Collagen Ⅱ, MMP-1, Calcitonin