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参麦注射液对肠缺血再灌注肺脂质过氧化损伤的影响

王艳蕾1,崔国金2,蔡庆艳2,景友玲2,张一兵2,郑慧萍2   

  1. 1. 河北联合大学
    2. 华北煤炭医学院
  • 收稿日期:2010-03-01 修回日期:2010-06-16 出版日期:2010-11-15 发布日期:2010-11-15
  • 通讯作者: 王艳蕾

Effects of ShenMai Injection on Lipid Peroxidation in the Lung Induced by Intestinal Ischemia/Reperfusion(I/R)

  • Received:2010-03-01 Revised:2010-06-16 Published:2010-11-15 Online:2010-11-15
  • Contact: WANG Yan-Lei

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摘要： 目的：探讨参麦注射液对肠缺血/再灌注肺脂质过氧化损伤的防护作用。方法：SD大鼠24只，随机均分为对照组、模型组、治疗组。对照组只分离出肠系膜上动脉；模型组、治疗组复制大鼠肠缺血/再灌注(I/R)损伤模型；治疗组于缺血前、再灌前、再灌后30min尾静脉推注参麦注射液0.9mL/100g体质量。观察各组外周血白细胞粘附聚集（LAA）、肺组织湿/干比(W/D)、肺系数以及血浆和肺组织超氧化物歧化酶（SOD）、丙二醛（MDA）、髓过氧化物酶（MPO）的变化及参麦注射液对上述指标的影响；并进行肺组织病理学检测。结果：（1）模型组LAA、肺组织W/D、肺系数分别为（26.39±1.35）%、（3.23±0.33）、（1.065±0.163）%，均高于对照组（P <0.01），光镜下肺组织病理损害亦明显重于对照组；治疗组上述指标均明显下降，分别为（17.39±2.07））%、（2.44±0.17）、（0.751±0.064）%，与模型组比较差异显著（P <0.01）；（2）模型组血浆和肺组织SOD活性[（177.37±10.84）U/mL、（22.47±2.44）U/mgpro]低于对照组（P <0.01）；MPO活性[(0.327±0.040)U/mL、（2.961±0.314）U/g湿片]及MDA含量[(4.61±1.18) nmol/mL、（6.26±1.44）nmol/mgpro]高于对照组（P <0.01），治疗组上述各检测指标均较模型组明显改善，分别为（226.82±15.53）U/mL、（37.64±2.93）U/mgpro、(0.230±0.031)U/mL、（1.948±0.249）U/g湿片、(2.02±0.47) nmol/mL、（3.15±0.65）nmol/mgpro (P<0.01)。结论：参麦注射液对肠I/R后肺损伤具有保护作用，其机制可能与抑制中性粒细胞的聚集，对抗脂质过氧化损伤有关。

关键词: 再灌注损伤, 急性肺损伤, 脂质过氧化, 中性粒细胞, 参麦注射液

Abstract: Abstract Objective: To explore the effects of shenmai injection(SM) on lipid peroxidiation in the lung induced by intestinal ischemia/reperfusion(I/R). Methods: 24 SD rats were randomly divided into three groups:sham group,I/R group,treatment group. In sham group ,superior mesenteric artery was separated only;Models of intestinal I/R injury in I/R group and treatment group were estabished. In treatment group, SM(0.9mL/100g) was injected through tail vein before ischemia, before reperfusion and after reperfusion 30min respectively. The state of leukocyte adhesiveness/aggregation(LAA) in peripheral blood, lung wet/dry weight ratio,lung coefficient, Superoxide dismutase(SOD), malondialdehyde(MDA) , myeloperoxidase (MPO) in serum and lung tissue were measured, as well as the effects of SM were observed. The changes of lung histology were examined by light microscope. Results: (1)In I/R group ,LAA in peripheral blood, lung wet/dry weight ratio,lung coefficient were significantly increased than that in sham group(P<0.01); the damage to lung tissue was increased in severity with light microscope. The above-mentioned indexes of treatment group were obviously lower in comparison with I/R group(P<0.01).(2)The activity of SOD in serum and lung tissue were significantly lower in I/R group than that in sham group (P<0.01); The content of MDA , the activity of MPO in serum and lung tissue were significantly increased in I/R group than that in sham group (P<0.01).The above-mentioned indexes of treatment group were obviously ameliorated in comparison with I/R group(P<0.01). Conclusion: Shenmai injection can protect the lung injury induced by intestinal ischemia/reperfusion(I/R) ,which may be mediated by inhibiting lipid peroxidation and aggregation of neutrophils.

Key words: reperfusion injury, acute lung injury, lipid peroxidation, neutrophil, shenmai injection